Рецепт. 2019; 22: 818-831
Определение безопасности водных извлечений из травы галеги лекарственной
Аннотация
Цель работы: доклинические токсикологические испытания водного извлечения из травы галеги лекарственной.
Материалы и методы: объектом исследования служило водное извлечение из травы галеги лекарственной, а именно отвар в соотношении сырье – экстрагент 1:10. Изучение общетоксического действия в подостром эксперименте извлечений из травы галеги лекарственной проводили при многократном (ежедневном) пероральном введении крысам максимальных переносимых доз в 28-дневном эксперименте. Статистическую обработку результатов проводили при помощи программы Statistica 7.0. Проведенное исследование показало, что водное извлечение из травы галеги лекарственной при интрагастральном введении в течение 28 суток крысам в дозе 800,0 мг/кг не оказывает токсического действия на организм животных. Макроскопическое исследование внутренних органов не выявило патологических изменений. Не обнаружено признаков гематоксичности, нефротоксичности, органной токсичности по результатам физиологических тестов, данным общего анализа крови и мочи, биохимического анализа крови, также не выявлено влияния на уровни кортизола и тестостерона у крыс-самцов. В ходе исследований выявлен антиатерогенный эффект извлечения из травы галеги лекарственной.
Список литературы
1. Godovalnikov G. (2008) Modern Pharmacology. Brest: Brest Printing House OJSC, 520 p.
2. Sheryakova A. (ed.) (2012) General methods of drug control. Infusion, decoctions and teas. The State Pharmacopoeia of the Republic of Belarus (State Pharmacopoeia of Belarus II): in 2 volumes. RUE Center for Expertise and Nutrition in Health Care. Molodechno: Pobeda, pp. 1072–1074.
3. Belenky M. (1963) Elements of a quantitative assessment of the pharmacological effect. L: State publishing house honey. literature, 152 p.
4. Mironova A. (ed.) (2013) Guidelines for preclinical studies of drugs. Part One. M.: Grif and K, 944 p.
5. Glanz S. (1999) Biomedical statistics. Moscow: Practice, 459 p.
6. (2006) Sanitary rules and norms 2.1.2.12-18-2006 “Design, equipment and maintenance of experimental biological clinics (vivariums)”. Enter. 01/02/07. Minsk, 21 p.
7. (2008) Technical Code of Good Practice 125-2008 (02040) Good Laboratory Practice: Appendix to Decree of the Ministry of Health of the Republic of Belarus. March 28, No 56.
8. Khabriev R. (ed.) (2005) Guidance on the experimental (preclinical) study of new substances. RAMS, 2nd ed., Rev. and Dop. M.: Medicine, 832 p.
9. (2004) Requirements for the setting up of experimental studies for the initial toxicological assessment and hygienic regulation of substances. Instruction 1.1.11-12-35-2004: approved the main state Health Inspector Rep. Belarus 12/14/2004. Minsk, 44 p.
10. Nozdrachev A., Polyakov E. (2001) Anatomy of a rat. St. Petersburg: Doe, 464 p.
11. Kuznetsov S., Mushkambarov N., Goryachkina V. (2002) Atlas on histology, cytology and embryology. M.: Medical News Agency, 374 p.
Recipe. 2019; 22: 818-831
Determination of the Safety of Water Extracts from the Herb Galega Officinalis
Abstract
The purpose of the work is preclinical toxicological testing of water extraction from the Galega officinalis L. herb.
Materials and methods: the object of the research was the water extraction of the Galega officinalis L. herb, namely a decoction in the ratio of raw material – extractant 1:10. The research of the general toxic effect in the subacute experiment of water extraction of the Galega officinalis L. herb was carried out with repeated (daily) oral administration to rats of the maximum tolerated doses in a 28- day experiment. Statistical processing of the results was carried out using the Statistica 7.0 program. The conducted research showed that the water extraction of the Galega officinalis L. herb with intragastric administration within 28 days to rats at a dose of 800.0 mg/kg does not have a toxic effect on the animal organism. A macroscopic examination of the internal organs revealed no pathological changes. There were no signs of hematoxicity, nephrotoxicity, organ toxicity according to the results of physiological tests, data from a general analysis of blood and urine, a biochemical analysis of blood, nor was there an effect on the levels of cortisol and testosterone in male rats. In the course of research the anti-atherogenic effect of extraction of the Galega officinalis L. herb was identified.
References
1. Godovalnikov G. (2008) Modern Pharmacology. Brest: Brest Printing House OJSC, 520 p.
2. Sheryakova A. (ed.) (2012) General methods of drug control. Infusion, decoctions and teas. The State Pharmacopoeia of the Republic of Belarus (State Pharmacopoeia of Belarus II): in 2 volumes. RUE Center for Expertise and Nutrition in Health Care. Molodechno: Pobeda, pp. 1072–1074.
3. Belenky M. (1963) Elements of a quantitative assessment of the pharmacological effect. L: State publishing house honey. literature, 152 p.
4. Mironova A. (ed.) (2013) Guidelines for preclinical studies of drugs. Part One. M.: Grif and K, 944 p.
5. Glanz S. (1999) Biomedical statistics. Moscow: Practice, 459 p.
6. (2006) Sanitary rules and norms 2.1.2.12-18-2006 “Design, equipment and maintenance of experimental biological clinics (vivariums)”. Enter. 01/02/07. Minsk, 21 p.
7. (2008) Technical Code of Good Practice 125-2008 (02040) Good Laboratory Practice: Appendix to Decree of the Ministry of Health of the Republic of Belarus. March 28, No 56.
8. Khabriev R. (ed.) (2005) Guidance on the experimental (preclinical) study of new substances. RAMS, 2nd ed., Rev. and Dop. M.: Medicine, 832 p.
9. (2004) Requirements for the setting up of experimental studies for the initial toxicological assessment and hygienic regulation of substances. Instruction 1.1.11-12-35-2004: approved the main state Health Inspector Rep. Belarus 12/14/2004. Minsk, 44 p.
10. Nozdrachev A., Polyakov E. (2001) Anatomy of a rat. St. Petersburg: Doe, 464 p.
11. Kuznetsov S., Mushkambarov N., Goryachkina V. (2002) Atlas on histology, cytology and embryology. M.: Medical News Agency, 374 p.
