Морской гидрофизический журнал. 2019; 35: 481-495
Концентрация и флуоресценция хлорофилла а в период сезонной стратификации вод в Черном море
https://doi.org/10.22449/0233-7584-2019-5-481-495Аннотация
Цель. В настоящее время широко распространен экспрессный метод оценки концентрации хло-рофилла а по его флуоресценции. Применяемый при расчетах коэффициент связи между этими двумя величинами предполагается постоянный (в пространстве и во времени), что не соответствует действительности. Поэтому основной целью работы было исследовать зависимость флуоресценции от концентрации хлорофилла а в глубоководной части Черного моря в теплый период года, когда наблюдается сезонная стратификация вод.
Методы и результаты. Была исследована связь между коэффициентом поглощения света пигментами фитопланктона, концентрацией и флуоресценцией хлорофилла а. Показано, что соотношение между флуоресценцией и концентрацией хлорофилла а различалось между квазиизолированными слоями в период сезонной стратификации вод. Установлена вертикальная неоднородность флуоресценции, нормированной на концентрацию хлорофилла а в эвфотическом слое, обусловленная изменением способности фитопланктона поглощать кванты света на длине волны ~ 455 нм (возбуждение флуоресценции) в расчете на единицу содержания хлорофилла а. Сравнение удельных величин флуоресценции и показателя поглощения света на длине волны ~ 455 нм показало сильную корреляционную зависимость между ними, что свидетельствует о незначительном изменении величин квантового выхода флуоресценции в пределах зоны фотосинтеза.
Выводы. Полученные зависимости между концентрацией хлорофилла а и его флуоресценцией могут быть применены для уточнения используемых в настоящее время алгоритмов восстановления профилей концентрации хлорофилла а на основе данных погружных датчиков флуоресценции или самостоятельно дрейфующих буев международного проекта Biogeochemical-Argo.
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Morskoy Gidrofizicheskiy Zhurnal. 2019; 35: 481-495
Fluorescence of Chlorophyll a During Seasonal Water Stratification in the Black Sea
https://doi.org/10.22449/0233-7584-2019-5-481-495Abstract
Purpose. At present the express method of estimating the chlorophyll a concentration by its fluorescence is widely spread. The applied in calculations coupling coefficient between these two values is assumed to be constant (in time and space); but it does not correspond to reality. Therefore, the basic purpose of the paper consists in studying dependence of fluorescence on the chlorophyll a concentration in the deep part of the Black Sea in a warm period when seasonal water stratification is observed.
Methods and Results. Relation between the light absorption by the phytoplankton pigments, and the chlorophyll a concentration and fluorescence is investigated. It is shown that the ratio between the chlorophyll a concentration and fluorescence was different in the quasi-isolated layers during the seasonal water stratification. Revealed is the vertical heterogeneity of fluorescence normalized for the chlorophyll a concentration in the euphotic layer. It is conditioned by change of the phytoplankton ability to absorb light quantums the wavelength ~ 455 nm (fluorescence excitation) calculated for a unit of the chlorophyll a content. Comparison of the specific values of fluorescence and light absorption index on the wavelength ~ 455 nm showed their strong mutual correlation that testifies to insignificant variation of the values of the fluorescence quantum yield within the photosynthesis zone.
Conclusions. The obtained dependences between the chlorophyll a concentration and fluorescence can be used to specify the algorithms applied at present for reconstructing the chlorophyll a concentration profiles based on the data of either the fluorescence submerged probes or the drifting floats of the international project Biogeochemical Argo.References
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3. Combined processing and mutual interpretation of radiometry and fluorimetry from autonomous profiling Bio‐Argo floats: Chlorophyll a retrieval / X. Xing [et al.] // Journal of Geophysical Research: Oceans. 2011. Vol. 116, iss. C6. C06020. https://doi.org/10.1029/2010JC006899
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