Журнал микробиологии, эпидемиологии и иммунобиологии. 2019; : 110-117
Метод иммуно-ПЦР в диагностике бактериальных и вирусных инфекций
https://doi.org/10.36233/0372-9311-2019-3-110-117Аннотация
Иммуно-ПЦР (И-ПЦР) объединяет возможности двух современных диагностических методов: иммуноферментного анализа (ИФА) и полимеразной цепной реакции (ПЦР). Такое сочетание обусловливает 100-10000-кратное увеличение чувствительности по сравнению с аналогичным ИФА. В обзоре рассмотрены основные варианты И-ПЦР, приведены примеры возможного использования метода для ранней и ретроспективной диагностики различных заболеваний.
Список литературы
1. . Козырь А.В., Колесников А.В., Хлынцева А.Е.. Белова Е.В.. Шемякин И.Г. Способ определения наличия протективного антигена сибирской язвы на основе иммунодетекции, сопряженной с полимеразной цепной реакцией. Патент на изобретение № 2470307, 20.12.2012.
2. Козырь А.В., Колесников А.В. Хлынцева А.Е. Белова Е.В. Шемякин И.Г. Способ определения летального фактора сибирской язвы на основе иммунодетекции, сопряженной с полимеразной цепной реакцией. Патент на изобретение № 2486524, 27.06.2013.
3. Adams N.M., Jackson S.R., Haselton F.R. et al. Design, synthesis, and characterization of nucleic-acid functionalized gold surfaces for biomarker detection. J. Langmuir. 2012, 28:1068-1082.
4. Adler M., Schulz S., Fisher R., Niemeyer C.M. Detection of Rotavirus from stool samples using a standardized immuno-PCR («Imperacer») method with endpoint and real-time detection. Biochemical and Biophysical Research Communications. 2005, 333:1289-1294.
5. Adler M., Wacker R., Niemeyer C.M. Sensitivity by combination: immuno-PCR and related technologies. J. Analyst (London). 2008, 133:702-718.
6. Barletta J., Bartolome A., Constantine N.T Immunomagnetic quantitative immuno-PCR for detection of less than one HIV-1 virion. J. Virol. Methods. 2009, 157:122-132.
7. Chang TC., Huang S.H. A modified immuno-polymerase chain reaction for the detection of P-glucuronidase from Escherichia coli. J. Immunol. Methods. 1997, 208:35-42.
8. Chao H.Y., Wang YC., Tang S. et al. A highly sensitive immuno-polymerase chain reaction assay for Clostridium botulinum neurotoxin type A. J. Toxicon. 2004, 43:27-34.
9. Chen L., Wei H., Guo Y et al. Gold nanoparticle enhanced immuno-PCR for ultrasensitive detection of Hantaan virus nucleocapsid protein. J. Immunol. Methods. 2009. 346:64-70.
10. Chye S.M., Lin S.R., Chen YL. et al. Immuno-PCR for detection of antigen to Angiostrongylus cantonensis circulating fifth-stage worms. Clin. Chem. 2004. 50:51-57.
11. Cooper A., Williams N., Morris J. et al. ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis. J. Diagn. Microbiol. Infect. Dis. 2013, 75:135-138.
12. Deng M.J., Xiao X.Z., Zhang YM. et al. A highly sensitive immuno-PCR assay for detection of H5N1 avian influenza virus. Mol. Biol. Rep. 2011, 38:1941-1948.
13. Deng M., Long L., Xiao X. et al. Immuno-PCR for one step detection of H5N1 avian influenza virus and Newcastle disease virus using magnetic gold particles as carriers. Vet. Immunol. Immunopathol. 2011, 141:183-189.
14. Fischer A., von Eiff C., Kuczius T et al. A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins. J. Mol. Med. 2007, 85:461-469.
15. Guo Y C., Zhou Y F., Zhang X. E. et al. Phage display mediated immuno-PCR. Nucleic Acids Res. 2006, 34, e62.
16. He X., McMahon S., Skinner C. et al. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. J. Immunol. Methods. 2013, 389:18-28.
17. Huang S.H., Chang T.C. Detection of Staphylococcus aureus by a sensitive immuno-PCR assay. Clin. Chem. 2004, 50:1673-1674.
18. Kingston J., Saugata M., Uppalapati S.R. et al. Anthrax Outbreak Among Cattle and its Detection by Extractable Antigen 1 (EA1) Based Sandwich ELISA and Immuno PCR. Indian J. Microbiol. 2015, 55(1):29-34.
19. Kolesnikov A.V., Kozyr A.V., Ryabko A.K., Shemyakin I.G. Ultrasensitive detection of protease activity of anthrax and botulinum toxins by a new PCR-based assay. FEMS Pathog. Dis. 2016, 74:1-10.
20. Liang H., Cordova S.E., Kieft TL. et al. A highly sensitive immuno-PCR assay for detecting Group A Streptococcus. J. Immunol. Methods. 2003, 279:101-110.
21. Liu X., Xu Y., Xiong Y et al. VHH phage-based competitive real time immuno-polymerase chain reaction for ultrasensitive detection of ochra toxin A in cereal. Anal. Chem. 2014, 86:7471-7477.
22. Luk C., Compta Y., Magdalinou N. et al. Development and assessment of sensitive immuno-PCR assays for the quantification of cerebrospinal fluid three- and four-repeat tau isoforms in tauopathies. J. Neurochem. 2012, 123:396-405.
23. Maerle A.V., Ryazantsev D.Y, Dmitrenko O.A., Petrova E.E., Komaleva R.L., Sergeeva I.V., Trofimov D.Yu., Zavriev S.K. Detection of Staphylococcus aureus Toxins Using Immuno-PCR. Russian Journal of Bioorganic Chemistry. 2014, 40:526-531.
24. Maia M., Takahashi H., Adler K. et al. Development of a two-site immuno-PCR assay for hepatitis B surface antigen. J. Virol. Methods. 1995, 52:273-286.
25. Makam S., Majumder S., Kingston J. et al. Immuno capture PCR for rapid and sensitive identification of pathogenic Bacillus anthracis. World J. Microbiol. Biotechnol. 2013, 29(12):2379-2388.
26. Malou N., Raoult D. Immuno-PCR: a promising ultrasensitive diagnostic method to detect antigens and antibodies. Trends Microbiol. 2011, 19:295-302.
27. Malou N., Tran TN., Nappez C. et al. Immuno-PCR — a new tool for paleomicrobiology: the plague paradigm. PLoS ONE. 2012, 7, e31744.
28. Mehta P.K., Kalra M., Khuller G.K. et al. Development of an ultrasensitive polymerase chain reaction-amplified immunoassay based on mycobacterial RD antigens: implications for the serodiagnosis of tuberculosis. Diagn. Microbiol. Infect. Dis. 2012, 72:166-174.
29. Mehta P.K., Raj A., Singh N. et al. Diagnosis of extrapulmonary tuberculosis by PCR. FEMS Immunol. Med. Microbiol. 2012, 66: 20-36.
30. Monjezi R., Tan S.W., Tey B.T. et al. Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR. J. Virol. Methods. 2013, 187:121-126.
31. Morin I., Askin S.P., Schaeffer P.M. IgG-detection devices for the Tus-Ter-lock immuno-PCR diagnostic platform. J. Analyst. 2011, 136:4815-4821.
32. Niemeyer C.M., Adler M., Wacker R. Detecting antigens by quantitative immuno-PCR. Nat. Protoc. 2007, 2:1918-1930.
33. Niemeyer C.M., Adler M., Pignataro B. et al. Selfassembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR. Nucleic Acids Res. 1999, 27:4553-4561.
34. Perez J.W, Vargis E.A., Russ P.K. et al. Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction. Anal. Biochem. 2011, 410:141-148.
35. Rajkovic A., Moualij B., Uyttendaele M. et al. Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods. Appl. Environ. Microbiol. 2006, 72:6593-6599.
36. Ryazantsev D.Y., Petrova E.E., Kalinina N.A., Valyakina T.I., Grishin E.V., Zavriev S.K. Application of supramolecular DNA streptavidin complexes for ultrasensitive detection of several toxins by immuno-PCR. Global J. Analytical Chemistry. 2012, 3:e17.
37. Tian P., Mandrell R. Detection of norovirus capsid proteins in faecal and food samples by a real time immuno-PCR method. J. Appl. Microbiol. 2006, 100:564-574.
38. Wu H.C., Huang Y.L., Lai S.C. et al. Detection of Clostridium botulinum neurotoxin type A using immunoPCR. Lett. Appl. Microbiol. 2001, 32:321-325.
39. Zhang W, Bielaszewska M., Pulz M. et al. New immuno-PCR assay for detection of low concentrations of Shiga toxin 2 and its variants. J. Clin. Microbiol. 2006, 46:1292-1297.
Journal of microbiology, epidemiology and immunobiology. 2019; : 110-117
Method of Immuno-PCR in diagnostics of bacterial and viral infections
https://doi.org/10.36233/0372-9311-2019-3-110-117Abstract
Immuno-PCR (I-PCR) combines the capabilities of two modern diagnostic methods of enzyme immunoassay (ELISA) and polymerase chain reaction (PCR), combination these methods causes a 100-10000 fold increase in sensitivity compared to a similar ELISA. The review considers the main variants of I-PCR, gives examples of possible use of the method for early and retrospective diagnosis of various diseases.
References
1. . Kozyr' A.V., Kolesnikov A.V., Khlyntseva A.E.. Belova E.V.. Shemyakin I.G. Sposob opredeleniya nalichiya protektivnogo antigena sibirskoi yazvy na osnove immunodetektsii, sopryazhennoi s polimeraznoi tsepnoi reaktsiei. Patent na izobretenie № 2470307, 20.12.2012.
2. Kozyr' A.V., Kolesnikov A.V. Khlyntseva A.E. Belova E.V. Shemyakin I.G. Sposob opredeleniya letal'nogo faktora sibirskoi yazvy na osnove immunodetektsii, sopryazhennoi s polimeraznoi tsepnoi reaktsiei. Patent na izobretenie № 2486524, 27.06.2013.
3. Adams N.M., Jackson S.R., Haselton F.R. et al. Design, synthesis, and characterization of nucleic-acid functionalized gold surfaces for biomarker detection. J. Langmuir. 2012, 28:1068-1082.
4. Adler M., Schulz S., Fisher R., Niemeyer C.M. Detection of Rotavirus from stool samples using a standardized immuno-PCR («Imperacer») method with endpoint and real-time detection. Biochemical and Biophysical Research Communications. 2005, 333:1289-1294.
5. Adler M., Wacker R., Niemeyer C.M. Sensitivity by combination: immuno-PCR and related technologies. J. Analyst (London). 2008, 133:702-718.
6. Barletta J., Bartolome A., Constantine N.T Immunomagnetic quantitative immuno-PCR for detection of less than one HIV-1 virion. J. Virol. Methods. 2009, 157:122-132.
7. Chang TC., Huang S.H. A modified immuno-polymerase chain reaction for the detection of P-glucuronidase from Escherichia coli. J. Immunol. Methods. 1997, 208:35-42.
8. Chao H.Y., Wang YC., Tang S. et al. A highly sensitive immuno-polymerase chain reaction assay for Clostridium botulinum neurotoxin type A. J. Toxicon. 2004, 43:27-34.
9. Chen L., Wei H., Guo Y et al. Gold nanoparticle enhanced immuno-PCR for ultrasensitive detection of Hantaan virus nucleocapsid protein. J. Immunol. Methods. 2009. 346:64-70.
10. Chye S.M., Lin S.R., Chen YL. et al. Immuno-PCR for detection of antigen to Angiostrongylus cantonensis circulating fifth-stage worms. Clin. Chem. 2004. 50:51-57.
11. Cooper A., Williams N., Morris J. et al. ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis. J. Diagn. Microbiol. Infect. Dis. 2013, 75:135-138.
12. Deng M.J., Xiao X.Z., Zhang YM. et al. A highly sensitive immuno-PCR assay for detection of H5N1 avian influenza virus. Mol. Biol. Rep. 2011, 38:1941-1948.
13. Deng M., Long L., Xiao X. et al. Immuno-PCR for one step detection of H5N1 avian influenza virus and Newcastle disease virus using magnetic gold particles as carriers. Vet. Immunol. Immunopathol. 2011, 141:183-189.
14. Fischer A., von Eiff C., Kuczius T et al. A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins. J. Mol. Med. 2007, 85:461-469.
15. Guo Y C., Zhou Y F., Zhang X. E. et al. Phage display mediated immuno-PCR. Nucleic Acids Res. 2006, 34, e62.
16. He X., McMahon S., Skinner C. et al. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. J. Immunol. Methods. 2013, 389:18-28.
17. Huang S.H., Chang T.C. Detection of Staphylococcus aureus by a sensitive immuno-PCR assay. Clin. Chem. 2004, 50:1673-1674.
18. Kingston J., Saugata M., Uppalapati S.R. et al. Anthrax Outbreak Among Cattle and its Detection by Extractable Antigen 1 (EA1) Based Sandwich ELISA and Immuno PCR. Indian J. Microbiol. 2015, 55(1):29-34.
19. Kolesnikov A.V., Kozyr A.V., Ryabko A.K., Shemyakin I.G. Ultrasensitive detection of protease activity of anthrax and botulinum toxins by a new PCR-based assay. FEMS Pathog. Dis. 2016, 74:1-10.
20. Liang H., Cordova S.E., Kieft TL. et al. A highly sensitive immuno-PCR assay for detecting Group A Streptococcus. J. Immunol. Methods. 2003, 279:101-110.
21. Liu X., Xu Y., Xiong Y et al. VHH phage-based competitive real time immuno-polymerase chain reaction for ultrasensitive detection of ochra toxin A in cereal. Anal. Chem. 2014, 86:7471-7477.
22. Luk C., Compta Y., Magdalinou N. et al. Development and assessment of sensitive immuno-PCR assays for the quantification of cerebrospinal fluid three- and four-repeat tau isoforms in tauopathies. J. Neurochem. 2012, 123:396-405.
23. Maerle A.V., Ryazantsev D.Y, Dmitrenko O.A., Petrova E.E., Komaleva R.L., Sergeeva I.V., Trofimov D.Yu., Zavriev S.K. Detection of Staphylococcus aureus Toxins Using Immuno-PCR. Russian Journal of Bioorganic Chemistry. 2014, 40:526-531.
24. Maia M., Takahashi H., Adler K. et al. Development of a two-site immuno-PCR assay for hepatitis B surface antigen. J. Virol. Methods. 1995, 52:273-286.
25. Makam S., Majumder S., Kingston J. et al. Immuno capture PCR for rapid and sensitive identification of pathogenic Bacillus anthracis. World J. Microbiol. Biotechnol. 2013, 29(12):2379-2388.
26. Malou N., Raoult D. Immuno-PCR: a promising ultrasensitive diagnostic method to detect antigens and antibodies. Trends Microbiol. 2011, 19:295-302.
27. Malou N., Tran TN., Nappez C. et al. Immuno-PCR — a new tool for paleomicrobiology: the plague paradigm. PLoS ONE. 2012, 7, e31744.
28. Mehta P.K., Kalra M., Khuller G.K. et al. Development of an ultrasensitive polymerase chain reaction-amplified immunoassay based on mycobacterial RD antigens: implications for the serodiagnosis of tuberculosis. Diagn. Microbiol. Infect. Dis. 2012, 72:166-174.
29. Mehta P.K., Raj A., Singh N. et al. Diagnosis of extrapulmonary tuberculosis by PCR. FEMS Immunol. Med. Microbiol. 2012, 66: 20-36.
30. Monjezi R., Tan S.W., Tey B.T. et al. Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR. J. Virol. Methods. 2013, 187:121-126.
31. Morin I., Askin S.P., Schaeffer P.M. IgG-detection devices for the Tus-Ter-lock immuno-PCR diagnostic platform. J. Analyst. 2011, 136:4815-4821.
32. Niemeyer C.M., Adler M., Wacker R. Detecting antigens by quantitative immuno-PCR. Nat. Protoc. 2007, 2:1918-1930.
33. Niemeyer C.M., Adler M., Pignataro B. et al. Selfassembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR. Nucleic Acids Res. 1999, 27:4553-4561.
34. Perez J.W, Vargis E.A., Russ P.K. et al. Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction. Anal. Biochem. 2011, 410:141-148.
35. Rajkovic A., Moualij B., Uyttendaele M. et al. Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods. Appl. Environ. Microbiol. 2006, 72:6593-6599.
36. Ryazantsev D.Y., Petrova E.E., Kalinina N.A., Valyakina T.I., Grishin E.V., Zavriev S.K. Application of supramolecular DNA streptavidin complexes for ultrasensitive detection of several toxins by immuno-PCR. Global J. Analytical Chemistry. 2012, 3:e17.
37. Tian P., Mandrell R. Detection of norovirus capsid proteins in faecal and food samples by a real time immuno-PCR method. J. Appl. Microbiol. 2006, 100:564-574.
38. Wu H.C., Huang Y.L., Lai S.C. et al. Detection of Clostridium botulinum neurotoxin type A using immunoPCR. Lett. Appl. Microbiol. 2001, 32:321-325.
39. Zhang W, Bielaszewska M., Pulz M. et al. New immuno-PCR assay for detection of low concentrations of Shiga toxin 2 and its variants. J. Clin. Microbiol. 2006, 46:1292-1297.
