Альманах клинической медицины. 2014; 1: 21-24
ЭКСПЕРИМЕНТАЛЬНАЯ МОДЕЛЬ ПЕРВИЧНОГО МУЖСКОГО ГИПОГОНАДИЗМА
https://doi.org/10.18786/2072-0505-2014-31-21-24Аннотация
Актуальность. Разработка новых методов лечения первичного мужского гипогонадизма – актуальная медицинская проблема, для решения которой необходима удобная экспериментальная модель заболевания.
Цель – создание экспериментальной модели первичного мужского гипогонадизма.
Материал и методы. Исследование проводили на крысах-самцах Wistar, гипогонадизм инициировали путем временного наложения лигатуры на дистальную часть семенного канатика.
Основные результаты. Лигирование семенного канатика в течение трех суток приводит к стойкому нарушению репродуктивной и гормонообразующих функций. При меньших сроках воздействия нарушения самопроизвольно обратимы. Заключение. Созданная модель первичного мужского гипогонадизма характеризуется стойким нарушением гормонообразующей и репродуктивной функций, технической доступностью, отсутствием токсического эффекта на другие органы и системы. Наличие модели открывает новые возможности для разработки подходов к лечению заболеваний репродуктивных органов у мужчин.
Список литературы
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2. Dohle G.R., Arver S., Bettocchi C., Kliesch S., Punab M., de Ronde W. Guidelines on male hypogonadism. Arnhem (The Netherlands): European Association of Urology; 2012.
3. Huhtaniemi I. Late-onset hypogonadism: current concepts and controversies of pathogenesis, diagnosis and treatment. Asian J Androl 2014;16(2):192-202.
4. Corona G., Rastrelli G., Monami M., Guay A., Buvat J., Sforza A., Forti G., Mannucci E., Maggi M. Hypogonadism as a risk factor for cardiovascular mortality in men: a meta-analytic study. Eur J Endocrinol 2011;165(5):687-701.
5. Kliesch S. Testosterone and infertility. Urologe A 2010; 49(1):32-6.
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11. Yuluğ E., Türedi S., Karagüzel E., Kutlu O., Menteşe A., Alver A. The short term effects of resveratrol on ischemia-reperfusion injury in rat testis. J Pediatr Surg 2014;49(3):484-9.
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Almanac of Clinical Medicine. 2014; 1: 21-24
EXPERIMENTAL MODEL OF THE PRIMARY MALE HYPOGONADISM
https://doi.org/10.18786/2072-0505-2014-31-21-24Abstract
Background: Development of the new methods of treatment of primary male hypogonadism is an urgent medical problem. Its solution requires a suitable experimental model of the disease. Aim: The creation of new experimental model of primary male hypogonadism. Materials and methods: The study was conducted on the male Wistar rats, hypogonadism was modeled by temporary ligation of the distal part of the spermatic cord. Results: It was shown that three-day ligation of the spermatic cord led to persistent disturbance of the testosterone-producing and reproductive functions. These manifestations were reversible at shorter duration of the exposure. Conclusion: The created model of primary male hypogonadism is characterized by the persistent testosterone-producing and reproductive functions disturbance, technical availability, non-toxicity to the other organs and systems. Availability of the model provides new opportunities for the development of approaches to treating diseases of the reproductive organs in men.
References
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2. Dohle G.R., Arver S., Bettocchi C., Kliesch S., Punab M., de Ronde W. Guidelines on male hypogonadism. Arnhem (The Netherlands): European Association of Urology; 2012.
3. Huhtaniemi I. Late-onset hypogonadism: current concepts and controversies of pathogenesis, diagnosis and treatment. Asian J Androl 2014;16(2):192-202.
4. Corona G., Rastrelli G., Monami M., Guay A., Buvat J., Sforza A., Forti G., Mannucci E., Maggi M. Hypogonadism as a risk factor for cardiovascular mortality in men: a meta-analytic study. Eur J Endocrinol 2011;165(5):687-701.
5. Kliesch S. Testosterone and infertility. Urologe A 2010; 49(1):32-6.
6. Jungwirth A., Diemer T., Dohle G.R., Giwercman A., Kopa Z., Tournaye H., Krausz C. Guidelines on male infertility. Arnhem (The Netherlands): European Association of Urology; 2013.
7. Sukhikh G.T., Kamalov A.A., Poltavtseva R.A., Zaraiskii E.I., Plotnikov E.Y., Kirpatovskii V.I., Efremov E.A., Orlova E.V., Pavlova G.V., Okhobotov D.A. Effect of xenotransplantation of cell cultures enriched with stem and progenitor cells on hormonal profile of rats with abdominal cryptorchism. Bull Exp Biol Med 2008;146(4):517-21.
8. Zhang Z., Shao S., Meistrich M.L. Irradiated mouse testes efficiently support spermatogenesis derived from donor germ cells of mice and rats. J Androl 2006;27(3):365-75.
9. Meistrich M.L. Male gonadal toxicity. Pediatr Blood Cancer 2009;53(2):261-6.
10. Swerdloff R.S., Lue Y., Liu P.Y., Erkkilä K., Wang C. Mouse model for men with klinefelter syndrome: a multifaceted fit for a complex disorder. Acta Paediatr 2011;100(6):892-9.
11. Yuluğ E., Türedi S., Karagüzel E., Kutlu O., Menteşe A., Alver A. The short term effects of resveratrol on ischemia-reperfusion injury in rat testis. J Pediatr Surg 2014;49(3):484-9.
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