Журнал микробиологии, эпидемиологии и иммунобиологии. 2022; 99: 453-464
Первый случай выявления Listeria monocytogenes сиквенс-типов ST7, ST20, ST425 в сточных водах при обследовании водных объектов Вологодской области
https://doi.org/10.36233/0372-9311-266Аннотация
Введение. Listeria monocytogenes относится к числу значимых патогенов человека, вызывает различные формы листериоза, в том числе пищевые инфекции, менингиты, неонатальный сепсис, аборты. Листерии распространены во всех регионах мира.
Целью исследования явилось проведение микробиологического мониторинга Listeria monocytogenes в водных объектах вблизи животноводческих предприятий Вологодского района Вологодской области.
Материалы и методы. Выделение культур бактерий осуществляли титрационным и фильтрационным методами, идентифицировали с применением бактериологического, серологического методов, автоматизированных приборных методов, полногеномного секвенирования и биоинформационного анализа.
Результаты. Из 12 проанализированных образцов водных источников (6 образцов — сточные воды, 4 — речная вода, 2 — ливневые воды) выделены 3 штамма L. monocytogenes и один штамм L. innocua. Полногеномное секвенирование 3 штаммов L. monocytogenes установило их принадлежность к эволюционной линии II, к 3 сиквенс-типам и 2 серогруппам: ST425(1/2a-3a), ST20(1/2a-3a) и ST7(4a-4c). Показана принадлежность штаммов к категории множественно лекарственно-устойчивых, резистентных к 3 функциональным группам антимикробных препаратов (тетрациклинам, макролидам, сульфаниламидам). В геномах штаммов идентифицированы гены антибиотикорезистентности (fosX, pbp-like, lin, norB, sul), острова патогенности LIPI-1,LIPI-2, гены вирулентности inlABCJ, oatA, ami, gtcA, vip, lisK. У 1 штамма выявлен остров стрессоустойчивости SSI-1.
Заключение. Полученные данные свидетельствуют о контаминации водных источников вблизи животноводческих предприятий штаммами L. monocytogenes, обладающими высоким потенциалом патогенности, который может привести к вспышкам листериоза у людей, что указывает на необходимость тщательного мониторинга водных источников на наличие возбудителя листериоза и проведение профилактических и противоэпидемических мероприятий.
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Journal of microbiology, epidemiology and immunobiology. 2022; 99: 453-464
The first case of detection of Listeria monocytogenes sequence types ST7, ST20, ST425 in wastewater during an investigation of water bodies in the Vologda region
https://doi.org/10.36233/0372-9311-266Abstract
Introduction. Listeria monocytogenes is an important human pathogen causing various forms of listeriosis, including foodborne infections, meningitis, neonatal sepsis, and abortion. Listeria are common all over the world.
The purpose of the study was to conduct microbiological monitoring of L. monocytogenes in water reservoirs near livestock premises in the Vologda district of the Vologda region.
Materials and methods. Bacterial cultures were isolated using two methods, titration and filtration, followed by analysis using methods of conventional bacteriology, serotyping, and species identification by instrumental procedures such as whole genome sequencing, and bioinformatic analysis.
Results. Three isolates of L. monocytogenes and one isolate of Listeria innocua were isolated from 12 analyzed water samples (wastewater — 6, river water — 4, and storm water — 2 samples). whole genome sequencing of three L. monocytogenes strains attributed them to the evolutionary line II, and to three sequence types and two serogroups ST425(1/2a-3a), ST20(1/2a-3a), ST7 (4a-4c). The strains are shown to belong to multiple drug resistant ones conferring resistance to three functional groups of antibacterials such as tetracyclines, macrolides, and sulfonamides. Antibiotic resistance genes (fox, psp-like, lin,norB,sul), virulence Islands LIPI-1 and LIPI-2, and virulence genes inlABCJ, oatA, ami, gtcA, vip, and lisK in genomes of the strain were identified. Stress tolerance Island SSI-1 was identified in one strain.
Conclusions. The data obtained indicate contamination of water sources near the livestock premises with L. monocytogenes strains possessing high pathogenic potentiality for outbreaks of listeriosis in humans. This shows the necessity of careful monitoring of water sources for the presence of the causative agent of listeriosis as well as the implementing of anti-epidemic measures.
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27. Maung A.T., Mohammadi T.N., Nakashima S., Liu P., MasudaY., Honjoh K.I., et al. Antimicrobial resistance profiles of Listeria monocytogenes isolated from chicken meat in Fukuoka, Japan. Int. J. Food Microbiol. 2019; 304: 49–57. https://doi.org/10.1016/j.ijfoodmicro.2019.05.016
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