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Журнал микробиологии, эпидемиологии и иммунобиологии. 2016; : 34-43

МОЛЕКУЛЯРНО-БИОЛОГИЧЕСКИЕ МАРКЕРЫ ГЕПАТИТА В У ПАЦИЕНТОВ С ФИБРОЗОМ/ЦИРРОЗОМ ПЕЧЕНИ В УЗБЕКИСТАНЕ

Останкова Ю. В., Семенов А. В., Файзуллаев Х. Н., Казакова Е. И., Козлов А. В., Мусабаев Э. И., Тотолян А. А.

https://doi.org/10.36233/0372-9311-2016-5-34-43

Аннотация

Цель. Оценить распространенность генетических вариантов вируса гепатита В у жителей из различных регионов Узбекистана, страдающих гепатитами разного генеза с различной степенью выраженности фиброза печени и циррозом. Материалы и методы. Материалом исследования служили плазма крови и биоптаты печени 39 пациентов с различной степенью выраженности фиброза печени и циррозом. Было применено генотипирование на основе прямого секвенирования Pre-Sl/Pre-S2/S области ДНК ВГВ. Результаты. Вирус гепатита В был выявлен в 32 образцах из представленных 39, частота встречаемости - 82% соответственно. На основании филогенетического анализа показано, что среди обследованных больных выявлен только генотип D, преобладал вирус гепатита В субтипа D1 (84,38%) по сравнению с субтипами D2 (3,12%) и D3 (12,5%). Заключение. Масштабное скринирование ВГВ в Центральной Азии позволит оценить пути распространения и время эволюционного разделения изолятов вируса. Понимание эпидемиологии инфекционного процесса важно для разработки программ по профилактике и лечению инфекции.
Список литературы

1. ДамиНовТА., Камилов А.И., Туйчиев Л.Н. идр. Клинико-эпидемиологические аспекты генотипов вируса гепатита В, встречающихся в Узбекистане. Вопросы современной педиатрии. 2003, 3 (2)6: 98-100.

2. Avazova D., Kurbanov F., Tanaka Y. et al. Hepatitis В virus transmission pattern and vaccination efficiency in Uzbekistan. J. Med. Virol. 2008, 80 (2): 217-224.

3. Branco F., Mattos A.A., Coral G.P. et al. Occult hepatitis В virus infection in patients with chronic liver disease due to hepatitis C virus and hepatocellular carcinoma in Brazil. Arq. Gastroenterol. 2007, 44 (1): 58-63.

4. Brichler S., Lagathu G., Chekaraou M.A. et al. African, Amerindian and European hepatitis В virus strains circulate on the Caribbean Island of Martinique. J. Gen. Virol. 2013, 94 (10): 2318-2329.

5. Guirgis B.S.S., Abbas R.O., Azzazy H.M.E. Hepatitis В virus genotyping: current methods and clinical implications. Int. J. Infect. Dis. 2010, 14: 941-953.

6. Kato H., Ruzibakiev R., Yuldasheva N. et al. Hepatitis В virus genotypes in Uzbekistan and validity of two different systems forgenotyping. J. Med. Virol. 2002, 67 (4): 477-483.

7. Khan A., Kurbanov E, Tanaka Y. et al. Epidemiological and clinical evaluation of hepatitis B, hepatitis C, and delta hepatitis viruses in Tajikistan. J. Med. Virol. 2008, 80 (2): 268-276.

8. Khedive A., Sanei-Moghaddam I., Alavian S.M. et al. Hepatitis В virus surface antigen (HBsAg) mutations are rare but clustered in immune epitopes in chronic carriers from Sistan-Balouchestan Province, Iran. Arch. Iran. Med. 2013, 16 (7): 385-389.

9. Kim K.H., Chang H.Y., Park J.Y. et al. Spontaneous HBsAg loss in Korean patients: relevance of viral genotypes, S gene mutations, and covalently closed circular DNA copy numbers. Clin. Mol. Hepatol. 2014, 20 (3): 251-260.

10. Kishk R., Atta H.A., Ragheb M. et al. Genotype characterization of occult hepatitis В virus strains among Egyptian chronic hepatitis C patients. East. Mediterr. Health. J. 2014,20 (2): 130-138. ll.Ozaras R., Inane B.I., Yemisen M. et al. Epidemiology of HBV subgenotypes D. Clin. Res. Hepatol. Gastroenterol. 2015, 39 (1): 28-37.

11. Pollicino T., Saitta C. Occult hepatitis В virus and hepatocellular carcinoma. World J. Gastroenterol. 2014, 20: 5951-5961.

12. Ramachandran S., Purdy M.A., Xia G. et al. Recent population expansions of hepatitis В virus in the United States. Virol. 2014, 88 (24): 13971-13980.

13. Rizvi M., Azam M., Sultan A. et al. Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India. Indian J. Pathol. Microbiol. 2014, 57 (4): 537-541.

14. Ruzibakiev R., Kato H., Ueda R. et al. Risk factors and seroprevalence of hepatitis В virus, hepatitis C virus, and human immunodeficiency virus infection in Uzbekistan. Intervirology. 2001,44 (6): 327-332.

15. Tallo T, Norder H., Tefanova V. et al. Hepatitis В virus genotype D strains from Estonia share sequence similarity with strains from Siberia and may specify ayw4. J. Med. Virol. 2004, 74: 221-227.

16. Tallo T, Tefanova V, Priimagi L. et al. D2: major subgenotype of hepatitis В virus in Russia and the Baltic region. J. Gen. Virol. 2008, 89: 1829-1839.

17. Yuen M.F., Lai C.L. Hepatitis В virus genotypes: natural history and implications for treatment. Expert. Rev. Gastroenterol. Hepatol. 2007, 1: 321-328.

18. Zehender G., Shkjezi R., Ebranati E. et al. Reconstruction of the epidemic history of hepatitis В virus genotype D in Albania. Infect. Genet. Evol. 2012, 12: 291-298.

Journal of microbiology, epidemiology and immunobiology. 2016; : 34-43

MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN

Ostankova Yu. V., Semenov A. V., Faizullaev Kh. N., Kazakova E. I., Kozlov A. V., Musabaev E. I., Totolyan A. A.

https://doi.org/10.36233/0372-9311-2016-5-34-43

Abstract

Aim. Evaluate prevalence of genetic variants of hepatitis В viruses in population of various regions of Uzbekistan with hepatitis of various genesis and different severity levels of liver fibrosis and cirrhosis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different severity levels of liver fibrosis and cirrhosis served as study material. Genotyping based on direct sequencing of Pre-Sl/Pre-S2/S HBV DNA region was applied. Results. Hepatitis В virus was detected in 32 samples ofthe 39 provided, frequency of occurrence - 82%, respectively. Phylogenetic analysis has shown, that only genotype D was detected among the examined patients, hepatitis В virus subtype D1 predominated (84.38%) compared with D2 (3.12%) and D3 (12.5%) subtypes. Conclusion. Large-scale sequencing of HBV in Central Asia will allow to evaluate routes of transmission and time of evolutionary separation of virus isolates. Understanding the epidemiology of the infectious process is important for development of programs for prophylaxis and therapy of the infection.
References

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2. Avazova D., Kurbanov F., Tanaka Y. et al. Hepatitis V virus transmission pattern and vaccination efficiency in Uzbekistan. J. Med. Virol. 2008, 80 (2): 217-224.

3. Branco F., Mattos A.A., Coral G.P. et al. Occult hepatitis V virus infection in patients with chronic liver disease due to hepatitis C virus and hepatocellular carcinoma in Brazil. Arq. Gastroenterol. 2007, 44 (1): 58-63.

4. Brichler S., Lagathu G., Chekaraou M.A. et al. African, Amerindian and European hepatitis V virus strains circulate on the Caribbean Island of Martinique. J. Gen. Virol. 2013, 94 (10): 2318-2329.

5. Guirgis B.S.S., Abbas R.O., Azzazy H.M.E. Hepatitis V virus genotyping: current methods and clinical implications. Int. J. Infect. Dis. 2010, 14: 941-953.

6. Kato H., Ruzibakiev R., Yuldasheva N. et al. Hepatitis V virus genotypes in Uzbekistan and validity of two different systems forgenotyping. J. Med. Virol. 2002, 67 (4): 477-483.

7. Khan A., Kurbanov E, Tanaka Y. et al. Epidemiological and clinical evaluation of hepatitis B, hepatitis C, and delta hepatitis viruses in Tajikistan. J. Med. Virol. 2008, 80 (2): 268-276.

8. Khedive A., Sanei-Moghaddam I., Alavian S.M. et al. Hepatitis V virus surface antigen (HBsAg) mutations are rare but clustered in immune epitopes in chronic carriers from Sistan-Balouchestan Province, Iran. Arch. Iran. Med. 2013, 16 (7): 385-389.

9. Kim K.H., Chang H.Y., Park J.Y. et al. Spontaneous HBsAg loss in Korean patients: relevance of viral genotypes, S gene mutations, and covalently closed circular DNA copy numbers. Clin. Mol. Hepatol. 2014, 20 (3): 251-260.

10. Kishk R., Atta H.A., Ragheb M. et al. Genotype characterization of occult hepatitis V virus strains among Egyptian chronic hepatitis C patients. East. Mediterr. Health. J. 2014,20 (2): 130-138. ll.Ozaras R., Inane B.I., Yemisen M. et al. Epidemiology of HBV subgenotypes D. Clin. Res. Hepatol. Gastroenterol. 2015, 39 (1): 28-37.

11. Pollicino T., Saitta C. Occult hepatitis V virus and hepatocellular carcinoma. World J. Gastroenterol. 2014, 20: 5951-5961.

12. Ramachandran S., Purdy M.A., Xia G. et al. Recent population expansions of hepatitis V virus in the United States. Virol. 2014, 88 (24): 13971-13980.

13. Rizvi M., Azam M., Sultan A. et al. Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India. Indian J. Pathol. Microbiol. 2014, 57 (4): 537-541.

14. Ruzibakiev R., Kato H., Ueda R. et al. Risk factors and seroprevalence of hepatitis V virus, hepatitis C virus, and human immunodeficiency virus infection in Uzbekistan. Intervirology. 2001,44 (6): 327-332.

15. Tallo T, Norder H., Tefanova V. et al. Hepatitis V virus genotype D strains from Estonia share sequence similarity with strains from Siberia and may specify ayw4. J. Med. Virol. 2004, 74: 221-227.

16. Tallo T, Tefanova V, Priimagi L. et al. D2: major subgenotype of hepatitis V virus in Russia and the Baltic region. J. Gen. Virol. 2008, 89: 1829-1839.

17. Yuen M.F., Lai C.L. Hepatitis V virus genotypes: natural history and implications for treatment. Expert. Rev. Gastroenterol. Hepatol. 2007, 1: 321-328.

18. Zehender G., Shkjezi R., Ebranati E. et al. Reconstruction of the epidemic history of hepatitis V virus genotype D in Albania. Infect. Genet. Evol. 2012, 12: 291-298.