ХАРАКТЕРИСТИКА КЛИНИЧЕСКИХ ИЗОЛЯТОВ ESCHERICHIA COLI, ВЫДЕЛЕННЫХ ОТ ПАЦИЕНТОВ С БОЛЕЗНЬЮ КРОНА

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Журнал микробиологии, эпидемиологии и иммунобиологии. 2017; : 42-49

ХАРАКТЕРИСТИКА КЛИНИЧЕСКИХ ИЗОЛЯТОВ ESCHERICHIA COLI, ВЫДЕЛЕННЫХ ОТ ПАЦИЕНТОВ С БОЛЕЗНЬЮ КРОНА

Городничев Р. Б., Ракитина Д. В., Манолов А. И., Байкова Ю. П., Щербаков П. Л., Смирнов Г. Б., Ильина Е. Н.

https://doi.org/10.36233/0372-9311-2017-6-42-49

Аннотация

Цель. Оценить пул E.coli внутри пораженной зоны кишечника пациентов с болезнью Крона. Материалы и методы. Клинические изоляты (28), а также контрольные образцы были протестированы относительно их возможной принадлежности к определенной филогенетической группе, способности к адгезии и инвазии на модели монослоя эпителиальных клеток СаСо2, способности к формированию биопленки и подвижности. Результаты. Было показано, что E.coli, высеянные из различного биоматериала, отличались по филогенетической принадлежности и способности образовывать биопленку. E.coli, относящиеся к типу адгезивно-инвазивных, обнаруживались преимущественно в материале биопсии слизистой кишечника пациентов с болезнью Крона. Заключение. Есть основания полагать, что тип адгезивно-инвазивных E.coli сложился на стыке экологических ниш просвета и стенки кишечника.

Список литературы

1. Ahmed I., Roy В. С., Khan S. A. et al. Microbiome, metabolome and inflammatory bowel disease. Microorganisms. 2016, 4 (2): 20.

2. Baumgart М., Dogan B., Rishniw M. et al. Culture independent analysis of ileal mucosa reveals a selective increase in invasive Escherichia coli of novel phylogeny relative to depletion of clostridiales in Crohn’s disease involving the ileum. ISME J. 2007, 1 (5): 403-418.

3. Carriere J., Darfeuille-Michaud A., Nguyen H. T. Infectious etiopathogenesis of Crohn's disease. World J. Gastroenterol. 2014, 20 (34): 12102-12117.

4. Comito D., Cascio A., Romano C. Microbiota biodiversity in inflammatory bowel disease. Italian J. Pediatrics. 2014, 40 (1): 1.

5. Clermont O., Bonacorsi S., Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Applied Environmental Microbiology. 2000, 66 (10): 4555-4558.

6. Liang X., Ji Y. Comparative analysis of staphylococcal adhesion and internalization by epithelial cells. Methicillin-resistant Staphylococcus aureus (MRSA) protocols. 2007, p. 145-151.

7. Martinez-Medina М., Garcia-Gil L.J. Escherichia coli in chronic inflammatory bowel diseases: An update on adherent invasive Escherichia coli pathogenicity. World J, Gastrointest. Pathophysiol. 2014, 5 (3): 213-227.

8. Martinez-Medina М., Aldeguer X., Lopez-Siles M. et al. Darfeuille-michaud a molecular diversity of Escherichia coli in the human gut: new ecological evidence supporting the role of adherent-invasive E. coli (AIEC) in Crohn's disease. Inflammatory Bowel Diseases. 2009, 15 (6): 872-882.

9. Wood Т.К., Barrios A.F.G., Herzberg М., Lee J. Motility influences biofilm architecture in Escherichia coli. Applied Microbiology Biotechnology. 2006, 72 (2): 361-367.

Journal of microbiology, epidemiology and immunobiology. 2017; : 42-49

FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE

Gorodnichev R. B., Rakitina D. V., Manolov A. I., Baykova Yu. P., Scherbakov P. L., Smirnov G. B., Ilina E. N.

https://doi.org/10.36233/0372-9311-2017-6-42-49

Abstract

Aim. To characterize pool of Crohn’s disease-associated E.coli isolated from patients with Crohn’s disease. Materials and methods. 28 clinical stains were selected. Clinical isolates, as well as control samples, were tested for their possible belonging to a certain phylogenetic group, the ability to adhere and invade on the model of a monolayer of CaCo2 epithelial cells, the ability to form biofilms and their mobility. Results. We have shown that E.coli, isolated from a different biomaterial, belonged to different phylogenetic groups and differed in their ability to form biofilms. Adhesive-invasive E.coli were found mainly in the material of biopsy of the intestinal mucosa of patients with Crohn’s disease. Conclusion. There are reasons to suppose that adhesive-invasive E.coli formed at the junction of ecological niches of the lumen and the intestinal wall.

References

1. Ahmed I., Roy V. S., Khan S. A. et al. Microbiome, metabolome and inflammatory bowel disease. Microorganisms. 2016, 4 (2): 20.

2. Baumgart M., Dogan B., Rishniw M. et al. Culture independent analysis of ileal mucosa reveals a selective increase in invasive Escherichia coli of novel phylogeny relative to depletion of clostridiales in Crohn’s disease involving the ileum. ISME J. 2007, 1 (5): 403-418.

3. Carriere J., Darfeuille-Michaud A., Nguyen H. T. Infectious etiopathogenesis of Crohn's disease. World J. Gastroenterol. 2014, 20 (34): 12102-12117.

4. Comito D., Cascio A., Romano C. Microbiota biodiversity in inflammatory bowel disease. Italian J. Pediatrics. 2014, 40 (1): 1.

5. Clermont O., Bonacorsi S., Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Applied Environmental Microbiology. 2000, 66 (10): 4555-4558.

6. Liang X., Ji Y. Comparative analysis of staphylococcal adhesion and internalization by epithelial cells. Methicillin-resistant Staphylococcus aureus (MRSA) protocols. 2007, p. 145-151.

7. Martinez-Medina M., Garcia-Gil L.J. Escherichia coli in chronic inflammatory bowel diseases: An update on adherent invasive Escherichia coli pathogenicity. World J, Gastrointest. Pathophysiol. 2014, 5 (3): 213-227.

8. Martinez-Medina M., Aldeguer X., Lopez-Siles M. et al. Darfeuille-michaud a molecular diversity of Escherichia coli in the human gut: new ecological evidence supporting the role of adherent-invasive E. coli (AIEC) in Crohn's disease. Inflammatory Bowel Diseases. 2009, 15 (6): 872-882.

9. Wood T.K., Barrios A.F.G., Herzberg M., Lee J. Motility influences biofilm architecture in Escherichia coli. Applied Microbiology Biotechnology. 2006, 72 (2): 361-367.

ХАРАКТЕРИСТИКА КЛИНИЧЕСКИХ ИЗОЛЯТОВ ESCHERICHIA COLI, ВЫДЕЛЕННЫХ ОТ ПАЦИЕНТОВ С БОЛЕЗНЬЮ КРОНА

Аннотация

FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE

Abstract

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