Андрология и генитальная хирургия. 2022; 23: 30-36
Хемилюминесцентный анализ свободнорадикальных процессов в семенной плазме мужчин с патоспермией, перенесших инфекцию COVID-19
https://doi.org/10.17650/1726-9784-2022-23-1-30-36Аннотация
Введение. В условиях пандемии COVID-19 актуальным представляется исследование клинических особенностей и влияния вируса SARS-CoV-2 на репродуктивную систему и фертильность мужчин.
Цель – оценить интенсивность окислительных процессов в семенной плазме мужчин с патозооспермией после перенесенной инфекции COVID-19.
Материалы и методы. Параметры спермограммы оценены в соответствии с критериями руководства Всемирной организации здравоохранения 2010 г. (5-е издание) по исследованию и обработке эякулята человека через 2–3 мес после получения пациентом отрицательного результата полимеразной цепной реакции назофарингеального мазка на ДНК вируса SARS-CoV-2. Методом хемилюминесценции, индуцированной ионами двухвалентного железа, исследована интенсивность свободнорадикальных процессов в семенной плазме.
Результаты. Установлено снижение подвижности сперматозоидов и повышение количества лейкоцитов у мужчин с патозооспермией, перенесших инфекцию COVID-19. Наблюдаются более низкие уровни таких показателей, как объем эякулята, общая концентрация сперматозоидов и количество морфологически нормальных форм сперматозоидов. Выявлена повышенная способность к генерации активных форм кислорода в семенной плазме при патозооспермии и COVID-19.
Выводы. Полученные результаты свидетельствуют о том, что SARS-CoV-2 влияет на мужскую половую систему прямым или косвенным образом и оказывает негативное воздействие на мужское репродуктивное здоровье, вызывая активацию процессов липопероксидации и сперматогенную недостаточность.
Список литературы
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19. Azadi L., Tavalaee M., Deemeh M.R. et al. Effects of Tempol and Quercetin on Human Sperm Function after Cryopreservation. Cryo Letters 2017;38(1):29–36.
20. Agarwal A., Ahmad G., Sharma R. Reference values of reactive oxygen species in seminal ejaculates using chemiluminescence assay. J Assist Reprod Genet 2015;32(12):1721–9. DOI: 10.1007/s10815-015-0584-1.
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Andrology and Genital Surgery. 2022; 23: 30-36
Chemiluminescence analysis of free radical processes in seminal plasma of men with pathospermia who have undergone COVID-19 infection
https://doi.org/10.17650/1726-9784-2022-23-1-30-36Abstract
Introduction. In the context of the COVID-19 pandemic, it seems relevant to study the clinical features and the effect of the SARS-CoV-2 virus on the reproductive system and male fertility.
The study objective was to assess the intensity of oxidative processes in the seminal plasma of men with pathozoospermia after suffering a COVID-19 infection.
Materials and methods. Spermogram parameters were assessed in accordance with the criteria of the 2010 WHO Guidelines, 5th edition, for the study and processing of human ejaculate two to three months after the patient received a negative polymerase chain reaction result of a nasopharyngeal swab for SARS-CoV-2 DNA. The intensity of free radical processes in seminal plasma was investigated by the method of chemiluminescence induced by ions of bivalent iron.
Results. A decrease in sperm motility and an increase in the number of leukocytes were found in men with pathozoospermia who had undergone COVID-19 infection. There are lower levels of indicators such as ejaculate volume, total sperm concentration and the number of morphologically normal sperm forms. An increased ability to generate reactive oxygen species in seminal plasma was revealed in pathozoospermia and COVID-19.
Conclusions. The results obtained indicate that SARS-CoV-2 affects the male reproductive system directly or indirectly and has a negative effect on male reproductive health, causing the activation of lipid peroxidation processes and spermatogenic insufficiency.
References
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19. Azadi L., Tavalaee M., Deemeh M.R. et al. Effects of Tempol and Quercetin on Human Sperm Function after Cryopreservation. Cryo Letters 2017;38(1):29–36.
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21. Kulchenko N., Pashina N. Association of leukocyte activity and DNA fragmentation in men with nonobstructive azoospermia. Georgian Med News 2020;(299):26–9.
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24. Li H., Xiao X., Zhang J. et al. Impaired spermatogenesis in COVID-19 patients. EClinicalMedicine 2020;28:100604. DOI: 10.1016/j.eclinm.2020.100604.
