НОВЫЙ ПОДХОД К ГЕНОТИПИРОВАНИЮ ГОСПИТАЛЬНЫХ ИЗОЛЯТОВ CLOSTRIDIUM DIFFICILE

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Журнал микробиологии, эпидемиологии и иммунобиологии. 2016; : 9-15

НОВЫЙ ПОДХОД К ГЕНОТИПИРОВАНИЮ ГОСПИТАЛЬНЫХ ИЗОЛЯТОВ CLOSTRIDIUM DIFFICILE

Терлецкий В. П., Тыщенко В. И., Новикова О. Б., Новикова И. И., Джавадов Э. Д.

https://doi.org/10.36233/0372-9311-2016-2-9-15

Аннотация

Цель. Разработка нового подхода в генотипировании Clostridium difficile и испытание его на примере 140 госпитальных изолятов. Материалы и методы. Подход основан на идее двойного расщепления и избирательного мечения (ДРИМ), использованного ранее при генотипировании других бактериальных патогенов. Был осуществлен подбор оптимальных ферментов рестрикции MluI и Mph1103I, оптимизированы условия проведения реакции ДРИМ. Результаты. Проведено генотипирование госпитальных изолятов C.difficile, рассчитан индекс дискриминации штаммов, сделаны выводы о возможностях метода в выяснении путей распространения и идентификации источников инфекции. Заключение. Разработанный метод генотипирования имеет ряд преимуществ над существующими методами и может применяться в решении вопросов эпидемиологии инфекций, вызванных C.difficile.

Список литературы

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Journal of microbiology, epidemiology and immunobiology. 2016; : 9-15

A NOVEL APPROACH TO GENOTYPING OF HOSPITAL ISOLATES OF CLOSTRIDIUM DIFFICILE

Terletsky V. P., Tyschenko V. I., Novikova O. B., Novikova I. I., Dzhavadov E. D.

https://doi.org/10.36233/0372-9311-2016-2-9-15

Abstract

Aim. Development of a novel approach in genotyping of Clostridium difficile and its testing on the example of 140 hospital isolates. Materials and methods. The approach is based on an idea of double digest and selective label (DDSL), used previously during genotyping of other bacterial pathogens. Selection of optimal enzymes for restriction of MluI and Mph1103I was carried out, condition of DDSL reaction execution were optimized. Results. Genotyping of C. difficile hospital isolates was carried out, index of strain discrimination was calculated, conclusions regarding possibilities of the method in elucidation of spread pathways and identification of infection sources were made. Conclusion. The developed method of genotyping has a number of advantages over the existing method and can be used to address issues in epidemiology of infections caused by C. difficile.

References

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2. Barlett J.C. Narrative review: the new epidemic of Clostridium difficile-associated enteric disease. Ann. Intern. Med. 2006, 145 (10): 758-764.

3. Bikandi J., San Millan R., Rementeria A. et al. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction. Bioinformatics. 2004, 20 (5): 798799.

5. Hunter P.R., Gaston M.A. Numerical index of the discriminatory ability of typing systems: an application of the Simpson’s index of diversity. J. Clin. Microbiol. 1988, 26: 2465-2466.

6. Joost I., Speck K., Herrmann M. et al. Characterization of Clostridium difficile isolates by slpA and tcdC gene sequencing. Int. J. Antimicrob. Agents. 2009, 33: 13-18.

12. Zaiss N.H., Rupnik M., Kuijper E.J. et al. Typing Clostridium difficile strains based on tandem repeat sequences. BMC Microbiol. 2009, 9: 6.

НОВЫЙ ПОДХОД К ГЕНОТИПИРОВАНИЮ ГОСПИТАЛЬНЫХ ИЗОЛЯТОВ CLOSTRIDIUM DIFFICILE

Аннотация

A NOVEL APPROACH TO GENOTYPING OF HOSPITAL ISOLATES OF CLOSTRIDIUM DIFFICILE

Abstract

События