Вопросы гематологии/онкологии и иммунопатологии в педиатрии. 2018; 17: 104-107
Морфологическое исследование костного мозга при врожденной дизэритропоэтической анемии: селективное обогащение популяций клеток для световой и электронной микроскопии с помощью биочипа и центрифугирования в градиенте плотности
https://doi.org/10.24287/1726-1708-2018-17-1-104-107Аннотация
Список литературы
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Pediatric Hematology/Oncology and Immunopathology. 2018; 17: 104-107
Bone marrow cell morphology in congenital diserythropoietic anemia: selective enrichment of the studied cell population for light and electron microscopy using a microarray and centrifugation in a density gradient
https://doi.org/10.24287/1726-1708-2018-17-1-104-107Abstract
Congenital diserythropoietic anemias are a group of rare inherited diseases causing uneffective erythropoiesis and multiple morphological changes in erythroblasts. Their differential diagnosis involves a study of the morphology of minor bone marrow cell populations that requires a selective cell enrichment. Here we describe two methods of the population enrichment by erythroid cells (including the self-developed cell-binding microarray) for their subsequent study by methods of light and electron microscopy.
References
1. Heimpel H., Kellermann K., Neuschwander N., Högel J., Schwarz K. The morphological diagnosis of congenital dyserythropoietic anemia: results of a quantitative analysis of peripheral blood and bone marrow cells. Haematologica 2010; 95 (6): 1034-6.
2. Bain B.J. The bone marrow aspirate of healthy subjects. Br J Hematol 1996; 94: 206-9.
3. Gambale A., Iolascon A., Andolfo I., Russo R. Diagnosis and management of congenital dyserythropoietic anemias. Expert review of hematology 2016; 9 (3): 283-96.
4. Heimpel H., Forteza-Vila J., Queisser W., Spiertz E. Electron and light microscopic study of the erythroblasts of patients with congenital dyserythropoietic anemia. Blood 1971; 37 (3): 299-310.
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