Андрология и генитальная хирургия. 2023; 24: 150-156
Связь между уровнем цитокинов семенной плазмы и астенозооспермией у бесплодных мужчин
https://doi.org/10.17650/2070-9781-2023-24-1-150-156Аннотация
Введение. Нарушение мужской фертильности может иметь различные причины, одной из которых является снижение подвижности сперматозоидов. В патогенезе астенозооспермии могут быть задействованы некоторые биоактивные вещества, в частности цитокины.
Цель исследования – оценить количество наиболее изученных на сегодняшний день цитокинов в эякуляте бесплодных мужчин с нормозооспермией и пациентов с астенозооспермией.
Материалы и методы. Мы обследовали 38 бесплодных пациентов с нормозооспермией и 10 мужчин с астенозооспермией, сопоставимых по возрасту. Всем участникам исследования были выполнены спермограмма и анализ на определение количества цитокинов в семенной плазме: интерлейкинов 1β, 2, 4–8, 10, 12, 13, 17, G-CSF, GM-CSF, интерферона γ, MCP-1, MIP-1β и фактора некроза опухоли α. Затем методами непараметрической статистики проанализировали связь между уровнем вышеуказанных цитокинов и наличием астенозооспермии.
Результаты. Нам удалось выяснить, что существует связь между концентрацией интерлейкинов 6, 8 и MIP-1β и подвижностью сперматозоидов.
Заключение. Необходимы дальнейшие исследования, чтобы оценить, имеют ли данные биоактивные медиаторы воспаления потенциальную значимость для использования в качестве мишеней при лечении мужского бесплодия.
Список литературы
1. Aitken R.J., Best F.S.M., Richardson D.W. et al. An analysis of sperm function in cases of unexplained infertility: conventional criteria, movement characteristics, and fertilizing capacity. Fertil Steril 1982;38(2):212–21. DOI: 10.1016/S0015-0282(16)46462-9
2. Agarwal A., Saleh R.A. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002;29(4):817–27. DOI: 10.1016/s0094-0143(02)00081-2
3. Sharma R.K., Agarwal A. Role of reactive oxygen species in male infertility. Urology 1996;48(6):835–50. DOI: 10.1016/s0090-4295(96)00313-5
4. Sanocka D., Jedrzejczak P., Szumała-Kaekol A. et al. Male genital tract inflammation: the role of selected interleukins in regulation of pro-oxidant and antioxidant enzymatic substances in seminal plasma. J Androl 2003;24(3):448–55. DOI: 10.1002/j.1939-4640.2003.tb02693.x
5. Koçak I., Yenisey C., Dündar M. et al. Relationship between seminal plasma interleukin-6 and tumor necrosis factor alpha levels with semen parameters in fertile and infertile men. Urol Res 2002;30(4):263–7. DOI: 10.1007/s00240-002-0269-y
6. Gruschwitz M.S., Brezinschek R., Brezinschek H.P. Cytokine levels in the seminal plasma of infertile males. J Androl 1996;17(2):158–63.
7. Elfassy Y., Bongrani A., Levy P. et al. Relationships between metabolic status, seminal adipokines, and reproductive functions in men from infertile couples. Eur J Endocrinol 2020;182(1):67–7. DOI: 10.1530/EJE-19-0615
8. Grande G., Milardi D., Baroni S. et al. Identification of seminal markers of male accessory gland inflammation: from molecules to proteome. Am J Reprod Immunol 2018;80(2):e12992. DOI: 10.1111/aji.12992
9. Seshadri S., Bates M., Vince G., Jones D.I.L. The role of cytokine expression in different subgroups of subfertile men. Am J Reprod Immunol 2009;62(5):275–82. DOI: 10.1111/j.1600-0897.2009.00736.x
Andrology and Genital Surgery. 2023; 24: 150-156
Relationship between seminal plasma cytokine levels and asthenozoospermia in infertile men
https://doi.org/10.17650/2070-9781-2023-24-1-150-156Abstract
Background. Violation of male fertility can have various causes, one of which is a decrease in sperm motility. In the pathogenesis of asthenozoospermia, some bioactive substances, in particular cytokines, may be involved.
Aim. To estimate the number of the most studied cytokines to date in the ejaculate of infertile men with normozoospermia and patients with asthenozoospermia.
Materials and methods. We examined 38 infertile patients with normozoospermia and 10 men with asthenozoospermia, comparable in age. All participants in the study were performed a spermogram and an analysis to determine the amount of the cytokines in seminal plasma, such as interleukins 1β, 2, 4–8, 10, 12, 13, 17, G-CSF, GM-CSF, interferon γ, MCP-1, MIP-1β and tumor necrosis factor α. Then, using nonparametric statistics, the relationship between the level of the above cytokines and the presence of asthenozoospermia was analyzed.
Results. As a result, we were able to find out that there is a relationship between the concentration of interleukin 6, interleukin 8 and MIP-1β and sperm motility.
Conclusion. Further research is needed to assess whether these bioactive inflammatory mediators have potential relevance for use as targets in the treatment of male infertility.
References
1. Aitken R.J., Best F.S.M., Richardson D.W. et al. An analysis of sperm function in cases of unexplained infertility: conventional criteria, movement characteristics, and fertilizing capacity. Fertil Steril 1982;38(2):212–21. DOI: 10.1016/S0015-0282(16)46462-9
2. Agarwal A., Saleh R.A. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002;29(4):817–27. DOI: 10.1016/s0094-0143(02)00081-2
3. Sharma R.K., Agarwal A. Role of reactive oxygen species in male infertility. Urology 1996;48(6):835–50. DOI: 10.1016/s0090-4295(96)00313-5
4. Sanocka D., Jedrzejczak P., Szumała-Kaekol A. et al. Male genital tract inflammation: the role of selected interleukins in regulation of pro-oxidant and antioxidant enzymatic substances in seminal plasma. J Androl 2003;24(3):448–55. DOI: 10.1002/j.1939-4640.2003.tb02693.x
5. Koçak I., Yenisey C., Dündar M. et al. Relationship between seminal plasma interleukin-6 and tumor necrosis factor alpha levels with semen parameters in fertile and infertile men. Urol Res 2002;30(4):263–7. DOI: 10.1007/s00240-002-0269-y
6. Gruschwitz M.S., Brezinschek R., Brezinschek H.P. Cytokine levels in the seminal plasma of infertile males. J Androl 1996;17(2):158–63.
7. Elfassy Y., Bongrani A., Levy P. et al. Relationships between metabolic status, seminal adipokines, and reproductive functions in men from infertile couples. Eur J Endocrinol 2020;182(1):67–7. DOI: 10.1530/EJE-19-0615
8. Grande G., Milardi D., Baroni S. et al. Identification of seminal markers of male accessory gland inflammation: from molecules to proteome. Am J Reprod Immunol 2018;80(2):e12992. DOI: 10.1111/aji.12992
9. Seshadri S., Bates M., Vince G., Jones D.I.L. The role of cytokine expression in different subgroups of subfertile men. Am J Reprod Immunol 2009;62(5):275–82. DOI: 10.1111/j.1600-0897.2009.00736.x
