Рецепт. 2020; : 701-708
Молекулярно-генетические исследованияв морфологической диагностике туберкулеза
https://doi.org/10.34883/PI.2020.23.5.006Аннотация
Представлены результаты молекулярно-генетического исследования (тест Xpet MTB/Rif и метод гибридизации с ДНК-зондами) гистологического биоматериала парафиновых блоков 152 пациентов. Установлено, что параллельное выполнение гистологического исследования биопсийного/ операционного материала с молекулярно-генетическим исследованием с определением ДНК МБТ и лекарственной чувствительности M. tuberculosis в парафиновых блоках расширяет возможности морфологической диагностики туберкулеза и повышает ее достоверность на 31,74%.Показана целесообразность проведения молекулярно-генетического исследования парафиновых блоков гистологического биоматериала в диагностике туберкулеза и определении лекарственной чувствительности возбудителя непосредственно в очагах поражения.
Список литературы
1. Tsizerling V. (2015) Morfologicheskaya diagnostika tuberkuleza [Morphological diagnostics of tuberculosis]. Arkhiv patologii, 3, pp. 3–9.
2. David J., Dabbs M.D. (2014) Diagnostic immunohistochemistry (theranostic and genomic applications). 4th edition. USA, p. 64.
3. Belousova K. (2013) Kharakteristika klinicheski znachimykh biologicheskikh svoystv vozbuditelya tuberkuleza. vydelennogo iz rezetsirovannykh uchastkov legkikh bolnykh tuberkulezom [Characteristics of clinically significant biological properties of the causative agent of tuberculosis isolated from the resected parts of the lungs in patients with tuberculosis] (PhD Thesis), Ekaterinburg, 23 p.
4. Surkova L., Dziusmikeyeva M., Shpakovskaya N. (2018) Morfologicheskaya diagnostika tuberkuleza (aktualnyye voprosy, sovremennyye metody i podkhody k diagnostike). Retsept. Prilozheniye 28. Sb. nauch. trudov pervogo syezda ftiziatrov i pulmonologov Respubliki Belarus. 17–18 maya 2018 g. Minsk. Sovremennyye napravleniya razvitiya respiratornoy meditsiny i ftiziatrii [Morphological diagnostics of tuberculosis (topical issues, modern methods and approaches to diagnostics). Recipe. Supplement 28. Collection of scientific papers of the First Congress of phthisiatricians and pulmonologists of the Republic of Belarus. May 17–18. Minsk. Modern directions of development of respiratory medicine and phthisiology]. Minsk, pp. 147–150.
5. Dziusmikeyeva M., Yatskevich N., Skrahina A., Haranok D., Zhurkin D. (2018) Kompleksnyy metod uskorennoy diagnostiki mnozhestvenno lekar-stvenno-ustoychivogo tuberkuleza [Complex method of rapid diagnostics of multiple drug-resistant tuberculosis]. Aktualnyye problemy mikrobiologii. virusologii. immunologii. Mater. Yubileynoy nauchno-praktich. konf. k 100-letiyu so dnya rozhdeniya professora A.P. Krasilnikova i 95-letiyu osnovaniya kafedry mikrobiologii. virusologii. immunologii BGMU. Minsk. 19 oktyabrya 2018 [Topical issues of microbiology, virology, immunology. Materials of the Anniversary scientific-practical conference to the 100th anniversary of the birth of Professor A.R. Krasilnikov and 95th anniversary of the foundation of the Department of microbiology, virology, immunology of the BSMU. Minsk. October 19, 2018]. Minsk, pp. 55–58.
Recipe. 2020; : 701-708
Molecular-Genetic Studiesin the Morphological Diagnosis of Tuberculosis
https://doi.org/10.34883/PI.2020.23.5.006Abstract
References
1. Tsizerling V. (2015) Morfologicheskaya diagnostika tuberkuleza [Morphological diagnostics of tuberculosis]. Arkhiv patologii, 3, pp. 3–9.
2. David J., Dabbs M.D. (2014) Diagnostic immunohistochemistry (theranostic and genomic applications). 4th edition. USA, p. 64.
3. Belousova K. (2013) Kharakteristika klinicheski znachimykh biologicheskikh svoystv vozbuditelya tuberkuleza. vydelennogo iz rezetsirovannykh uchastkov legkikh bolnykh tuberkulezom [Characteristics of clinically significant biological properties of the causative agent of tuberculosis isolated from the resected parts of the lungs in patients with tuberculosis] (PhD Thesis), Ekaterinburg, 23 p.
4. Surkova L., Dziusmikeyeva M., Shpakovskaya N. (2018) Morfologicheskaya diagnostika tuberkuleza (aktualnyye voprosy, sovremennyye metody i podkhody k diagnostike). Retsept. Prilozheniye 28. Sb. nauch. trudov pervogo syezda ftiziatrov i pulmonologov Respubliki Belarus. 17–18 maya 2018 g. Minsk. Sovremennyye napravleniya razvitiya respiratornoy meditsiny i ftiziatrii [Morphological diagnostics of tuberculosis (topical issues, modern methods and approaches to diagnostics). Recipe. Supplement 28. Collection of scientific papers of the First Congress of phthisiatricians and pulmonologists of the Republic of Belarus. May 17–18. Minsk. Modern directions of development of respiratory medicine and phthisiology]. Minsk, pp. 147–150.
5. Dziusmikeyeva M., Yatskevich N., Skrahina A., Haranok D., Zhurkin D. (2018) Kompleksnyy metod uskorennoy diagnostiki mnozhestvenno lekar-stvenno-ustoychivogo tuberkuleza [Complex method of rapid diagnostics of multiple drug-resistant tuberculosis]. Aktualnyye problemy mikrobiologii. virusologii. immunologii. Mater. Yubileynoy nauchno-praktich. konf. k 100-letiyu so dnya rozhdeniya professora A.P. Krasilnikova i 95-letiyu osnovaniya kafedry mikrobiologii. virusologii. immunologii BGMU. Minsk. 19 oktyabrya 2018 [Topical issues of microbiology, virology, immunology. Materials of the Anniversary scientific-practical conference to the 100th anniversary of the birth of Professor A.R. Krasilnikov and 95th anniversary of the foundation of the Department of microbiology, virology, immunology of the BSMU. Minsk. October 19, 2018]. Minsk, pp. 55–58.
