Андрология и генитальная хирургия. 2024; 25: 52-58
Влияние послеоперационного гипотиреоза на гормональную регуляцию сперматогенеза и функциональное состояние семенников у экспериментальных животных
https://doi.org/10.62968/2070-9781-2024-25-3-52-58Аннотация
Цель исследования: определить влияние послеоперационного гипотиреоза на сперматогенез у экспериментальных собак.
Материалы и методы: экспериментальные исследования были выполнены на 20 беспородных собаках-кобелях. Исследование было рандомизированным, выделены три группы. Первая группа – контрольная, контролем служили четыре собаки. Во второй группе была проведена гемитиреоидэктомия, третьей группе – субтотальная тиреоидэктомия. Операции проводились под общим обезболиванием. Объектом исследования служили семенники, щитовидная железа и кровь. В послеоперационном периоде был проведен биохимический и гистологический анализы удаляемой ткани щитовидной железы и семенников обследуемых собак. Определение гормонов в крови до и после операции проводилось с помощью хемилюминесцентного анализатора Roche Codas E601 (Швейцария).
Результаты: в ходе проведенного исследования в послеоперационном периоде у экспериментальных собак в сыворотке крови на 5–8-е сутки имело место уменьшение тестостерона на 15%, а на 20-е – с 15 до 30,1%; у собак после субтотальной тиреоидэктомии этот показатель на 20-е сутки составил 41,1% (р < 0,005). Изменение ФСГ и пролактина в крови было различно во всех группах. Показатель ФСГ на 5–8-е сутки имел достоверное повышение, а на 20-е – снижение, а показатель пролактина наоборот: уменьшение на 5–8 сутки и повышение на 20-е сутки. Гормональная активность щитовидной железы была снижена, особенно после субтотальной тиреоидэктомии. Морфологические исследования показали заметное снижение активности сперматогенного слоя и интерстициальный отек семенников на 5–8-е сутки в обеих группах. На 30-е сутки в обеих группах после операции отмечается заметное снижение как сперматогенного слоя, так и интерстициальных клеток семенников.
Заключение: результаты экспериментальных исследований показали, что в результате гемитиреоидэктомии и субтотальной тиреоидэктомии отмечаются заметное изменение регуляции сперматогенеза и структурно-функциональные изменения семенников экспериментальных животных, особенно в йододефицитом регионе.
Список литературы
1. Каркищенко В.Н, Семенов Х.Х., Каркишенко Н.Н. Влияние острой гипербарической гипоксии на репродуктивную функцию лабораторных крыс и мышей. Биомедицина 2015; 1: 60-65.
2. Ariyaratne H.B., Mills N., Mason I.J., Mendis-Handagama S.M. Effects of thyroid hormone on Leydig cell regeneration in the adult rat following ethane dimethane sulphonate treatment. Biol. Reprod. 2000; 63: 1115-23.
3. Bassett J.H., Harvey C.B. & Williams. Mechanisms of thyroid hormone receptor-specific nuclear and extra nuclear actions. Molecular and Cellular Endocrinology. 2003; 213:1-11.
4. Budi A., Legge F.S., Hentlein H, Yarovsky I. Electric field effects on insulin chain-B conformation. J. Phys. Chem. B. 2005; 109(7): 22641-22648.
5. Carle A., Pedersen I.B., Knudsen N., Perrild H., Ovesen L., Andersen S., et al. Hypothyroid symptoms fail to predict thyroid insufficiency in old people: a Population-Based Case-Control Study. Am J Меd. 2016; 129:1082- 92.
6. Cerillo A.G., Storti S., Kallushi E., Haxhiademi D., Miceli A., Murzi M., et al. The low triiodothyronine syndrome: a strong predictor of low cardiac output and death in patients undergoing coronary artery bypass grafting. Ann. Thorac. Surg. 2014: 97: 2089-95.
7. Gilleron J., Nebout М., Scarabelli L., Senegas-Balas F., Palmero S., Segretain D., Pointis G. A potential novel mechanism involving connexin 43 gap junction for control of Sertoli cells cell proliferation by thyroid hormones. Journal of Cellular Physiology. 2006; 209:153-161.
8. Hamouli-Said Z., Tahari F., Hamoudi F., Hadj-Bekkouche F. Comparative study of the effects of pre and post natal administration of a thyroid drug on testicular activity in adult rat. Folia Histochem Cytobiol. 2007; 45 Suppl.l 1:51- 57.
9. Hermandez A. Thyroid hormone Role and economy in the Developing testis. Vitamins and Hormones, 2018, 106: 473-500.
10. Jannini E.A., Olivieri М., Francavilla S., Gulino A., Ziparo E., Darmiento M. Ontogenesis patteilull^yroidliormone receptor in the rat testis. Endocrinology. 1990;126:2521-2526.
11. Kotwal SK, Kotwal S, Gupta R Singh JB, Mahajan A. Cerebellar ataxia as presenting feature of hypothyroidism. Arch Endocrinol Metab. (2016) 60:183-5.
12. Lombardo F., SgdrolT, Salacone P., Gilio B., Gandini L., Dondero F., Jannini E., Lenzi A. An-drogens and fertility. J. Endocrinol. Invest. 2005; 28(3):51-55.
13. Mendis-Handagama S. Effects of thyroid hormones on Leydid cells in the postnatal testis //Histology and Histopathology. 2004. 19:985-997.
14. Odermatt A., Strajhar P., Engeli R.T. Disruption of steroidogenesis: cell models for mechanistic investigations and as screening tools. J. Steroid Biochem. Mol. Biol. 2016;158:9-21.
15. Ono Y., Ono S., Yasunaga H., Matsui H., Fushimi K., Tanaka Y. Clinical characteristics and outcomes of myxedema coma: analysis of a national inpatient database in Japan. J Epidemiol. (2017) 27:117-22.
16. Ozgtiner М., Senol A., Ural М., Ifler M. The histological changes caused by experimental hypothyroid in the testicular tissue of adult rats. Suleyman Demirel University. J Eacult. Med. 2004;11:1- 6.
17. Palace M.R. Perioperative management of thyroid dysfunction. Health Servl Insights. (2017) 10:1178632916689677.
18. Roger Т., Cornelia F. Currently available murine leydigcell lines can be applied to study early steps of steroidogenesis bat not testosterone synthesis. Hellion, 2018.00527.
Andrology and Genital Surgery. 2024; 25: 52-58
Regulation of spermatogenesis and functional state of seeds at experimental animals
https://doi.org/10.62968/2070-9781-2024-25-3-52-58Abstract
Materials and Methods: experimental studies were performed on 20 mongrel male dogs. The study was randomized, 3 groups were identified. The first group was a control group, 4 dogs served as controls. Hemithyroidectomy was performed in the second group, subtotal thyroidectomy was performed in the third group. The operations were performed under general anesthesia. The testes, thyroid gland and blood were the objects of the study. In the postoperative period, biochemical and histological analyses of the removed thyroid gland tissue and testicles of the examined dogs were performed. The determination of hormones in the blood before and after surgery was carried out using a chemiluminescent analyzer Roche Codas E601 (Switzerland).
Results: in the course of the study, in the postoperative period, experimental dogs had a decrease in testosterone in serum by 15% on day 5-8, and from 15% to 30.1% on day 20; in dogs after subtotal thyroidectomy, this indicator was 41.1% on day 20 (p < 0.005). Changes in FSH and prolactin in the blood were different in all groups. The FSH index had a significant increase on day 5-8, and a decrease on day 20, and the prolactin index on the contrary: a decrease on day 5-8 and an increase on day 20. The hormonal activity of the thyroid gland was reduced, especially after subtotal thyroidectomy. Morphological studies have shown a marked decrease in the activity of the spermatogenic layer and interstitial edema of the testes on 5-8 days in both groups. On the 30th day, in both groups after surgery, there was a noticeable decrease in both the spermatogenic layer and the interstitial cells of the testes.
Conclusion: the results of experimental studies have shown that as a result of hemithyroidectomy and subtotal thyroidectomy, there is a noticeable change in the regulation of spermatogenesis and structural and functional changes in the testes of experimental animals, especially in the iodine-deficient region.
References
1. Karkishchenko V.N, Semenov Kh.Kh., Karkishenko N.N. Vliyanie ostroi giperbaricheskoi gipoksii na reproduktivnuyu funktsiyu laboratornykh krys i myshei. Biomeditsina 2015; 1: 60-65.
2. Ariyaratne H.B., Mills N., Mason I.J., Mendis-Handagama S.M. Effects of thyroid hormone on Leydig cell regeneration in the adult rat following ethane dimethane sulphonate treatment. Biol. Reprod. 2000; 63: 1115-23.
3. Bassett J.H., Harvey C.B. & Williams. Mechanisms of thyroid hormone receptor-specific nuclear and extra nuclear actions. Molecular and Cellular Endocrinology. 2003; 213:1-11.
4. Budi A., Legge F.S., Hentlein H, Yarovsky I. Electric field effects on insulin chain-B conformation. J. Phys. Chem. B. 2005; 109(7): 22641-22648.
5. Carle A., Pedersen I.B., Knudsen N., Perrild H., Ovesen L., Andersen S., et al. Hypothyroid symptoms fail to predict thyroid insufficiency in old people: a Population-Based Case-Control Study. Am J Med. 2016; 129:1082- 92.
6. Cerillo A.G., Storti S., Kallushi E., Haxhiademi D., Miceli A., Murzi M., et al. The low triiodothyronine syndrome: a strong predictor of low cardiac output and death in patients undergoing coronary artery bypass grafting. Ann. Thorac. Surg. 2014: 97: 2089-95.
7. Gilleron J., Nebout M., Scarabelli L., Senegas-Balas F., Palmero S., Segretain D., Pointis G. A potential novel mechanism involving connexin 43 gap junction for control of Sertoli cells cell proliferation by thyroid hormones. Journal of Cellular Physiology. 2006; 209:153-161.
8. Hamouli-Said Z., Tahari F., Hamoudi F., Hadj-Bekkouche F. Comparative study of the effects of pre and post natal administration of a thyroid drug on testicular activity in adult rat. Folia Histochem Cytobiol. 2007; 45 Suppl.l 1:51- 57.
9. Hermandez A. Thyroid hormone Role and economy in the Developing testis. Vitamins and Hormones, 2018, 106: 473-500.
10. Jannini E.A., Olivieri M., Francavilla S., Gulino A., Ziparo E., Darmiento M. Ontogenesis patteilull^yroidliormone receptor in the rat testis. Endocrinology. 1990;126:2521-2526.
11. Kotwal SK, Kotwal S, Gupta R Singh JB, Mahajan A. Cerebellar ataxia as presenting feature of hypothyroidism. Arch Endocrinol Metab. (2016) 60:183-5.
12. Lombardo F., SgdrolT, Salacone P., Gilio B., Gandini L., Dondero F., Jannini E., Lenzi A. An-drogens and fertility. J. Endocrinol. Invest. 2005; 28(3):51-55.
13. Mendis-Handagama S. Effects of thyroid hormones on Leydid cells in the postnatal testis //Histology and Histopathology. 2004. 19:985-997.
14. Odermatt A., Strajhar P., Engeli R.T. Disruption of steroidogenesis: cell models for mechanistic investigations and as screening tools. J. Steroid Biochem. Mol. Biol. 2016;158:9-21.
15. Ono Y., Ono S., Yasunaga H., Matsui H., Fushimi K., Tanaka Y. Clinical characteristics and outcomes of myxedema coma: analysis of a national inpatient database in Japan. J Epidemiol. (2017) 27:117-22.
16. Ozgtiner M., Senol A., Ural M., Ifler M. The histological changes caused by experimental hypothyroid in the testicular tissue of adult rats. Suleyman Demirel University. J Eacult. Med. 2004;11:1- 6.
17. Palace M.R. Perioperative management of thyroid dysfunction. Health Servl Insights. (2017) 10:1178632916689677.
18. Roger T., Cornelia F. Currently available murine leydigcell lines can be applied to study early steps of steroidogenesis bat not testosterone synthesis. Hellion, 2018.00527.
