Журнал микробиологии, эпидемиологии и иммунобиологии. 2019; : 56-64
Активность препаратов, содержащих эхинохром А, в отношении вируса простого герпеса 2-го типа in vitro и in vivo
https://doi.org/10.36233/0372-9311-2019-6-56-64Аннотация
Целью работы было изучение активности эхинохрома А – нафтохиноидного пигмента из морских ежей и его композиции с антиоксидантами в отношении вируса простого герпеса 2-го типа (ВПГ-2) in vitro и in vivo.
Материалы и методы. Штамм ВПГ-2 (G ATCC VR-734) выращивали на культуре клеток Vero. Цитотоксическую и анти-ВПГ-2 активность препаратов оценивали in vitro по жизнеспособности клеток и подавлению цитопатогенного действия вируса с помощью МТТ-анализа. Эффективность препаратов у мышей с генитальным герпесом оценивали, учитывая изменения их массы тела, средней продолжительности жизни и показателей вирусной нагрузки.
Результаты и обсуждение. Композиция антиоксидантов: эхинохрома А, аскорбиновой кислоты и α-токоферола (5 : 5 :1) – демонстрировала более высокую противовирусную активность, чем один эхинохром А. Пероральное введение мышам композиции антиоксидантов предотвращало гибель 90% инфицированных ВПГ-2 животных и достоверно снижало вагинальную вирусную нагрузку. Противовирусная активность эхинохрома А и композиции антиоксидантов, вероятно, обусловлена как вирусингибирующей активностью препаратов, так и их антиоксидантными свойствами.
Заключение. Результаты исследования позволяют рассматривать эхинохром А и композицию антиоксидантов на его основе как перспективные лекарственные средства, обладающие противовирусными свойствами.
Список литературы
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Journal of microbiology, epidemiology and immunobiology. 2019; : 56-64
Activity of compounds containing echinochrome A against herpes simplex virus type 2 in vitro and in vivo
https://doi.org/10.36233/0372-9311-2019-6-56-64Abstract
The aim of the work was to study the activity of echinochrome A, a naphthoquinoid pigment from sea urchins, and its antioxidant composition against herpes simplex virus type 2 (HSV-2) in vitro and in vivo.
Materials and methods. Strain HSV-2 (G ATCC VR-734) was grown in Vero cells. The cytotoxic and anti-HSV-2 activity of the compounds was assessed in vitro by the cell viability and by cytopathic effect inhibition of virus using MTT test. The efficacy of compounds in mice model of vaginitis caused by HSV-2 was determined by the average lifetime, body weight and viral load changes.
Results and discussion. The antioxidant composition (echinochrome A, ascorbic acid and α-tocopherol (5:5:1)), showed a higher antiviral efficacy than echinochrome A alone. Oral administration of the antioxidant composition protected 90% of the infected mice against death and reduced vaginal viral loads. The antiviral activity of echinochrome A and the antioxidant composition is probably due to the virus-inhibiting activity of the compounds and their antioxidant properties.
Conclusion. The results obtained allow considering the tested compounds as promising agents with antiviral properties.
References
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7. Stonik V.A., Gusev E.I., Martynov M.Yu., Guseva M.R., Shchukin I.A., Agafonova I.G., et al. New medications for treatment of hemorrhagic stroke. High-resolution MRI in evaluation of histochrome in experimental hemorrhagic stroke. Dokl. Boil. Sci. 2005; 405: 421-3.
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12. Prichard M.N., Kern E.R., Hartline C.B., Lanier E.R., Quenelle D.C. CMX001 Potentiates the Efficacy of Acyclovir in Herpes Simplex Virus Infections. Antimicrob. Agents Chemother. 2011; 55(10): 4728-34. Doi: https://doi.org/10.1128/AAC.00545-11
13. Reed L.J., Muench H. A simple method of estimating fifty percent’s endpoints. Am. J. Hyg. 1938; 27: 493-7.
14. Cymerys J., Chodkowski M., Słońska A., Krzyżowska M., Bańbura M.W. Disturbances of mitochondrial dynamics in cultured neurons infected with human herpesvirus type 1 and type 2. J. Neurovirol. 2019; June 3. Doi: https://doi.org/10.1007/s13365-019-00762-x
15. Santana S., Sastre I., Recuero M., Bullido M.J., Aldudo J. Oxidative stress enhances neurodegeneration markers induced by herpes simplex virus type1 infection in human neuroblastoma cells. PLoS One. 2013; 8(10): e75842. Doi: https://doi.org/0.1371/journal.pone.0075842
16. Fedoreyev S.A., Krylova N.V., Mishchenko N.P., Vasileva E.A., Pislyagin E.A., Iunikhina O.V., et al. Antiviral and antioxidant properties of echinochrome A. Mar. Drugs. 2018; 16(12). Doi: https://doi.org/10.3390/md16120509
17. Boominathan S.P., Sarangan G., Srikakulapu S., Rajesh S., Duraipandian C., Srikanth P. Antiviral activity of bioassay guided fractionation of Plumbago Zeylanica roots against Herpes simplex virus type 2. WJPPS. 2014; 3(12): 1003-17.
18. Roa-Linares V.C., Miranda-Brand Y., Tangarife-Castaño V., Ochoa R., García P.A., Castro M.Á., et al. Anti-Herpetic, Anti-Dengue and Antineoplastic Activities of Simple and Heterocycle-Fused Derivatives of Terpenyl-1,4-Naphthoquinone and 1,4-Anthraquinone. Molecules. 2019; 24(7). Doi: https://doi.org/10.3390/molecules24071279
