Офтальмохирургия. 2020; : 40-44
Фактор роста нервов у пациентов с возрастной катарактой
https://doi.org/10.25276/0235-4160-2020-3-40-44Аннотация
Цель. Изучить содержание фактора роста нервов (ФРН) в слезной жидкости (СЖ), влаге передней камеры (ВПК) и сыворотке крови (СК) пациентов с возрастной катарактой.
Материал и методы. Изучено содержание ФРН в СЖ, ВПК и СК у 47 пациентов (47 глаз) с возрастной катарактой. Забор стимулированной СЖ проводили пипеточным дозатором в день перед операцией, ВПК и СК – во время операции факоэмульсификации катаракты с имплантацией интраокулярной линзы. Концентрацию ФРН в указанных биологических жидкостях определяли методом иммуноферментного анализа.
Результаты. Концентрация ФРН во ВПК составила 27,6±12,8 (2,7–56,1) пг/мл, в СК – 23,2±8,9 (5,9–41,6) пг/мл, в СЖ – 85,3±23,3 (36,6–135,9) пг/мл. Уровень ФРН во ВПК достоверно коррелировал только с его содержанием в СЖ: коэффициент корреляции Пирсона – r=0,526, p<0,000, корреляция с его концентрацией в СК была недостоверной.
Заключение. У пациентов с возрастной катарактой наиболее высокий уровень ФРН определяется в СЖ, концентрации его в СК и ВПК более чем в 3 раза ниже. Содержание ФРН во ВПК значимо коррелирует только с его концентрацией в СЖ.
Список литературы
1. Levi-Montalcini R. The nerve growth factor 35 years later. Science. 1987;237: 1154–1162. doi:10.1126/science.3306916
2. Sampaio TB, Savall AS, Gutierrez MEZ, Pinton S. Neurotrophic factors in Alzheimer’s and Parkinson’s diseases: implications for pathogenesis and therapy. Neural Regen Res. 2017;12(4): 549–557. doi:10.4103/1673-5374.205084
3. Cuello AC, Bruno MA, Allard S, Leon W, Iulita MF. Cholinergic involvement in Alzheimer’s disease. A link with NGF maturation and degradation. J. Mol. Neurosci. 2010;40(1-2): 230–235. doi:10.1007/s12031-009-9238-z
4. Neugebauer K, Hammans C, Wensing T, Kumar V, Grodd W, Mevissen L, Sternkopf MA, Novakovic A, Abel T, Habel U, Nickl-Jockschat T. Nerve growth factor serum levels are associated with regional gray matter volume differences in schizophrenia patients. Front Psychiatry. 2019;10: 275. doi:10.3389/fpsyt.2019.00275
5. Chen YW, Lin PE, Tu KY, Cheng YS, Wu CK, Tseng PT. Significantly lower nerve growth factor levels in patients with major depressive disorder then in healthy subjects: a meta-analysis and systematic review. Neuropsychiatr Dis Treat. 2015;11: 925–933. doi:10.2147/NDT.S81432
6. Lambiase A, Micera A, Sacchetti M, Cortes M, Mantelli F, Bonini S. Alterations of tear neuromediators in dry eye disease. Arch Ophthalmol. 2011;129(8): 981–986. doi:10.1001/archophthalmol.2011.200
7. Cortes M, Esposito G, Sacco R, Gillet VB, Ianni A, Micera A. NGF and iNOS changes in tears from video display terminal workers. Curr Eye Res. 2018;43(9): 1119–1125. doi:10.1080/02713683.2018.1475014
8. Murugesan V, Dwivedi R, Saini M, Gupta V, Dada T, Vivekanandhan S. Tear neuromediators in eyes on chronic topical antiglaucoma therapy with and without BAK preservatives. Br J Ophthalmol. 2019. doi:10.1136/bjophthalmol-2019-314234
9. Chowdhury UR, Madden BJ, Charlesworth MC, Fautsch MP. Proteome analysis of human aqueous humor. Invest Ophthalmol Vis Sci. 2010;51(10): 4921–4931. doi:10.1167/iovs.10-5531
10. Chalam KV, Sharma RK, Murthy RK. Limitations in assessing nerve growth factor levels in aqueous humor samples from human eyes. BMC Res Notes. 2008;1:22. doi:10.1186/1756-0500-1-22
11. Cheng Y, Feng J, Zhu X, Liang J. Cytokines concentrations in aqueous humor of eyes with uveal melanoma. Medicine. 2019;98(5): e14030. doi:10.1097/MD.0000000000014030
12. Thylefors B, Chylack LT, Konyama K, Sasaki K, Sperduto R, Taylor HR, West S, WHO Cataract Grading Group. A simplified cataract grading system. Ophthalmic Epidemiol. 2002;9(2): 83–95. doi:10.1076/opep.9.2.83.1523
13. Гомазков О.А. Старение мозга и нейротрофическая терапия. М.: ИКАР; 2011. [Gomazkov OA. Stareniye mozga i neirotroficheskaya terapiya. Moskva: IKAR; 2011. (In Russ.)]
14. Lewin GR, Barde YA. Physiology of the neurotrophins. Ann Rev Neurosci. 1996;19: 289–317. doi:10.1146/annurev.ne.19.030196.001445
15. Bonini S, Aloe L, Rama P, Lamagna A, Lambiase A. Nerve growth factor (NGF): an important molecule for trophism and healing of the ocular surface. Adv Exp Med Biol. 2002;506: 531–537.
16. Lambiase A, Rama P, Bonini S, Caprioglio G, Aloe L. Topical treatment with nerve growth factor for corneal neurotrophic ulcers. N Engl J Med. 1998;338(17): 1174–1180. doi:10.1056/NEJM199804233381702
17. Di Girolamo N, Sarris M, Chui J, Cheema H, Coroneo MT, Wakefield D. Localization of the lowaffinity nerve growth factor receptor p75 in human limbal epithelial cells. J Cell Mol Med. 2008;12(6B): 2799–2811. doi:10.1111/j.1582-4934.2008.00290.x
18. Lambiase A, Bonini S, Manni L, Ghinelli E, Tirassa P, Rama P, Aloe L. Intraocular production and release of nerve growth factor after iridectomy. Invest Ophthalmol Vis Sci. 2002;43(7): 2334–2340.
19. Ghinelli E, Aloe L, Cortes M, Micera A, Lambiase A, Bonini S. Nerve growth factor (NGF) and lenses: effects of NGF in an in vitro rat model of cataract. Graefes Arch Clin Exp Ophthalmol. 2003;241(10): 845–851. doi:10.1007/s00417-003-0733-6
20. Muzi S, Colafrancesco V, Sornelli F, Mantelli F, Lambiase A, Aloe L. Nerve growth factor in the developing and adult lacrimal glands of rat with and without inherited retinitis pigmentosa. Cornea. 2010;29(10): 1163–1168. doi:10.1097/ICO.0b013e3181d3d3f9
21. Lambiase A, Bonini S, Micera A, Rama P, Bonini S, Aloe L. Expression of nerve growth factor receptors on the ocular surface in healthy subjects and during manifestation of inflammatory diseases. Invest Ophthalmol Vis Sci. 1998;39(7): 1272–1275.
22. Hah YS, Yoo WS, Seo S, Chung I, Kim HA, Cho HY, Kim SJ. Reduced NGF level promotes epithelial mesenchymal transition in human lens epithelial cells exposed to high dexamethasone concentrations. Curr Eye Res. 2019. doi:10.1080/02713683.2019.1695844
23. Lee HK, Lee KS, Kim HC, Lee SH, Kim EK. Nerve growth factor concentration and implications in photorefractive keratectomy vs laser in situ keratomileusis. Am J Ophthalmol. 2005;139(6): 965–971. doi:10.1016/j.ajo.2004.12.051
24. Micera A, Lambiase A, Aloe L, Bonini S, Levi- Schaffer F, Bonini S. Nerve growth factor involvement in the visual system: implications in allergic and neurodegenerative diseases. Cytokine Growth Factor Rev. 2004;15(6): 411–417. doi:10.1016/j.cytogfr.2004.09.003
25. Roberti G, Mantelli F, Macchi I, Massaro- Giordano M, Centofanti M. Nerve growth factor modulation of retinal ganglion cell physiology. J Cell Physiol. 2014;229: 1130–1133. doi:10.1002/jcp.24573
26. Amendola T, Fiore M, Aloe L. Postnatal changes in nerve growth factor and brain derived neurotrophic factor levels in the retina, visual cortex, and geniculate nucleus in rats with retinitis pigmentosa. Neurosci Lett. 2003;345(1): 37–40. doi:10.1016/s0304-3940(03)00491-9
27. Pizzorusso T, Fagiolini M, Gianfranceschi L, Porciatti V, Maffei L. Role of neurotrophins in the development and plasticity of the visual system: experiments on dark rearing. Int J Psychophysiol. 2000;35(2-3): 189–196. doi:10.1016/s0167-8760(99)00053-7
28. Rocco ML, Soligo M, Manni L, Aloe L. Nerve growth factor: early studies and recent clinical trials. Curr Neuropharmacol. 2018;16: 1455–1465. doi:10.2174/1570159X16666180412092859
29. Oddone F, Roberti G, Micera A, Busanello A, Bonini S, Quaranta L, Agnifili L, Manni G. Exploring serum levels of brain derived neurotrophic factor and nerve growth factor across glaucoma stages. PLoS One. 2017;12: e0168565. doi:10.1371/journal.pone.0168565
30. Park KS, Kim SS, Kim JC, Kim HC, Im YS, Anh CW, Lee HK. Serum and tear levels of nerve growth factor in diabetic retinopathy patients. Am J Ophthalmol. 2008;145(3): 432–437. doi:10.1016/j.ajo.2007.11.011
31. Xu JB, Yang XC, Guo JH, Chen JY, Shang Y, Liu WJ, Wang TM, Zou L. Serum nerve growth factor level indicates therapeutic efficacy of 125I seed implantation in advanced pancreatic adenocarcinoma. Eur Rev Med Pharmacol Sci. 2015;19(18): 3385–3390.
32. Carmignoto G, Maffei L, Candeo P, Canella R, Comelli C. Effect of NGF on the survival of rat retinal ganglion cells following optic nerve section. J Neurosci.1989;9(4): 1263–1272.
33. Шпак А.А., Гехт А.Б., Дружкова Т.А., Козлова К.И., Гуляева Н.В. Нейротрофические факторы у пациентов с первичной открытоугольной глаукомой и возрастной катарактой. Сообщение 1. Цилиарный нейротрофический фактор. Офтальмохирургия. 2018;3: 41–45. [Shpak AA, Guekht AB, Druzhkova TA, Kozlova KI, Gulyaeva NV. Neurotrophic factors in patients with primary open-angle glaucoma and agerelated cataract. Part 1. Ciliary neurotrophic factor. Fyodorov Journal of Ophthalmic Surgery. 2018;3: 41–45. (In Russ.)] doi:10.25276/0235-4160-2018-3-41-45.
34. Шпак А.А., Гехт А.Б., Дружкова Т.А., Козлова К.И., Гуляева Н.В. Нейротрофические факторы у пациентов с первичной открытоугольной глаукомой и возрастной катарактой. Сообщение 2. Нейротрофический фактор головного мозга. Офтальмохирургия. 2018;4: 46–51. [Shpak AA, Guekht AB, Druzhkova TA, Kozlova KI, Gulyaeva NV. Neurotrophic factors in patients with primary open-angle glaucoma and age-related cataract. Part 2. Brain-derived neurotrophic factor. Fyodorov Journal of Ophthalmic Surgery. 2018;4: 46–51. (In Russ.)] doi:10.25276/0235-4160-2018-4-46-51
Fyodorov Journal of Ophthalmic Surgery. 2020; : 40-44
Nerve growth factor in patients with age-related cataract
https://doi.org/10.25276/0235-4160-2020-3-40-44Abstract
Purpose. To study the content of nerve growth factor (NGF) in the aqueous humor (AH), lacrimal fluid (LF), and blood serum (BS) in patients with age-related cataract.
Material and methods. The content of NGF in the LF, AH and BS was studied in 47 patients (47 eyes) with age-related cataract. Collection of stimulated LF was performed with a pipette on the day preceding surgery; the AH and BS were sampled during the phacoemulsification of a cataract. The concentration of NGF was measured using an enzyme immunoassay.
Results. The concentration of NGF in the AH was 27,6±12,8 (range 2,7–56,1) pg/ml, in the BS – 23,2±8,9 (5,9–41,6) pg/ml, in the LF – 85,3±23,3 (36,6–135,9) pg/ml. The concentration of NGF in the AH correlated well with its content in LF: Pearson’s correlation coefficient r was 0,526, p<0,000. The correlation of NGF concentrations in BS and AH was insignificant.
Conclusion. In patients with age-related cataract, the highest level of NGF was detected in the LF, its concentration in AH and BS was more than three times lower. The NGF content in the AH significantly correlated only with its concentration in LF.
References
1. Levi-Montalcini R. The nerve growth factor 35 years later. Science. 1987;237: 1154–1162. doi:10.1126/science.3306916
2. Sampaio TB, Savall AS, Gutierrez MEZ, Pinton S. Neurotrophic factors in Alzheimer’s and Parkinson’s diseases: implications for pathogenesis and therapy. Neural Regen Res. 2017;12(4): 549–557. doi:10.4103/1673-5374.205084
3. Cuello AC, Bruno MA, Allard S, Leon W, Iulita MF. Cholinergic involvement in Alzheimer’s disease. A link with NGF maturation and degradation. J. Mol. Neurosci. 2010;40(1-2): 230–235. doi:10.1007/s12031-009-9238-z
4. Neugebauer K, Hammans C, Wensing T, Kumar V, Grodd W, Mevissen L, Sternkopf MA, Novakovic A, Abel T, Habel U, Nickl-Jockschat T. Nerve growth factor serum levels are associated with regional gray matter volume differences in schizophrenia patients. Front Psychiatry. 2019;10: 275. doi:10.3389/fpsyt.2019.00275
5. Chen YW, Lin PE, Tu KY, Cheng YS, Wu CK, Tseng PT. Significantly lower nerve growth factor levels in patients with major depressive disorder then in healthy subjects: a meta-analysis and systematic review. Neuropsychiatr Dis Treat. 2015;11: 925–933. doi:10.2147/NDT.S81432
6. Lambiase A, Micera A, Sacchetti M, Cortes M, Mantelli F, Bonini S. Alterations of tear neuromediators in dry eye disease. Arch Ophthalmol. 2011;129(8): 981–986. doi:10.1001/archophthalmol.2011.200
7. Cortes M, Esposito G, Sacco R, Gillet VB, Ianni A, Micera A. NGF and iNOS changes in tears from video display terminal workers. Curr Eye Res. 2018;43(9): 1119–1125. doi:10.1080/02713683.2018.1475014
8. Murugesan V, Dwivedi R, Saini M, Gupta V, Dada T, Vivekanandhan S. Tear neuromediators in eyes on chronic topical antiglaucoma therapy with and without BAK preservatives. Br J Ophthalmol. 2019. doi:10.1136/bjophthalmol-2019-314234
9. Chowdhury UR, Madden BJ, Charlesworth MC, Fautsch MP. Proteome analysis of human aqueous humor. Invest Ophthalmol Vis Sci. 2010;51(10): 4921–4931. doi:10.1167/iovs.10-5531
10. Chalam KV, Sharma RK, Murthy RK. Limitations in assessing nerve growth factor levels in aqueous humor samples from human eyes. BMC Res Notes. 2008;1:22. doi:10.1186/1756-0500-1-22
11. Cheng Y, Feng J, Zhu X, Liang J. Cytokines concentrations in aqueous humor of eyes with uveal melanoma. Medicine. 2019;98(5): e14030. doi:10.1097/MD.0000000000014030
12. Thylefors B, Chylack LT, Konyama K, Sasaki K, Sperduto R, Taylor HR, West S, WHO Cataract Grading Group. A simplified cataract grading system. Ophthalmic Epidemiol. 2002;9(2): 83–95. doi:10.1076/opep.9.2.83.1523
13. Gomazkov O.A. Starenie mozga i neirotroficheskaya terapiya. M.: IKAR; 2011. [Gomazkov OA. Stareniye mozga i neirotroficheskaya terapiya. Moskva: IKAR; 2011. (In Russ.)]
14. Lewin GR, Barde YA. Physiology of the neurotrophins. Ann Rev Neurosci. 1996;19: 289–317. doi:10.1146/annurev.ne.19.030196.001445
15. Bonini S, Aloe L, Rama P, Lamagna A, Lambiase A. Nerve growth factor (NGF): an important molecule for trophism and healing of the ocular surface. Adv Exp Med Biol. 2002;506: 531–537.
16. Lambiase A, Rama P, Bonini S, Caprioglio G, Aloe L. Topical treatment with nerve growth factor for corneal neurotrophic ulcers. N Engl J Med. 1998;338(17): 1174–1180. doi:10.1056/NEJM199804233381702
17. Di Girolamo N, Sarris M, Chui J, Cheema H, Coroneo MT, Wakefield D. Localization of the lowaffinity nerve growth factor receptor p75 in human limbal epithelial cells. J Cell Mol Med. 2008;12(6B): 2799–2811. doi:10.1111/j.1582-4934.2008.00290.x
18. Lambiase A, Bonini S, Manni L, Ghinelli E, Tirassa P, Rama P, Aloe L. Intraocular production and release of nerve growth factor after iridectomy. Invest Ophthalmol Vis Sci. 2002;43(7): 2334–2340.
19. Ghinelli E, Aloe L, Cortes M, Micera A, Lambiase A, Bonini S. Nerve growth factor (NGF) and lenses: effects of NGF in an in vitro rat model of cataract. Graefes Arch Clin Exp Ophthalmol. 2003;241(10): 845–851. doi:10.1007/s00417-003-0733-6
20. Muzi S, Colafrancesco V, Sornelli F, Mantelli F, Lambiase A, Aloe L. Nerve growth factor in the developing and adult lacrimal glands of rat with and without inherited retinitis pigmentosa. Cornea. 2010;29(10): 1163–1168. doi:10.1097/ICO.0b013e3181d3d3f9
21. Lambiase A, Bonini S, Micera A, Rama P, Bonini S, Aloe L. Expression of nerve growth factor receptors on the ocular surface in healthy subjects and during manifestation of inflammatory diseases. Invest Ophthalmol Vis Sci. 1998;39(7): 1272–1275.
22. Hah YS, Yoo WS, Seo S, Chung I, Kim HA, Cho HY, Kim SJ. Reduced NGF level promotes epithelial mesenchymal transition in human lens epithelial cells exposed to high dexamethasone concentrations. Curr Eye Res. 2019. doi:10.1080/02713683.2019.1695844
23. Lee HK, Lee KS, Kim HC, Lee SH, Kim EK. Nerve growth factor concentration and implications in photorefractive keratectomy vs laser in situ keratomileusis. Am J Ophthalmol. 2005;139(6): 965–971. doi:10.1016/j.ajo.2004.12.051
24. Micera A, Lambiase A, Aloe L, Bonini S, Levi- Schaffer F, Bonini S. Nerve growth factor involvement in the visual system: implications in allergic and neurodegenerative diseases. Cytokine Growth Factor Rev. 2004;15(6): 411–417. doi:10.1016/j.cytogfr.2004.09.003
25. Roberti G, Mantelli F, Macchi I, Massaro- Giordano M, Centofanti M. Nerve growth factor modulation of retinal ganglion cell physiology. J Cell Physiol. 2014;229: 1130–1133. doi:10.1002/jcp.24573
26. Amendola T, Fiore M, Aloe L. Postnatal changes in nerve growth factor and brain derived neurotrophic factor levels in the retina, visual cortex, and geniculate nucleus in rats with retinitis pigmentosa. Neurosci Lett. 2003;345(1): 37–40. doi:10.1016/s0304-3940(03)00491-9
27. Pizzorusso T, Fagiolini M, Gianfranceschi L, Porciatti V, Maffei L. Role of neurotrophins in the development and plasticity of the visual system: experiments on dark rearing. Int J Psychophysiol. 2000;35(2-3): 189–196. doi:10.1016/s0167-8760(99)00053-7
28. Rocco ML, Soligo M, Manni L, Aloe L. Nerve growth factor: early studies and recent clinical trials. Curr Neuropharmacol. 2018;16: 1455–1465. doi:10.2174/1570159X16666180412092859
29. Oddone F, Roberti G, Micera A, Busanello A, Bonini S, Quaranta L, Agnifili L, Manni G. Exploring serum levels of brain derived neurotrophic factor and nerve growth factor across glaucoma stages. PLoS One. 2017;12: e0168565. doi:10.1371/journal.pone.0168565
30. Park KS, Kim SS, Kim JC, Kim HC, Im YS, Anh CW, Lee HK. Serum and tear levels of nerve growth factor in diabetic retinopathy patients. Am J Ophthalmol. 2008;145(3): 432–437. doi:10.1016/j.ajo.2007.11.011
31. Xu JB, Yang XC, Guo JH, Chen JY, Shang Y, Liu WJ, Wang TM, Zou L. Serum nerve growth factor level indicates therapeutic efficacy of 125I seed implantation in advanced pancreatic adenocarcinoma. Eur Rev Med Pharmacol Sci. 2015;19(18): 3385–3390.
32. Carmignoto G, Maffei L, Candeo P, Canella R, Comelli C. Effect of NGF on the survival of rat retinal ganglion cells following optic nerve section. J Neurosci.1989;9(4): 1263–1272.
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