ВЫДЕЛЕНИЕ И ФИЛОГЕНЕТИЧЕСКИЙ АНАЛИЗ КАЛИЦИВИРУСА КОШЕК В СИБИРИ

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Вопросы вирусологии. 2018; 63: 268-274

ВЫДЕЛЕНИЕ И ФИЛОГЕНЕТИЧЕСКИЙ АНАЛИЗ КАЛИЦИВИРУСА КОШЕК В СИБИРИ

Глотова Т. И., Семёнова О. В., Никонова А. А., Глотов А. Г., Вяткин Ю. В., Бондарь А. А.

https://doi.org/10.18821/0507-4088-2018-63-6-268-274

Аннотация

Представлены результаты изучения распространения калицивирусной инфекции в популяции кошек разных пород, содержащихся индивидуально или группами, выделения изолятов вируса в культуре клеток и филогенетического анализа их нуклеотидных последовательностей в сравнении с опубликованными последовательностями референсных эпизоотических и вакцинных штаммов Feline calicivirus. Клинические признаки инфекции установлены у 14,3% обследованных животных. В первично-трипсинизированной культуре клеток почки эмбриона котёнка выделили 7 цитопатогенных изолятов вируса: 1 от кошки с острой формой инфекции, 5 от животных с субклинической формой и 1 от кошки с системной формой болезни. Они адаптированы к перевиваемой культуре клеток почки котёнка FK-81, в которой накапливались в титрах 10,0 ± 1,15 lg ТЦД50/см3. На основе анализа последовательностей региона второй открытой рамки считывания вирусного генома установлена высокая степень идентичности сибирского штамма Eshli и китайского штамма КМ016908, равная 81,0%. Изоляты, выделенные от животных в Сибири, генетически отличаются от штаммов, входящих в состав импортных вакцин, применяемых для профилактики болезни в нашей стране, а также друг от друга. В питомниках, не имеющих контактов и связей друг с другом, но находящихся в одном географическом регионе, популяции вируса калицивироза кошек (FCV) могут иметь некоторые генетические отличия. Выявлено близкое родство некоторых полевых изолятов со штаммами из других стран, территориально удалённых от региона Сибири. Исследования по молекулярной эпизоотологии калицивирусов важны при разработке тест-систем и мониторинге распространения штаммов на территории РФ.

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51. Wardley R.C. Feline calicivirus carrier state: a study of the host/virus Relationship. Arch. Virol. 1976; 52(3): 243-9.

52. Edgar R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic. Acids Res. 2004; 32(5): 1792-7.

53. Guindon S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W., Gascuel O. New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Syst. Biol. 2010; 59(3): 307-11.

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55. Wensman J.J., Samman A., Lindhe A., Thibault J.C., Berndtsson L.T., Hosie M.J. Ability of vaccine strain induced antibodies to neutralize field isolates of caliciviruses from Swedish cats. Acta Vet. Scand. 2015; 57: 86.

56. Bittle J.L., York C.J., Newberne J.W., Martin M. Serologic relationship of new feline cytopathogenic viruses. Am. J. Vet. Res. 1960; 21: 547-50.

57. Kahn D.E., Gillespie J.H. Feline Viruses. X. Characterization of a newly-isolated picornavirus causing interstitial pneumonia and ulcerative stomatitis in the domestic cat. Cornell Vet. 1970; 60(4): 669-3.

58. Willi B., Spiri A.M., Meli M.L., Samman A., Hoffmann K., Sydler T., et al. Molecular characterization and virus neutralization patterns of severe, non-epizootic forms of feline calicivirus infections resembling virulent systemic disease in cats in Switzerland and in Liechtenstein. Vet. Microbiol. 2016; 182: 202-12.

Problems of Virology. 2018; 63: 268-274

ISOLATION AND PHYLOGENETIC ANALYSIS OF FELINE CALICIVIRUS IN SIBERIA

Glotova T. I., Semenova O. V., Nikonova A. A., Glotov A. G., Vyatkin Y. V., Bondar A. A.

https://doi.org/10.18821/0507-4088-2018-63-6-268-274

Abstract

The results of the study of the distribution of calicivirus infection in a population of domestic cats of different breeds, contained individually or the group method, the virus isolation in the cell culture and a comparative phylogenetic analysis of their nucleotide sequences with published sequences of reference field and vaccine strains of Feline calicivirus (FCV) from other countries: USA, Germany, Japan, China and Korea are presented. Clinical signs of infection were found in 14.3% of the animals examined. After several passages in the primary kidney cells of the kitten embryo, seven cytopathogenic isolates FCV were isolated: 1 - from a cat with an acute infection, 5 - subclinical infection, 1 - systemic infection. They were adapted to continuous FK-81 cells in which they reached a maximum infectious activity of 10.0 ± 1.15 lg TCD 50 / cm3. Based on the sequence analysis of the open reading frame 2 region of the viral genome Eshli strain showed a close relationship with strain KM016908 from China with the identity of the nucleotide sequences between them of 81.0%. The results of the investigations showed that FCV isolates obtained from animals on the territory of Siberia are genetically different from strains included to imported vaccines used to prevent disease in Russian Federation and also among themselves. This causes a decrease in the effectiveness of preventive measures. In nurseries that do not have contacts and connections between themselves but located in the same geographic region FCV populations may have some genetic differences. A close relationship of some field isolates with strains from other countries geographically located so far from the Siberian region has been revealed. Studies on the molecular epizootology of caliciviruses are important in the development of test systems and the monitoring of the spread of strains in Russia.

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