Вопросы вирусологии. 2022; 67: 304-309
Определение оптимальной иммунизирующей дозы гетерологической вакцины вируса оспы коз (Poxviridae: Chordopoxvirinae: Capripoxvirus) против узелкового дерматита
https://doi.org/10.36233/0507-4088-116Аннотация
Введение. Узелковый дерматит, оспа овец и оспа коз являются опасными заболеваниями домашних жвачных животных, оказывающими губительное воздействие на животноводство в эндемичных регионах, нанося колоссальный экономический ущерб государству. Представители рода каприпоксвирусов антигенно схожи и могут использоваться в качестве вакцины для трёх инфекций, как в случае с представителями рода ортопоксвирусов, куда входят вирусы натуральной оспы, оспы обязан, оспы коров, составляющие единое семейство Poxviridae. Материалы и методы. В данной работе использованы вакцинный штамм G20-LKV вируса оспы коз и вирулентный штамм НИИПББ-2019/К вируса узелкового дерматита. Опыты проводили на клинически здоровом, серонегативном к поксвирусам крупном рогатом скоте (КРС) породы «Казахская белоголовая» 6–8-месячного возраста. При работе использовались вирусологические и серологические методы исследований. Результаты. Все иммунизированные животные, получившие разные дозы вакцины, проявили устойчивость к контрольному заражению вирулентным вирусом узелкового дерматита КРС без проявления каких-либо клинических признаков болезни. У животных, получивших наименьшие дозы вакцины (15 000, 30 000 и 40 000 ТЦД50), в месте введения не наблюдалось нежелательных явлений, кожной (припухлость) и температурной реакции. А у вакцинированных высокими дозами препарата (60 000 и 80 000 ТЦД50) отмечалась реакция в виде припухлостей в месте введения, которые рассасывались в течение 3–4 суток. Контрольные животные, инфицированные вирулентным вирусом, проявляли клинические признаки болезни узелкового дерматита КРС. Заключение. Вакцина, приготовленная на основе штамма G20-LKV вируса оспы коз, обладает протективными свойствами в отношении заражения КРС вирулентным вирусом узелкового дерматита. Иммунизирующая доза вакцин от 15 000 до 80 000 ТЦД50 зависит от эпизоотической ситуации узелкового дерматита в определенный момент в конкретном регионе.
Список литературы
1. Tuppurainen E.S.M., Venter E.H., Shisler J.L., Gari G., Mekonnen G.A., Juleff N., et al. Capripoxvirus diseases: current status and opportunities for control. Transbound. Emerg. Dis. 2017; 64(3): 729–45. https://doi.org/10.1111/tbed.12444.
2. Babiuk S. Vaccines against lumpy skin disease virus and vaccination strategies. In: Tuppurainen E., Babiuk S., Klement E., eds. Lumpy Skin Disease. Berlin: Springer; 2018.
3. Barnard B.J., Munz E., Dumbell K., Prozesky L. Lumpy skin disease. In: Coetzer J.A.W., Thomson G.R., Tustin R.C., eds. Infectious Diseases of Livestock. Cape Town, South Africa: Oxford University Press; 1994: 604–12.
4. Babiuk S., Bowden T.R., Boyle D.B., Wallace D.B., Kitching R.P. Capripoxviruses: An emerging worldwide threat to sheep, goats and cattle. Transbound. Emerg. Dis. 2008; 55(7): 263–72. https://doi.org/10.1111/j.1865-1682.2008.01043.x
5. Carn V.M., Kitching R.P. The clinical response of cattle experimentally infected with lumpy skin disease (Neethling) virus. Arch. Virol. 1995; 140(3): 503–13. https://doi.org/10.1007/ BF01718427
6. Abutarbush S.M., Tuppurainen E.S.M. Serological and clinical evaluation of the Yugoslavian RM65 sheep pox strain vaccine use in cattle against lumpy skin disease. Transbound. Emerg. Dis. 2018; 65(6): 1657–63. https://doi.org/10.1111/tbed.12923
7. ВОЗ. Информационный бюллетень. Оспа обезьян. Available at: https://www.who.int/ru/news-room/fact-sheets/detail/monkeypox
8. Ben-Gera J., Klement E., Khinich E., Stram Y., Shpigel N.Y. Comparison of the effcacy of Neethling lumpy skin disease virus and х10 RM65 sheep-pox live attenuated vaccines for the prevention of lumpy skin disease – the results of a randomized controlled field study. Vaccine. 2015; 33(38): 4837–42. https://doi.org/10.1016/j.vaccine.2015.07.071
9. Bhanuprakash V., Indrani B.K., Hegde R., Kumar M.M., Moorthy A.R.S. A classical live attenuated vaccine for sheep pox. Trop. Anim. Health Prod. 2004; 36(4): 307–20. https://doi.org/10.1023/B:TROP.0000026661.88631.50.
10. Tuppurainen E.S.M., Antoniou S.E., Tsiamadis E., Topkaridou M., Labus T., Debeljak Z., et al. Field observations and experiences gained from the implementation of control measures against lumpy skin disease in South-East Europe between 2015 and 2017. Prev. Vet. Med. 2020; 181: 104600. https://doi.org/10.1016/j.prevetmed.2018.12.006
11. Kitching R.P., Bhat P.P., Black D.N. The characterization of African strains of capripoxvirus. Epidemiol. Infect. 1989; 102(2): 335–43. https://doi.org/10.1017/s0950268800030016
12. Tulman E., Alfonso C., Lu Z., Zsak L., Sur J.H., Sandybaev N., et al. The genomes of sheep pox and goat pox viruses. J. Virol. 2002; 76(12): 6054–61. https://doi.org/10.1128%2FJVI.76.12.6054-6061.2002
13. Kitamura T., Kitamura Y., Tagaya I. Immunogenicity of an attenuated strain of vaccinia virus on rabbits and monkeys. Nature. 1967; 215(5106): 1187–8. https://doi.org/10.1038/2151187a0
14. Zhugunissov K., Bulatov Ye., Orynbayev M., Kutumbetov L., Abduraimov Ye., Shayakhmetov Ye., et al. Goatpox virus (G20-LKV) vaccine strain elicits a protective response in cattle against lumpy skin disease at challenge with lumpy skin disease virulent field strain in a comparative study. Vet. Microbiol. 2020; 245: 108695. https://doi.org/10.1016/j.vetmic.2020.108695.
15. Brenner J., Haimovitz M., Oron E., Stram Y., Fridgut O., Bumbarov V., et al. Lumpy skin disease (LSD) in a large dairy herd in Israel, June 2006. Isr. J. Vet. Med. 2006; 61(3/4): 73–7.
16. Coakley W., Capstick P.B. Protection of cattle against lumpy skin disease. Factors affecting small scale production of tissue culture propagated virus vaccine. Res. Vet. Sci. 1961; 2(4): 369–71. https://doi.org/10.1016/S0034-5288(18)34941-5
17. Capstick P.B. Vaccines for lumpy skin disease, sheep pox and goat pox. Dev Biol (Basel) 2003;114:161-7.
18. Capstick P.B. Veterinary Department Annual Report. Kenya; 1961: 45–47.
19. Kitching R.P., Hammond J.M., Taylor W.P. A single vaccine for the control of capripox infection in sheep and goats. Res. Vet. Sci. 1986; 42(1): 53–60.
20. Kitching R.P., Taylor W.P. Clinical and antigenic relationship between isolates of sheep and goat pox viruses. Trop. Anim. Health Prod. 1985; 17(2): 64–74. https://doi.org/10.1007/bf02360774
21. Davies F.G., Krauss H., Lund L.J., Taylor M. The laboratory diagnosis of lumpy skin disease. Res. Vet. Sci. 1971; 12(2): 123–7.
22. Gari G., Abie G., Gizaw D., Wubete A., Kidane M., Asgedom H., et al. Evaluation of the safety, immunogenicity and efficacy of three capripoxvirus vaccine strains against lumpy skin disease virus. Vaccine. 2015; 33(28): 3256–61. https://doi.org/10.1016/j.vaccine.2015.01.035
23. Aбдураимов Е.О. Разработка технологии изготовления вирусвакцины против оспы коз. Диссертация на соискания кандидата ветеринарных наук. Алматы; 2001.
Problems of Virology. 2022; 67: 304-309
Determination of the optimal immunizing dose of heterologous goat pox virus vaccine (Poxviridae: Chordopoxvirinae: Capripoxvirus) against lumpy skin disease
https://doi.org/10.36233/0507-4088-116Abstract
Introduction. Lumpy skin disease (LSD), sheep pox and goat pox are dangerous diseases of domestic ruminants. Representatives of the genus of capripoxviruses are antigenically similar and can be used as a vaccine for three infections, as in the case of representatives of the genus of orthopoxviruses, which includes viruses of smallpox, monkeypox, and cowpox, that all belong to a single family Poxviridae.
Materials and methods. In this study, the vaccine strain G20-LKV of the goat pox virus and the virulent strain RIBSP2019/K of the LSD virus were used. The experiments were carried out on clinically healthy cattle of the Kazakh White-headed breed, aged six to eight months. Virological and serological research methods were used in the work.
Results. All immunized animals that received different doses of the vaccine showed resistance to the infection challenge, without showing any clinical signs of the disease. In animals that received the lowest doses of the vaccine 15,000, 30,000 and 40,000 TCID50, no adverse events, skin and temperature reactions were observed at the injection site. Those vaccinated with high doses of the vaccine had a local reaction in the form of swelling at the site of vaccine administration. Control animals infected with a virulent virus showed clinical signs of the cattle lumpy skin disease .
Conclusion. The vaccine, prepared based on the “G20-LKV” strain of the goat virus, is protective for cattle against infection with a virulent LSD virus at immunizing doses from 15,000 to 80,000 TCID50, which are dependent on the LSD epizootic situation in particular region.
References
1. Tuppurainen E.S.M., Venter E.H., Shisler J.L., Gari G., Mekonnen G.A., Juleff N., et al. Capripoxvirus diseases: current status and opportunities for control. Transbound. Emerg. Dis. 2017; 64(3): 729–45. https://doi.org/10.1111/tbed.12444.
2. Babiuk S. Vaccines against lumpy skin disease virus and vaccination strategies. In: Tuppurainen E., Babiuk S., Klement E., eds. Lumpy Skin Disease. Berlin: Springer; 2018.
3. Barnard B.J., Munz E., Dumbell K., Prozesky L. Lumpy skin disease. In: Coetzer J.A.W., Thomson G.R., Tustin R.C., eds. Infectious Diseases of Livestock. Cape Town, South Africa: Oxford University Press; 1994: 604–12.
4. Babiuk S., Bowden T.R., Boyle D.B., Wallace D.B., Kitching R.P. Capripoxviruses: An emerging worldwide threat to sheep, goats and cattle. Transbound. Emerg. Dis. 2008; 55(7): 263–72. https://doi.org/10.1111/j.1865-1682.2008.01043.x
5. Carn V.M., Kitching R.P. The clinical response of cattle experimentally infected with lumpy skin disease (Neethling) virus. Arch. Virol. 1995; 140(3): 503–13. https://doi.org/10.1007/ BF01718427
6. Abutarbush S.M., Tuppurainen E.S.M. Serological and clinical evaluation of the Yugoslavian RM65 sheep pox strain vaccine use in cattle against lumpy skin disease. Transbound. Emerg. Dis. 2018; 65(6): 1657–63. https://doi.org/10.1111/tbed.12923
7. VOZ. Informatsionnyi byulleten'. Ospa obez'yan. Available at: https://www.who.int/ru/news-room/fact-sheets/detail/monkeypox
8. Ben-Gera J., Klement E., Khinich E., Stram Y., Shpigel N.Y. Comparison of the effcacy of Neethling lumpy skin disease virus and kh10 RM65 sheep-pox live attenuated vaccines for the prevention of lumpy skin disease – the results of a randomized controlled field study. Vaccine. 2015; 33(38): 4837–42. https://doi.org/10.1016/j.vaccine.2015.07.071
9. Bhanuprakash V., Indrani B.K., Hegde R., Kumar M.M., Moorthy A.R.S. A classical live attenuated vaccine for sheep pox. Trop. Anim. Health Prod. 2004; 36(4): 307–20. https://doi.org/10.1023/B:TROP.0000026661.88631.50.
10. Tuppurainen E.S.M., Antoniou S.E., Tsiamadis E., Topkaridou M., Labus T., Debeljak Z., et al. Field observations and experiences gained from the implementation of control measures against lumpy skin disease in South-East Europe between 2015 and 2017. Prev. Vet. Med. 2020; 181: 104600. https://doi.org/10.1016/j.prevetmed.2018.12.006
11. Kitching R.P., Bhat P.P., Black D.N. The characterization of African strains of capripoxvirus. Epidemiol. Infect. 1989; 102(2): 335–43. https://doi.org/10.1017/s0950268800030016
12. Tulman E., Alfonso C., Lu Z., Zsak L., Sur J.H., Sandybaev N., et al. The genomes of sheep pox and goat pox viruses. J. Virol. 2002; 76(12): 6054–61. https://doi.org/10.1128%2FJVI.76.12.6054-6061.2002
13. Kitamura T., Kitamura Y., Tagaya I. Immunogenicity of an attenuated strain of vaccinia virus on rabbits and monkeys. Nature. 1967; 215(5106): 1187–8. https://doi.org/10.1038/2151187a0
14. Zhugunissov K., Bulatov Ye., Orynbayev M., Kutumbetov L., Abduraimov Ye., Shayakhmetov Ye., et al. Goatpox virus (G20-LKV) vaccine strain elicits a protective response in cattle against lumpy skin disease at challenge with lumpy skin disease virulent field strain in a comparative study. Vet. Microbiol. 2020; 245: 108695. https://doi.org/10.1016/j.vetmic.2020.108695.
15. Brenner J., Haimovitz M., Oron E., Stram Y., Fridgut O., Bumbarov V., et al. Lumpy skin disease (LSD) in a large dairy herd in Israel, June 2006. Isr. J. Vet. Med. 2006; 61(3/4): 73–7.
16. Coakley W., Capstick P.B. Protection of cattle against lumpy skin disease. Factors affecting small scale production of tissue culture propagated virus vaccine. Res. Vet. Sci. 1961; 2(4): 369–71. https://doi.org/10.1016/S0034-5288(18)34941-5
17. Capstick P.B. Vaccines for lumpy skin disease, sheep pox and goat pox. Dev Biol (Basel) 2003;114:161-7.
18. Capstick P.B. Veterinary Department Annual Report. Kenya; 1961: 45–47.
19. Kitching R.P., Hammond J.M., Taylor W.P. A single vaccine for the control of capripox infection in sheep and goats. Res. Vet. Sci. 1986; 42(1): 53–60.
20. Kitching R.P., Taylor W.P. Clinical and antigenic relationship between isolates of sheep and goat pox viruses. Trop. Anim. Health Prod. 1985; 17(2): 64–74. https://doi.org/10.1007/bf02360774
21. Davies F.G., Krauss H., Lund L.J., Taylor M. The laboratory diagnosis of lumpy skin disease. Res. Vet. Sci. 1971; 12(2): 123–7.
22. Gari G., Abie G., Gizaw D., Wubete A., Kidane M., Asgedom H., et al. Evaluation of the safety, immunogenicity and efficacy of three capripoxvirus vaccine strains against lumpy skin disease virus. Vaccine. 2015; 33(28): 3256–61. https://doi.org/10.1016/j.vaccine.2015.01.035
23. Abduraimov E.O. Razrabotka tekhnologii izgotovleniya virusvaktsiny protiv ospy koz. Dissertatsiya na soiskaniya kandidata veterinarnykh nauk. Almaty; 2001.
