Офтальмохирургия. 2019; : 33-38
Исследование воздействия на сетчатку бинарной тампонады витреальной полости перфторорганическим соединением и силиконовым маслом в эксперименте in vivo
https://doi.org/10.25276/0235-4160-2019-1-33-38Аннотация
Цель. Изучение потенциального влияния бинарной тампонады витреальной полости (ВП) перфторорганическим соединением (ПФОС) и силиконовым маслом (СМ) на сетчатку в эксперименте in vivo в аспекте применения таковой бинарной тампонады в клинической практике, в частности, при отслойках сетчатки.
Материал и методы. Оценивалось воздействие на сетчатку комбинации перфтордекалина (ПФД) «Dk-line™» (Bausch&Lomb) и СМ «Oxane 1300™» (Bausch&Lomb) при их длительном нахождении в витреальной полости. В исследование вошли 90 кроликов (90 глаз) породы шиншилла. Кролики были поделены на 3 группы: кроликам I (опытной) группы в ВП правых глаз вводили комбинацию ПФД и СМ, кроликам II (контрольной) группы в правые глаза вводили СМ, кроликам III (контрольной) группы в правые глаза вводили ПФД. Сроки выведения из эксперимента составили 1, 2 и 3 мес. Проводились клиническое наблюдение и гистологическое исследование сетчатки.
Результаты. При нахождении исследуемой комбинации тампонирующих веществ в ВП на сроке наблюдения 1 мес. во всех группах сетчатка не отличалась от нормы, на сроке наблюдения 2 мес. во всех глазах наблюдался отёк сетчатки, на сроке наблюдения 3 мес. сетчатка во всех группах глаз была истончена. При этом по данным клинических наблюдений и исследования гистологической картины сетчатки не было выявлено различий между глазами, в ВП которых находилась комбинация тампонирующих веществ (ПФД+СМ), и глазами, в ВП которых находилось СМ.
Выводы. Не выявлено никаких признаков патологических изменений в сетчатке при длительности нахождения тампонирующих веществ 1 мес., что является признаком отсутствия какой бы то ни было опасности применения бинарной тампонады на этом сроке. При более длительном нахождении в ВП указанная комбинация веществ безопасна в той же мере, как и СМ.
Список литературы
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14. Mackiewicz J., Maaijwee K., Luke C., Kociok N., Hiebl W., Meinert H., Joussen A.M. Effect of gravity in long-term vitreous tamponade: in vivo investigation using perfluorocarbon liquids and semi-fluorinated alkanes. Graefes Arch. Clin. Exp. Ophthalmol. 2007;245: 665-675. Available from: https://doi.org/10.1007/ s00417-006-0414-3.
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19. Sparrow J.R., Matthews G.P., Iwamoto T., Ross R., Gershbein A., Chang S. Retinal tolerance to intravitreal perfluoroethylcyclohexane liquid in the rabbit. Retina. 1993;13(1): 56-62.
20. Stolba U., Krepler K., Velikay-Parel M., Binder S. The effect of specific gravity of perfluorocarbon liquid on the retina after experimental vitreous substitution. Graefes Arch. Clin. Exp. Ophthalmol. 2004;242(11): 931¬936. Available from: https://doi.org/10.1007/s00417- 004-0916-9.
21. Velikay M., Stolba U., Wedrich A., Li Y., Datlinger P., Binder S. The effect of the chemical stability and purification of perfluorocarbon liquids in experimental extended-term vitreous substitution. Graefes Arch. Clin. Exp. Ophthalmol. 1995;223: 26-30.
22. Velikay M., Wedrich A., Stolba U., Datlinger P, Li Y., Binder S. Experimental long-term vitreous replacement with purified and non purified perfluorodecaline. Am. J. Ophthalmol. 1993;116: 565-570.
23. Watt I.M. The Principles and Practice of Electron Microscopy Cambridge University Press, 1997.
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Fyodorov Journal of Ophthalmic Surgery. 2019; : 33-38
Effect of Double Endotamponade with Perfluorocarbon Liquid and Silicone Oil: Experimental Study in vivo
https://doi.org/10.25276/0235-4160-2019-1-33-38Abstract
Purpose. To examine how the retina is affected by double tamponade of the vitreous cavity with the combination of perfluorocarbon liquid (PFCL) and silicone oil (SO) in order to assess the prospect of such double tamponade employment in clinical practice, particularly in cases of retinal detachment.
Material and methods. We examined retinal response on combination of perfluorodecalin (PFD) «Dk-line» (Bausch & Lomb) and silicone oil «Oxane 1300» (Bausch & Lomb) during long term vitreous cavity tamponade. The study included 90 chinchilla rabbits (90 eyes). Rabbits were randomized into three groups: I (test group) - vitreous cavities of right eyes were filled with PFD+SO; II (control) - the same but with SO only; III (control) - the same but with PFD only. Rabbits of each group were euthanized after 1, 2, or 3 months. We performed clinical examination and morphological examination of the rabbit retinas.
Results. At 1 month, retina in all groups did not differ from the normal morphology. At 2 months in all groups we detected retinal edema. At 3 months in all groups retina has thinned. According to clinical findings and morphological study we found no difference between eyes filled with PFD+SO combination and eyes filled with SO.
Conclusion. No signs of pathological response were found in the retinal morphology after 1 month of PFD+SO combination stay in vitreous cavity, thus representing the absence of any risk of double tamponade during 1 month. During a longer-term tamponade of the vitreous cavity this combination is safe to the same extent as SO is.References
1. Gao Q.Y., Fu Y., Hui Y.N. Vitreous substitutes: challenges and directions. Int. J. Ophthalmol. 2015;8(3): 437-440. Available from: https://doi.org/10.3980/j. issn.2222-3959.2015.03.01.
2. Prazeres J., Magalhaes O.Jr., Lucatto L.F., Navarro R.M., Moraes N.S., Farah M.E., Maia A., Maia M. Heavy silicone oil as a long-term endotamponade agent for complicated retinal detachments. Biomed Res. Int. 2014:136031. Available from: https://doi. org/10.1155/2014/136031.
3. Russo A., Morescalchi F., Donati S., Gambicorti E., Azzolini C., Costagliola C., Semeraro F. Heavy and standard silicone oil: intraocular inflammation. Int Ophthalmol. 2018;38(2): 855-867. Available from: https://doi.org/10.1007/s10792-017-0489-3.
4. Lyskin P.V., Zakharov V.D., Kazimirova E.G. Perftororganicheskie soedineniya dlya tampona¬dy vitreal'noi polosti. Oftal'mokhirurgiya. 2014; 3: 86-92.
5. Lyskin P.V., Zakharov V.D., Shpak A.A., Ka-zimirova E.G., Pedanova E.K., Ogorodnikova S.N. Otsenka bezopasnosti binarnoi tamponady vit- real'noi polosti v khirurgicheskom lechenii ot-sloek setchatki. Oftal'mokhirurgiya. 2013;1: 22-26.
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7. Lyskin P.V., Kazimirova E.G., Perepukhov A.M. Fiziko-khimicheskie aspekty binarnoi tamponady (PFOS i SM) polosti steklovidnogo tela. Oftal'-mokhirurgiya. 2014;1: 64-67.
8. Lyskin P.V., Kazimirova E.G. Double Endotamponade with Perfluorodecalin and Silicone Oil in Retinal Detachment Surgery. Retina Today. May 2012: 45-50.
9. Joussen A.M., Kirchhof B., Schrage N., Ocklenburg C. Heavy silicone oil versus standard silicone oil as vitreous tamponade in inferior PVR (HSO Study): design issues and implications. Acta Ophthalmol. Scand. 2007;85(6): 623-630. Available from: https://doi. org/10.1111/j.1600-0420.2007.00898.x.
10. Rush R., Sheth S., Surka S., Ho I., Gregory-Roberts J. Postoperative perfluoro-n-octane tamponade for primary retinal detachment repair. Retina. 2012;32(6): 1114-1120. Available from: https://doi.org/10.1097/ IAE.0b013e31822f56f6.
11. Zenoni S., Comi N., Fontana P., Romano M.R. The combined use of heavy and light silicone oil in the treatment of complicated retinal detachment tamponade effect or filling effect? Ann. Acad. Med. Singapore. 2012;41(10): 440-443.
12. Flores-Aquilar M., Munguia D., Loeb E., Crapotta J.A., Vuong C., Shakiba S. Intraocular tolerance of perfluoro-octylbromide (Perflubrom). Retina. 1995;15: 3-13.
13. Liang C., Peyman G.A. Tolerance of extended- term vitreous replacement with perfluoro-n-octane and perfluoroperhydrophenanthrene mixture (phenoctane). Retina. 1999; 19(3): 2 30-237. Available from: https: //doi. org/10.1097/00006982-199905000-00009.
14. Mackiewicz J., Maaijwee K., Luke C., Kociok N., Hiebl W., Meinert H., Joussen A.M. Effect of gravity in long-term vitreous tamponade: in vivo investigation using perfluorocarbon liquids and semi-fluorinated alkanes. Graefes Arch. Clin. Exp. Ophthalmol. 2007;245: 665-675. Available from: https://doi.org/10.1007/ s00417-006-0414-3.
15. Zeana D., Becker J., Kuckelkorn R., Kirchhof B. Perfluorohexyloctane as a long-term vitreous tamponade in the experimental animal. Int. Ophthalmol. 1999; 23: 17-24.
16. Conway M.D., Peyman G.A., Karagorlu M. et al. Perfluoroctylbromide (PFOB) as a vitreous substitute in nonhuman primates. Int. Ophthalmol. 1993;17: 259-264. Available from: https://doi.org/10.1007/BF01007793.
17. Orzalesi N., Migliavacca L., Bottoni F., Miglior S. Experimental short-term tolerance to perfluorodecalin in the rabbit eye: a histopathological study. Curr. Eye Res. 1998;17: 828-835.
18. Souza E.V., Aihara T., Souza N.V., Coutinho Neto J. Sulfur hexafluoride gas, perfluorocarbon liquid, air and balanced salt solution retinal toxicity in rabbit eyes. Arq. Bras. Oftalmol. 2005;68(4): 511-515. Available from: https://doi.org/10.1590/S0004-27492005000400016.
19. Sparrow J.R., Matthews G.P., Iwamoto T., Ross R., Gershbein A., Chang S. Retinal tolerance to intravitreal perfluoroethylcyclohexane liquid in the rabbit. Retina. 1993;13(1): 56-62.
20. Stolba U., Krepler K., Velikay-Parel M., Binder S. The effect of specific gravity of perfluorocarbon liquid on the retina after experimental vitreous substitution. Graefes Arch. Clin. Exp. Ophthalmol. 2004;242(11): 931¬936. Available from: https://doi.org/10.1007/s00417- 004-0916-9.
21. Velikay M., Stolba U., Wedrich A., Li Y., Datlinger P., Binder S. The effect of the chemical stability and purification of perfluorocarbon liquids in experimental extended-term vitreous substitution. Graefes Arch. Clin. Exp. Ophthalmol. 1995;223: 26-30.
22. Velikay M., Wedrich A., Stolba U., Datlinger P, Li Y., Binder S. Experimental long-term vitreous replacement with purified and non purified perfluorodecaline. Am. J. Ophthalmol. 1993;116: 565-570.
23. Watt I.M. The Principles and Practice of Electron Microscopy Cambridge University Press, 1997.
24. Carvalho PJ.M. Medigao de propriedades termofisicas de perfluorocarbonetos e liquidos ionicos. Dissertagao de mestrado em engenharia quimica. Universidade de Aveiro, 2002. [Carvalho PJ.M. Measurement of thermophysical properties of perfluorocarbons and ionic liquids. Master’s thesis in chemical engineering, University of Aveiro, 2002 (in Portuguese).]
