Журнал микробиологии, эпидемиологии и иммунобиологии. 2019; : 54-59
Применение молекулярно-генетического анализа и геномного профилирования возбудителей инфекционных болезней региона Сочи в период подготовки и проведения чемпионата мира по футболу FIFA-2018
https://doi.org/10.36233/0372-9311-2019-3-54-59Аннотация
При подготовке к проведению чемпионата мира по футболу FIFA-2018 в 2015-2017 гг. определены генетические особенности региональных штаммов-патогенов в г. Сочи. Типированы штаммы и изоляты нуклеиновых кислот возбудителей инфекционных болезней бактериальной и вирусной природы — ОКИ: рота-, норо-, астро и энтеровирусов, Salmonella enteritidis, природноочаговых инфекций: хантавирусов, риккетсий группы КПЛ, боррелий. В результате осуществлено комплексное генетическое профилирование актуальных для региона г. Сочи вариантов возбудителей инфекций. Полученная база данных использована для обеспечения молекулярного анализа штаммов-патогенов в период проведения XIX Международного фестиваля молодежи и студентов (2017 г.) и во время проведения чемпионата мира по футболу (2018 г.). Показана перспектива использования данного алгоритма генетического анализа при расшифровке вспышек (случаев) инфекционных болезней на конкретных территориях.
Список литературы
1. Куличенко А.Н., Волынкина А.С., Лисицкая Я.В., Котенев Е.С., Кузнецова И.В., Подколзин А.Т, Зайцева Е.В., Паркина Н.В., Оробей В.Г Генетическое профилирование актуальных для региона г.-к. Сочи возбудителей природно-очаговых и кишечных инфекций. Бактериология. 2016, 1(1):16-21.
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3. Fournier PE., Grunnenberger F., Jaulhac B. et al. Evidence of Rickettsia helvetica infection in humans, eastern France. Emerging Infectious Diseases. 2000, 6(4):389-392.
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6. Nilsson K., Elfving K., Pеhlson C. Rickettsia helvetica in Patient with Meningitis, Sweden, 2006. Emerging Infectious Diseases. 2010, 16(3): 490-492. doi:10.3201/eid1603.090184.
7. Pickett B.E., Sadat E.L., Zhang Y. et al. VIPR: an open bioinformatics database and analysis resource for virology research. Nucleic Acids Res. 2012, 40:D593-D598.
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9. Veselova О., Podkolzin А., Petukhov D. et al. Rotavirus Group A Surveillance and Genotype Distribution in Russian Federation in Seasons 2012-2013. International Journal of Clinical Medicine. 2014, 5:407413. doi: 10.4236/ijcm.2014.57055.
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Journal of microbiology, epidemiology and immunobiology. 2019; : 54-59
The application of molecular genetic analysis and genomic profiling of infectious diseases causative agents in Sochi during the preparation and holding of the Fifa World Cup 2018
https://doi.org/10.36233/0372-9311-2019-3-54-59Abstract
During the preparation of the FIFA World Cup 2018 in 2015-2017 genetic features of regional pathogenic strains in the city of Sochi are determined. The strains and nucleic acid isolates of bacterial and viral infectious diseases causative agents — acute intestinal infections: rota-, noro-, astro and enteroviruses, Salmonella enteritidis, natural focal infections: hantaviruses, Rickettsia of tick spotted fevers group, Borrelia are genotyped. In result, complex genetic profiling of infection diseases causative agents variants that are actual for the Sochi region has been carried out. The obtained database was used to provide molecular analysis of pathogens during the XIX International Festival of Youth and Students (2017) and during the FIFA World Cup (2018). The perspective of using this algorithm of genetic analysis in description of outbreaks (cases) of infectious diseases in specific areas is shown.
References
1. Kulichenko A.N., Volynkina A.S., Lisitskaya Ya.V., Kotenev E.S., Kuznetsova I.V., Podkolzin A.T, Zaitseva E.V., Parkina N.V., Orobei V.G Geneticheskoe profilirovanie aktual'nykh dlya regiona g.-k. Sochi vozbuditelei prirodno-ochagovykh i kishechnykh infektsii. Bakteriologiya. 2016, 1(1):16-21.
2. Dobec M., Golubic D., Punda-Polic V. et al. Rickettsia helvetic in Dermacentor reticulatus Ticks. Emerging Infectious Diseases. 2009,15(1):98-100. doi:10.3201/eid1501.080815.
3. Fournier PE., Grunnenberger F., Jaulhac B. et al. Evidence of Rickettsia helvetica infection in humans, eastern France. Emerging Infectious Diseases. 2000, 6(4):389-392.
4. Gonin P et al. Genetic Diversity and Molecular Epidemiology of Norwalk-like Viruses. The Journal of Infectious Diseases. 2000, 182:691—697.
5. Graaf M., Beek J., Vennema H. et al. Emergence of a novel GII.17 norovirus — End of the GII.4 era? Euro Surveill. 2015;20(26):pii=21178. Article DOI: http://dx.doi.org/10.2807/1560-7917.ES2015.20.26.21178.
6. Nilsson K., Elfving K., Pehlson C. Rickettsia helvetica in Patient with Meningitis, Sweden, 2006. Emerging Infectious Diseases. 2010, 16(3): 490-492. doi:10.3201/eid1603.090184.
7. Pickett B.E., Sadat E.L., Zhang Y. et al. VIPR: an open bioinformatics database and analysis resource for virology research. Nucleic Acids Res. 2012, 40:D593-D598.
8. Tkachenko E.A., Witkowski PT, Radosa L. et al. Adler Hantavirus, a New Genetic Variant of Tula Virus Identified in Major’s Pine Voles (MicrotusMajori) Sampled in Southern European Russia. Infection, Genetics and Evolution. Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases. 2015, 29:156-163. http://dx.doi.org/10.1016/j.meegid.2014.11.018.
9. Veselova O., Podkolzin A., Petukhov D. et al. Rotavirus Group A Surveillance and Genotype Distribution in Russian Federation in Seasons 2012-2013. International Journal of Clinical Medicine. 2014, 5:407413. doi: 10.4236/ijcm.2014.57055.
10. Vinje Jan. et al. Development and Application of a Capsid VP1 (Region D) Based Reverse Transcription PCR Assay for Genotyping of Genogroup I and II Noroviruses. Journal of Virological Methods. 2004, 116:109-117.
