Журнал микробиологии, эпидемиологии и иммунобиологии. 2019; : 40-46
Энтеробактерии в кишечном микробиоценозе вич-инфицированных детей
https://doi.org/10.36233/0372-9311-2019-5-40-46Аннотация
Цель. Оценка видового состава, факторов вирулентности и характера взаимодействия энтеробактерий с кишечными микросимбионтами у детей с ВИЧ-инфекцией.
Материалы и методы. Изучен состав кишечного микробиоценоза 89 ВИЧ-инфицированных детей (основная группа) и 74 детей без ВИЧ-статуса (группа сравнения). Выделены и идентифицированы до вида 273 штамма энтеробактерий. Изучены частота и уровень экспрессии факторов адгезии, инвазии, токсинообразования и антагонизма.
Результаты. У ВИЧ-позитивных детей частота колонизации слизистой кишечника E.coli lac- была в 5 раз выше, E.coli hly+ в 1,5 раза выше, чем в группе сравнения. Количественный уровень условно патогенных энтеробактерий нарастает при усугублении микроэкологических нарушений; 98% энтеробактерий формирует многокомпонентные ассоциации. Из них 86,8% проявляет нейтрализм по отношению к другим симбионтам. Частота и уровень продукции факторов адгезии, инвазии и токсинообразования в сравниваемых группах не отличались.
Заключение. У ВИЧ-инфицированных детей формируется энтеробактериальный тип микробиоценоза. Биологические свойства энтеробактерий не зависят от наличия у детей ВИЧ-статуса.
Список литературы
1. Керимли Ф.И., Воробьева Ю.И., Козлов А.Е. и др. Микробиота кишечника при ВИЧ-инфекции. Бюллетень медицинских Интернет-конференций. 2017, 6 (7): 10-13.
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3. Boulougoura A., Sereti I. HIV infection and immune activation: the role of coinfections. Current Opinion in HIV and AIDS. 2016, 11(2): 191-200. doi: 10.1097/coh.0000000000000241.
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5. Dubourg G., Surenaud M., Lévy Y. et al. Microbiome of HIV-infected people. Microbial Pathogenesis. 2017, 106: 85-93.doi: 10.1016/j.micpath.2016.05.015.
6. Goedert J.J. Effects of HIV, immune deficiency, and confounding on the distal gut microbiota. EBioMedcine. 2016, 5: 14-15. doi: 10.1016/j.ebiom.2016.01.034.
7. Monaco C.L., Gootenberg D.B., Zhao G. et al. Altered virome and bacterial microbiome in Human immunodeficiency virus-associated acquired immunodeficiency syndrome. Cell Host and Microbe. 2016, 19(3): 311-322. doi: 10.1016/j.chom.2016.02.011.
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Journal of microbiology, epidemiology and immunobiology. 2019; : 40-46
Enterobacteria in the intestinal microbiocenosis of hivinfected children
https://doi.org/10.36233/0372-9311-2019-5-40-46Abstract
Aim. The purpose was to assess the species composition, virulence factors and the nature of the interaction of Enterobacteria with intestinal microsymbionts in HIV-positive children.
Material and methods. The composition of intestinal microbiocenosis of 89 HIV-infected children (main group) and 74 children without HIV status (comparison group) was studied. 273 strains of Enterobacteria were isolated and identified to the species. The frequency and level of expression of adhesion, invasion, toxin formation and antagonism factors were studied.
Results. In HIV-positive children colonization rate of intestinal mucosa E. coli lac — was 5 times higher, E. coli hly+ 1.5 times higher than in the comparison group. The quantitative level of opportunistic enterobacteria increases with the aggravation of microecological disorders, 98% of enterobacteria forms a many-to-many association. Of these, 86.8% are neutral towards other symbionts. The frequency and level of production of adhesion, invasion and toxin formation factors did not differ in the compared groups.
Conclusion. In HIV-infected children is formed enterobacteriales type microbiota. Biological properties of enterobacteria did not depend on the presence of HIV status in children.
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4. Burgener A., McGowan I., Klatt N.R. HIV and mucosal barrier interactions: consequences for transmission and pathogenesis. Current Opinion in Immunology. 2015, 36: 22-30. doi: 10.1016/j.coi.2015.06.004.
5. Dubourg G., Surenaud M., Lévy Y. et al. Microbiome of HIV-infected people. Microbial Pathogenesis. 2017, 106: 85-93.doi: 10.1016/j.micpath.2016.05.015.
6. Goedert J.J. Effects of HIV, immune deficiency, and confounding on the distal gut microbiota. EBioMedcine. 2016, 5: 14-15. doi: 10.1016/j.ebiom.2016.01.034.
7. Monaco C.L., Gootenberg D.B., Zhao G. et al. Altered virome and bacterial microbiome in Human immunodeficiency virus-associated acquired immunodeficiency syndrome. Cell Host and Microbe. 2016, 19(3): 311-322. doi: 10.1016/j.chom.2016.02.011.
8. Paquin-Proulx D., Ching C., Vujkovic-Cvijin I. et al. Bacteroides are associated with GALT iNK T cell function and reduction of microbial translocation in HIV-1 infection. Mucosal Immunology. 2016, 10(1): 69-78. doi: 10.1038/mi.2016.34.
9. Patel A.R., Shaha N.P., Prajapatia J.B. Immunomodulatory effects of probiotics in the treatment of human immunodeficiency virus (HIV) infection. Biomedicine and Preventive Nutrition. 2014, 4(1): 81-84. doi: 10.1016/j.bionut.2013.04.003.
10. Yilmas C., Gokmem V. Determination of tryptophan derivatives in kynurenine pathway in fermented foods using liquid chromatography tandem mass spectrometry. Food Chemistry. 2018, 243: 420-427. doi: 10.1016/j.foodchem.2017.10.004.
