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Вопросы вирусологии. 2015; 60: 31-36

Исследование видовой структуры риновирусов и коронавирусов, циркулировавших в Московском регионе в период с 2007 по 2012 г.

Лободанов С. А., Киселев И. С., Аммур Ю. И., Горбаленя А. Е., Claas E. C.J., Зверев В. В., Файзулоев Е. Б.

Аннотация

Риновирусы (РВ) и коронавирусы (КВ) являются основными возбудителями острых заболеваний верхних отделов дыхательных путей. Вместе с тем они могут вызывать и более тяжелые заболевания респираторного тракта, а их виды различаться по патогенности. В данной работе изучали видопринадлежность РВ и КВ в 92 клинических образцах, положительных на эти вирусы, которые были собраны на территории Московского региона в период с 2007 по 2012 г. С помощью полимеразной цепной реакции (ПЦР) с детекцией в режиме реального времени (ПЦР-РВ) установлена циркуляция всех видов РВ (РВ-А, РВ-В и РВ-С) и КВ (КВ-NL 63, КВ-229Е, КВ-ОС43 и КВ-HKU1). Выборочное секвенирование и филогенетический анализ фрагмента 5'-нетранслируемого района (5'-НТР) 8 изолятов риновирусов выявили 4 случая РВ-С, 3 - РВ-А и 1 - РВ-В.
Список литературы

1. Turner R.B. The common cold. In: Mandell G., Bennett J., Dolin R., eds. Principles and Practice of Infectious Diseases: Volume 1. 7th ed. Philadelphia: Churchill Livingstone; 2010: 809–14.

2. Available at: http://www.niaid.nih.gov/topics/commonCold/Pages/cause.aspx.

3. García-García M.L., Calvo C., Pozo F., Villadangos P.A., PérezBreña P., Casas I. Spectrum of respiratory viruses in children with community-acquired pneumonia. Pediatr. Infect. Dis J. 2012; 31(8): 808–13.

4. Fielding B.C. Human coronavirus NL63: a clinically important virus? Future Microbiol. 2011; 6(2): 153–9.

5. Lee W.M., Lemanske R.F.Jr., Evans M.D., Vang F., Pappas T., Gangnon R. et al. Human rhinovirus species and season of infection determine illness severity. Am. J. Respir. Crit. Care Med. 2012; 186(9): 886–91.

6. Gaunt E.R., Hardie A., Claas E.C., Simmonds P., Templeton K.E. Epidemiology and clinical presentations of the four human coronaviruses 229E, HKU1, NL63 and OC43 detected over 3 years using a novel multiplex real-time PCR. J. Clin. Microbiol. 2010; 48(8): 2940–7.

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9. Eckerle I., Müller M.A., Kallies S., Gotthardt D.N., Drosten C. Invitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol. J. 2013; 10: 359.

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13. Boom R., Sol C.J., Salimans M.M., Jansen C.L., Wertheim van Dillen P.M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 1990; 28(3): 495–503.

14. Lee W.M., Kiesner C, Pappas T, Lee I, Grindle K, Jartti T. et al. A diverse group of previously unrecognized human rhinoviruses are common causes of respiratory illnesses in infants. PLoS One. 2007; 2(10): e966.

15. Kiang D., Kalra I., Yagi S., Louie J.K., Boushey H., Boothby J. et al. Assay for 5’-noncoding region analysis of all human rhinovirus prototype strains. J. Clin. Microbiol. 2008; 46(11): 3736–45.

16. Savolainen C., Blomqvist S., Mulders M.N., Hovi T. Genetic clustering of all 102 human rhinovirus prototype strains: serotype 87 is close to human enterovirus 70. J. Gen. Virol. 2002; 83(Pt 2): 333–40.

17. Linder J.E., Kraft D.C., Mohamed Y., Lu Z., Heil L., Tollefson S. et al. Human rhinovirus C: Age, season, and lower respiratory illness over the past 3 decades. J. Allergy Clin. Immunol. 2013; 131(1): 69–77.

18. Bizzintino J., Lee W.M., Laing I.A., Vang F., Pappas T., Zhang G. et al. Association between human rhinovirus C and severity of acute asthma in children. Eur. Respir. J. 2011; 37(5): 1037–42.

19. Abdul-Rasool S., Fielding B.C. Understanding human coronavirus HCoV-NL63. Open Virol. J. 2010; 4: 76–84.

20. Jevšnik M, Uršič T, Zigon N, Lusa L, Krivec U, Petrovec M. Coronavirus infections in hospitalized pediatric patients with acute respiratory tract disease. BMC Infect. Dis. 2012; 12: 365.

Problems of Virology. 2015; 60: 31-36

The prevalence of the human rhinoviruses and coronaviruses circulating in the Moscow region during 2007-2012

Lobodanov S. A., Kiselev I. S., Ammour Y. I., Gorbalenya A. E., Claas E. C. J., Zverev V. V., Faizuloev E. B.

Abstract

The rhinoviruses and coronaviruses are the most common causative agents of the acute upper respiratory tract infection in humans. They include several species that vary in the pathogenicity, some causing severe respiratory tract diseases. in this work, the species prevalence of rhinoviruses and coronaviruses was studied in 92 virus-positive clinical patients that were collected at the area of the Moscow region during the period from 2007 to 2012. Using the real-time PCR the virus circulation has been established for all species common in humans, including three rhinoviruses, HRV A, HRV B, and HRV C, and four coronaviruses, HCoV-NL63, HCoV-229E, HCoV- OC43, and HCoV-HKU1. For eight patients, the identity of the rhinoviruses, including 4 cases of HRV-C, 3 cases of HRV-A, and a single case of hRv-B, was corroborated using partial sequencing of the 5 non-coding regions and phylogenetic analysis. The viruses of HRV-C, HCoV-NL63, and HCoV-OC43 were prevalent in children with severe respiratory diseases.
References

1. Turner R.B. The common cold. In: Mandell G., Bennett J., Dolin R., eds. Principles and Practice of Infectious Diseases: Volume 1. 7th ed. Philadelphia: Churchill Livingstone; 2010: 809–14.

2. Available at: http://www.niaid.nih.gov/topics/commonCold/Pages/cause.aspx.

3. García-García M.L., Calvo C., Pozo F., Villadangos P.A., PérezBreña P., Casas I. Spectrum of respiratory viruses in children with community-acquired pneumonia. Pediatr. Infect. Dis J. 2012; 31(8): 808–13.

4. Fielding B.C. Human coronavirus NL63: a clinically important virus? Future Microbiol. 2011; 6(2): 153–9.

5. Lee W.M., Lemanske R.F.Jr., Evans M.D., Vang F., Pappas T., Gangnon R. et al. Human rhinovirus species and season of infection determine illness severity. Am. J. Respir. Crit. Care Med. 2012; 186(9): 886–91.

6. Gaunt E.R., Hardie A., Claas E.C., Simmonds P., Templeton K.E. Epidemiology and clinical presentations of the four human coronaviruses 229E, HKU1, NL63 and OC43 detected over 3 years using a novel multiplex real-time PCR. J. Clin. Microbiol. 2010; 48(8): 2940–7.

7. Available at: http://www.ictvonline.org/virusTaxonomy.asp.

8. de Groot R.J., Baker S.C., Baric R.S., Brown C.S., Drosten C., Enjuanes L. et al. Middle East respiratory syndrome coronavirus (MERS-CoV): announcement of the Coronavirus Study Group. J. Virol. 2013; 87(14): 7790–2.

9. Eckerle I., Müller M.A., Kallies S., Gotthardt D.N., Drosten C. Invitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol. J. 2013; 10: 359.

10. World Health Organization. Global alert and response. Middle East respiratory syndrome coronavirus (MERS-CoV) – update. 27 December 2013. Available at: www.who.int/csr/don/2013_12_27/en/index.html (accessed 9 January 2014).

11. Lobodanov S.A. Effektivnost' etiologicheskoi diagnostiki ORVI metodom PTsR s detektsiei v rezhime real'nogo vremeni: Diss. ... kand. biol. nauk. M.; 2013.

12. Faizuloev E.B., Lobodanov S.A., Nikonova A.A., Kaira A.N., Polukhina G.M., Trushakova S.V. i dr. Differentsial'naya diagnostika ORVI metodom mul'tipleksnoi PTsR s detektsiei v rezhime real'nogo vremeni. Epidemiologiya i vaktsinoprofilaktika. 2012; 1: 12–8.

13. Boom R., Sol C.J., Salimans M.M., Jansen C.L., Wertheim van Dillen P.M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 1990; 28(3): 495–503.

14. Lee W.M., Kiesner C, Pappas T, Lee I, Grindle K, Jartti T. et al. A diverse group of previously unrecognized human rhinoviruses are common causes of respiratory illnesses in infants. PLoS One. 2007; 2(10): e966.

15. Kiang D., Kalra I., Yagi S., Louie J.K., Boushey H., Boothby J. et al. Assay for 5’-noncoding region analysis of all human rhinovirus prototype strains. J. Clin. Microbiol. 2008; 46(11): 3736–45.

16. Savolainen C., Blomqvist S., Mulders M.N., Hovi T. Genetic clustering of all 102 human rhinovirus prototype strains: serotype 87 is close to human enterovirus 70. J. Gen. Virol. 2002; 83(Pt 2): 333–40.

17. Linder J.E., Kraft D.C., Mohamed Y., Lu Z., Heil L., Tollefson S. et al. Human rhinovirus C: Age, season, and lower respiratory illness over the past 3 decades. J. Allergy Clin. Immunol. 2013; 131(1): 69–77.

18. Bizzintino J., Lee W.M., Laing I.A., Vang F., Pappas T., Zhang G. et al. Association between human rhinovirus C and severity of acute asthma in children. Eur. Respir. J. 2011; 37(5): 1037–42.

19. Abdul-Rasool S., Fielding B.C. Understanding human coronavirus HCoV-NL63. Open Virol. J. 2010; 4: 76–84.

20. Jevšnik M, Uršič T, Zigon N, Lusa L, Krivec U, Petrovec M. Coronavirus infections in hospitalized pediatric patients with acute respiratory tract disease. BMC Infect. Dis. 2012; 12: 365.