Вестник Томского государственного университета. Биология. 2019; : 128-141
Effect of bacterization with Aeromonas media GS4 and Pseudomonas extremorientalis PhS1 on wheat seedlings under different abiotic conditions
https://doi.org/10.17223/19988591/45/7Аннотация
We studied the effect of soft wheat seed treatment (Triticum aestivum L.) with two bacterial strains (Aeromonas media GS4 and Pseudomonas extremorientalis PhS1) isolated from earthworm coprolites on the growth and development of wheat seedlings in a 12-day laboratory experiment, as well as on root rot disease and the activity of guaiacoldependant peroxidase under optimal conditions and abiotic stress (elevated and low temperatures and moisture content). We established that growing nonbacterized wheat plants under stress abiotic conditions reduced the height of plants compared to growing under optimal abiotic conditions, and seed bacterization with P. extremorientalis PhS1 strain increased wheat plant height (by 9-15%) under stress abiotic conditions compared to the nonbacterized plants. Bacterization with both strains decreased infestation of wheat seedlings (2.5-4 times) by root rots under unfavorable abiotic conditions compared to nonbacterized plants. In addition, under optimal and arid conditions, bacterization with P. extremorientalis PhS1 strain was the most effective, and under humid conditions it was bacterization with A. media GS4 strain. We showed that the activity of guaiacoldependant peroxidase correlates with the development of plant resistance to abiotic stress. In our experiments, plant bacterization resulted in a 2-fold increase in peroxidase activity both in leaves and roots of wheat plants compared to the nonbacterized plants. As the result, the ability of bacteria to activate peroxidase can serve as an information indicator of strengthening protective mechanisms of plants during bacterization. The paper contains 4 Figures and 34 References.
Список литературы
1. Gontia-Mishra I, Sapre S, Sharma A, Tiwari S. Amelioration of drought tolerance in wheat by the interaction of plant growth-promoting rhizobacteria. Plant Biology. 2016;18(6):992- 1000. doi: 10.1111/plb.12505
2. Choudhary DK, Kasotia A, Jain S, Vaishnav A, Kumari S, Sharma KP, Varma A. Bacterialmediated tolerance and resistance to plants under abiotic and biotic stresses. J Plant Growth Regul. 2016;35:276-300. doi 10.1007/s00344-015-9521-x
3. Kumar A, Verma JP. Does plant-microbe interaction confer stress tolerance in plants: A review? Microbiological Research. 2018;207:41-52. doi: 10.1016/j.micres.2017.11.004
4. Javadian N, Karimzadeh G, Mahfoozi S, Ghanati F. Cold induced changes of enzymes, proline, carbohydrates, and chlorophyll in wheat. Russ J Plant Physiol. 2010;57(4):540-547.
5. Kasim WA, Osman ME, Omar MN, Abd El-Daim IA, Bejai S, Meijer J. Control of drought stress in wheat using plant-growth-promoting bacteria. J Plant Growth Regul. 2013;32:122- 130. doi: 10.1007/s00344-012-9283-7
6. Khalilzadeh R, Sharif RS, Jalilian J. Antioxidant status and physiological responses of wheat (Triticum aestivum L.) to cycocel application and bio fertilizers under water limitation condition. J Plant Interactions. 2016;11(1):130-137. doi: 10.1080/17429145.2016.1221150
7. Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. 2010;48(12):909-930. doi: 10.1016/j.plaphy.2010.08.016
8. Kreslavski VD, Los DA, Allakhverdiev SI, Kuznetsov VlV. Signaling role of reactive oxygen species in plants under stress. Russ J Plant Physiol. 2012;59(2):141-154. doi: 10.1134/S1021443712020057
9. Perez IB, Brown PJ. The role of ROS signaling in cross-tolerance: From model to crop. Front Plant Sci. 2014;5:754-. doi: 10.3389/fpls.2014.00754
10. Baxter A, Mittler R, Suzuki N. ROS as key players in plant stress signalling. J Experimental Botany. 2013;65(5):1229-40. doi: 10.1093/jxb/ert375
11. Shalygo NV, Domanskaya IN, Radyuk MS, Shcherbakov RA, Dremuk IA. Accumulation of hydrogen peroxide and functioning of defense system in overwatered barley seedlings. Russ J Plant Physiol. 2012;59(6);748-756. doi: 10.1134/S1021443712050147
12. Maksimov IV, Cherepanova EA, Burkhanova GF, Sorokan AV, Kuz’mina OI. Structural and functional features of plant isoperoxidases. Biochemistry (Moscow). 2011;76(6):609- 621. doi: 10.1134/S0006297911060010
13. Dimkpa C, Weinand T, Asch F. Plant-rhizobacteria interactions alleviate abiotic stress conditions. Plant, Cell and Environment. 2009;32:1682-1694. doi: 10.1111/j.1365- 3040.2009.02028.x
14. de Souza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology. 2015;38(4):401-419. doi: 10.1590/S1415-475738420150053
15. Maksimov IV, Veselova SV, Nuzhnaya TV, Sarvarova ER, Khairullin RM. Plant growth promoting bacteria in regulation of plant resistance to stress factors. Russ J Plant Physiol. 2015;62:715-726.
16. Minaeva OM, Akimova EE, Tereshchenko NN, Zyubanova TI, Apenysheva MV, Kravets AV. Effect ofPseudomonasbacteria on peroxidase activity in wheat plants when infected withBipolaris sorokiniana. Russ J Plant Physiol. 2018;65(5):366-375. doi: 10.1134/S1021443718040052
17. Sukkasem P, Kurniawan A, Kao T-C, Chuang H-W. A multifaceted rhizobacterium Bacillus licheniformis functions as a fungal antagonist and a promoter of plant growth and abiotic stress tolerance. Environmental and Experimental Botany. 2018;155:541-551. doi: 10.1016/j.envexpbot.2018.08.005
18. Abd El-Daim IA, Bejai S, Fridborg I, Meijer J. Identifying potential molecular factors involved in Bacillus amyloliquefaciens 5113 mediated abiotic stress tolerance in wheat. Plant Biology. 2018;20(2):271-279. doi: 10.1111/plb.12680
19. Tereshchenko N, Akimova E, Minaeva O, Kravets A, Zyubanova T. Presowing with bacteria improved the productivity and resistance to fungal root pathogen in wheat and barley. In: Grasses as Food and Feed. Tadele Z, editor. London, United Kingdom: IntechOpen Publ.; 2018. pp. 153-167. doi: 10.5772/intechopen.80084
20. Minaeva OM, Akimova EE, Tereshchenko NN, Kravets AV, Zyubanova TI, Apenysheva MV. Pseudomonads associated with soil lumbricides as promising agents in root rod biocontrol for spring grain crops. Sel’skokhozyaistvennaya biologiya = Agricultural Biology. 2019;54(1):91-100. doi: 10.15389/agrobiology.2019.1.91rus In Russian, English Summary
21. Minaeva OM, Akimova EE. Effectiveness of applying bacteria Pseudomonas sp., strain B-6798, for anti-phytopathogenic protection of crops in Western Siberia. Vestnik Tomskogo Gosudarstvennogo Universiteta. Biologiya = Tomsk State University Journal of Biology. 2013;3(23):19-37.
22. Baslavskaya SS, Trubetskova OM. Praktikum po fziologii rasteniy [Practical Works on Plant Physiology]. Moscow: Moscow State Univ. Publ.; 1964. 326 р. In Russian
23. Cooke BM. Disease assessment and yield loss. In: The Epidemiology of Plant Diseases. Cooke BM, Jone DG, Kaye B, editors. 2nd ed. Dorchert: Springer Publ.; 2006. pp. 43- 80.
24. Chupakhina GN. Fiziologicheskie i biokhimicheskie metody analiza rasteniy: Praktikum [Physiological and biochemical methods of plant analysis: Workshop]. Kaliningrad: Kaliningrad Univ. Publ.; 2000. 59 p. In Russian
25. Chance B, Maehly AC. Assays of catalases and peroxidases. In: Methods in Enzymology. Colowick SP and Kaplan NO, editors. New York: Academic Press; 1955. Vol II. pp. 764- 775.
26. Kovalev VM. Teoriya urozhaya [Harvesting theory]. 3rd ed. Мoscow: Moscow Agricultural Academy Publ.; 2003. 332 p. In Russian
27. Acevedo E, Silva P, Silva H. Wheat growth and physiology. In: Bread wheat Improvement and production. Curtis BC, Rajaram S and Gomez Macpherson H, editors. Rome: FAO; 2002. pp. 39-70. Available at: http://www.fao.org/3/Y4011E/y4011e00.htm#Contents (access 15.01.2019).
28. Arraktham S, Tancho A, Niamsup P, Rattanawaree P. The potential of bacteria isolated from earthworm intestines, vermicompost and liquid vermicompost to produce indole-3-acetic acid (IAA). J Agricultural Technology. 2016;12(2):229-239.
29. Aarab S, Ollero FJ, Megías M, Laglaoui A, Bakkali M, Arakrak A. Isolation and screening of bacteria from rhizospheric soils of rice felds in Northwestern Morocco for different plant growth promotion (PGP) activities: An in vitro study. Int J Curr Microbiol App Sci. 2015;4(1):260-269.
30. Egamberdieva D, Berg G, Lindström K, Räsänen LA. Co-inoculation of Pseudomonas spp. with Rhizobium improves growth and symbiotic performance of fodder galega (Galega orientalis Lam.). European J Soil Biology. 2010;46:269-272.
31. Egamberdieva D, Davranov K, Wirth S, Hashem A, Abd-Allah EF. Impact of soil salinity on the plant-growth - promoting and biological control abilities of root associated bacteria. Saudi J Biological Sciences. 2017;24:1601-1608.
32. Nelson EB. Microbial dynamics and interactions in the spermosphere. Annu Rev Phytopathol. 2004;42:271-309. doi: 10.1146/annurev.phyto.42.121603.131041
33. Koshkin EI. Fiziologiya ustoychivosti sel’skokhozyaystvennykh kul’tur [Resistance physiology of agricultural crops]. Moscow: Drofa Publ.; 2010. 638 p. In Russian
34. Savich IM. Peroksidazy - stressovye belki rasteniy [Peroxidase are stress proteins of plants]. Uspekhi sovremennoy biologii = Biology Bulletin Reviews. 1989;107:406-417. In Russian
Tomsk State University Journal of Biology. 2019; : 128-141
Effect of bacterization with Aeromonas media GS4 and Pseudomonas extremorientalis PhS1 on wheat seedlings under different abiotic conditions
https://doi.org/10.17223/19988591/45/7Abstract
We studied the effect of soft wheat seed treatment (Triticum aestivum L.) with two bacterial strains (Aeromonas media GS4 and Pseudomonas extremorientalis PhS1) isolated from earthworm coprolites on the growth and development of wheat seedlings in a 12-day laboratory experiment, as well as on root rot disease and the activity of guaiacoldependant peroxidase under optimal conditions and abiotic stress (elevated and low temperatures and moisture content). We established that growing nonbacterized wheat plants under stress abiotic conditions reduced the height of plants compared to growing under optimal abiotic conditions, and seed bacterization with P. extremorientalis PhS1 strain increased wheat plant height (by 9-15%) under stress abiotic conditions compared to the nonbacterized plants. Bacterization with both strains decreased infestation of wheat seedlings (2.5-4 times) by root rots under unfavorable abiotic conditions compared to nonbacterized plants. In addition, under optimal and arid conditions, bacterization with P. extremorientalis PhS1 strain was the most effective, and under humid conditions it was bacterization with A. media GS4 strain. We showed that the activity of guaiacoldependant peroxidase correlates with the development of plant resistance to abiotic stress. In our experiments, plant bacterization resulted in a 2-fold increase in peroxidase activity both in leaves and roots of wheat plants compared to the nonbacterized plants. As the result, the ability of bacteria to activate peroxidase can serve as an information indicator of strengthening protective mechanisms of plants during bacterization. The paper contains 4 Figures and 34 References.
References
1. Gontia-Mishra I, Sapre S, Sharma A, Tiwari S. Amelioration of drought tolerance in wheat by the interaction of plant growth-promoting rhizobacteria. Plant Biology. 2016;18(6):992- 1000. doi: 10.1111/plb.12505
2. Choudhary DK, Kasotia A, Jain S, Vaishnav A, Kumari S, Sharma KP, Varma A. Bacterialmediated tolerance and resistance to plants under abiotic and biotic stresses. J Plant Growth Regul. 2016;35:276-300. doi 10.1007/s00344-015-9521-x
3. Kumar A, Verma JP. Does plant-microbe interaction confer stress tolerance in plants: A review? Microbiological Research. 2018;207:41-52. doi: 10.1016/j.micres.2017.11.004
4. Javadian N, Karimzadeh G, Mahfoozi S, Ghanati F. Cold induced changes of enzymes, proline, carbohydrates, and chlorophyll in wheat. Russ J Plant Physiol. 2010;57(4):540-547.
5. Kasim WA, Osman ME, Omar MN, Abd El-Daim IA, Bejai S, Meijer J. Control of drought stress in wheat using plant-growth-promoting bacteria. J Plant Growth Regul. 2013;32:122- 130. doi: 10.1007/s00344-012-9283-7
6. Khalilzadeh R, Sharif RS, Jalilian J. Antioxidant status and physiological responses of wheat (Triticum aestivum L.) to cycocel application and bio fertilizers under water limitation condition. J Plant Interactions. 2016;11(1):130-137. doi: 10.1080/17429145.2016.1221150
7. Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. 2010;48(12):909-930. doi: 10.1016/j.plaphy.2010.08.016
8. Kreslavski VD, Los DA, Allakhverdiev SI, Kuznetsov VlV. Signaling role of reactive oxygen species in plants under stress. Russ J Plant Physiol. 2012;59(2):141-154. doi: 10.1134/S1021443712020057
9. Perez IB, Brown PJ. The role of ROS signaling in cross-tolerance: From model to crop. Front Plant Sci. 2014;5:754-. doi: 10.3389/fpls.2014.00754
10. Baxter A, Mittler R, Suzuki N. ROS as key players in plant stress signalling. J Experimental Botany. 2013;65(5):1229-40. doi: 10.1093/jxb/ert375
11. Shalygo NV, Domanskaya IN, Radyuk MS, Shcherbakov RA, Dremuk IA. Accumulation of hydrogen peroxide and functioning of defense system in overwatered barley seedlings. Russ J Plant Physiol. 2012;59(6);748-756. doi: 10.1134/S1021443712050147
12. Maksimov IV, Cherepanova EA, Burkhanova GF, Sorokan AV, Kuz’mina OI. Structural and functional features of plant isoperoxidases. Biochemistry (Moscow). 2011;76(6):609- 621. doi: 10.1134/S0006297911060010
13. Dimkpa C, Weinand T, Asch F. Plant-rhizobacteria interactions alleviate abiotic stress conditions. Plant, Cell and Environment. 2009;32:1682-1694. doi: 10.1111/j.1365- 3040.2009.02028.x
14. de Souza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology. 2015;38(4):401-419. doi: 10.1590/S1415-475738420150053
15. Maksimov IV, Veselova SV, Nuzhnaya TV, Sarvarova ER, Khairullin RM. Plant growth promoting bacteria in regulation of plant resistance to stress factors. Russ J Plant Physiol. 2015;62:715-726.
16. Minaeva OM, Akimova EE, Tereshchenko NN, Zyubanova TI, Apenysheva MV, Kravets AV. Effect ofPseudomonasbacteria on peroxidase activity in wheat plants when infected withBipolaris sorokiniana. Russ J Plant Physiol. 2018;65(5):366-375. doi: 10.1134/S1021443718040052
17. Sukkasem P, Kurniawan A, Kao T-C, Chuang H-W. A multifaceted rhizobacterium Bacillus licheniformis functions as a fungal antagonist and a promoter of plant growth and abiotic stress tolerance. Environmental and Experimental Botany. 2018;155:541-551. doi: 10.1016/j.envexpbot.2018.08.005
18. Abd El-Daim IA, Bejai S, Fridborg I, Meijer J. Identifying potential molecular factors involved in Bacillus amyloliquefaciens 5113 mediated abiotic stress tolerance in wheat. Plant Biology. 2018;20(2):271-279. doi: 10.1111/plb.12680
19. Tereshchenko N, Akimova E, Minaeva O, Kravets A, Zyubanova T. Presowing with bacteria improved the productivity and resistance to fungal root pathogen in wheat and barley. In: Grasses as Food and Feed. Tadele Z, editor. London, United Kingdom: IntechOpen Publ.; 2018. pp. 153-167. doi: 10.5772/intechopen.80084
20. Minaeva OM, Akimova EE, Tereshchenko NN, Kravets AV, Zyubanova TI, Apenysheva MV. Pseudomonads associated with soil lumbricides as promising agents in root rod biocontrol for spring grain crops. Sel’skokhozyaistvennaya biologiya = Agricultural Biology. 2019;54(1):91-100. doi: 10.15389/agrobiology.2019.1.91rus In Russian, English Summary
21. Minaeva OM, Akimova EE. Effectiveness of applying bacteria Pseudomonas sp., strain B-6798, for anti-phytopathogenic protection of crops in Western Siberia. Vestnik Tomskogo Gosudarstvennogo Universiteta. Biologiya = Tomsk State University Journal of Biology. 2013;3(23):19-37.
22. Baslavskaya SS, Trubetskova OM. Praktikum po fziologii rasteniy [Practical Works on Plant Physiology]. Moscow: Moscow State Univ. Publ.; 1964. 326 r. In Russian
23. Cooke BM. Disease assessment and yield loss. In: The Epidemiology of Plant Diseases. Cooke BM, Jone DG, Kaye B, editors. 2nd ed. Dorchert: Springer Publ.; 2006. pp. 43- 80.
24. Chupakhina GN. Fiziologicheskie i biokhimicheskie metody analiza rasteniy: Praktikum [Physiological and biochemical methods of plant analysis: Workshop]. Kaliningrad: Kaliningrad Univ. Publ.; 2000. 59 p. In Russian
25. Chance B, Maehly AC. Assays of catalases and peroxidases. In: Methods in Enzymology. Colowick SP and Kaplan NO, editors. New York: Academic Press; 1955. Vol II. pp. 764- 775.
26. Kovalev VM. Teoriya urozhaya [Harvesting theory]. 3rd ed. Moscow: Moscow Agricultural Academy Publ.; 2003. 332 p. In Russian
27. Acevedo E, Silva P, Silva H. Wheat growth and physiology. In: Bread wheat Improvement and production. Curtis BC, Rajaram S and Gomez Macpherson H, editors. Rome: FAO; 2002. pp. 39-70. Available at: http://www.fao.org/3/Y4011E/y4011e00.htm#Contents (access 15.01.2019).
28. Arraktham S, Tancho A, Niamsup P, Rattanawaree P. The potential of bacteria isolated from earthworm intestines, vermicompost and liquid vermicompost to produce indole-3-acetic acid (IAA). J Agricultural Technology. 2016;12(2):229-239.
29. Aarab S, Ollero FJ, Megías M, Laglaoui A, Bakkali M, Arakrak A. Isolation and screening of bacteria from rhizospheric soils of rice felds in Northwestern Morocco for different plant growth promotion (PGP) activities: An in vitro study. Int J Curr Microbiol App Sci. 2015;4(1):260-269.
30. Egamberdieva D, Berg G, Lindström K, Räsänen LA. Co-inoculation of Pseudomonas spp. with Rhizobium improves growth and symbiotic performance of fodder galega (Galega orientalis Lam.). European J Soil Biology. 2010;46:269-272.
31. Egamberdieva D, Davranov K, Wirth S, Hashem A, Abd-Allah EF. Impact of soil salinity on the plant-growth - promoting and biological control abilities of root associated bacteria. Saudi J Biological Sciences. 2017;24:1601-1608.
32. Nelson EB. Microbial dynamics and interactions in the spermosphere. Annu Rev Phytopathol. 2004;42:271-309. doi: 10.1146/annurev.phyto.42.121603.131041
33. Koshkin EI. Fiziologiya ustoychivosti sel’skokhozyaystvennykh kul’tur [Resistance physiology of agricultural crops]. Moscow: Drofa Publ.; 2010. 638 p. In Russian
34. Savich IM. Peroksidazy - stressovye belki rasteniy [Peroxidase are stress proteins of plants]. Uspekhi sovremennoy biologii = Biology Bulletin Reviews. 1989;107:406-417. In Russian
