Журнал микробиологии, эпидемиологии и иммунобиологии. 2021; 98: 397-415
Метаболомное и экспосомное профилирование клинических образцов от пациентов с COVID-19 в Индии
https://doi.org/10.36233/0372-9311-161Аннотация
Введение. COVID-19 стал глобальной проблемой начиная с января 2020 г. В Индии локдаун был введен 22 марта 2020 г. вследствие резкого роста числа пациентов с COVID-19 в крупных городах и штатах страны. Данное исследование посвящено изучению роли метаболитов в прогнозе исхода инфекции, вызываемой SARS-CoV-2.
Материалы и методы. Выполнено метаболомное профилирование 106 образцов плазмы и 24 образцов мазков от индийских пациентов с клиническими проявлениями инфекции, проживавших в регионе Мумбаи. Образцы плазмы и мазков пациентов с положительным результатом на COVID-19 были дополнительно разделены на две группы в соответствии с нетяжёлым и тяжёлым течением COVID-19.
Результаты. В результате анализа первичных данных были обнаружены 7949 и 12 871 метаболитов в образцах плазмы и мазков соответственно. По сравнению с COVID-19-отрицательными образцами в образцах плазмы и мазков от пациентов с COVID-19 были обнаружены 11 и 35 значительно изменённых метаболитов соответственно. Кроме того, в образцах плазмы и мазков от пациентов с тяжёлым COVID-19 выявлены 9 и 23 метаболита соответственно, значительно изменённые по сравнению с образцами от пациентов с нетяжёлым течением COVID-19. Обнаружено, что COVID-19 оказывает наибольшее влияние на метаболические пути, связанные с метаболизмом аминокислот, сфингозина и солей желчных кислот.
Заключение. Результаты данного исследования способствуют идентификации потенциальных кандидатов в биомаркёры на основе метаболитов для быстрой диагностики и прогноза в клинической практике.
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Journal of microbiology, epidemiology and immunobiology. 2021; 98: 397-415
Metabolome and exposome profiling of the biospecimens from COVID-19 patients in India
https://doi.org/10.36233/0372-9311-161Abstract
Introduction. COVID-19 has become a global impediment by bringing everything to a halt starting from January 2020. India underwent the lockdown starting from 22nd March 2020 with the sudden spike in the number of COVID-19 patients in major cities and states. This study focused on how metabolites play a crucial role in SARSCoV-2 prognosis.
Materials and methods. Metabolome profiling of 106 plasma samples and 24 swab samples from symptomatic patients in the Indian population of the Mumbai region was done. COVID-19 positive samples were further segregated under the non-severe COVID-19 and severe COVID-19 patient cohort for both plasma and swab.
Results. After analyzing the raw files, total 7,949 and 12,871 metabolites in plasma and swab were found. 11 and 35 significantly altered metabolites were found in COVID-19 positive compared to COVID-19 negative plasma and swab samples, respectively. Also, 9 and 23 significantly altered metabolites were found in severe COVID-19 positive to non-severe COVID-19 positive plasma and swab samples, respectively. The majorly affected pathways in COVID-19 patients were found to be the amino acid metabolism pathway, sphingosine metabolism pathway, and bile salt metabolism pathway.
Conclusion. This study facilitates identification of potential metabolite-based biomarker candidates for rapid diagnosis and prognosis for clinical applications.
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