Андрология и генитальная хирургия. 2017; 18: 27-32
ОКСИДАТИВНЫЙ СТРЕСС И ПАТОЗООСПЕРМИЯ
https://doi.org/10.17650/2070-9781-2017-18-2-27-32Аннотация
Цель работы – выявить уровень оксидативного стресса и антиоксидантной защиты эякулята при различных видах патозооспермии, обусловленной заболеваниями органов репродуктивной системы, включая варикоцеле, идиопатическую астенозооспермию, необструктивную азооспермию. Группы включали по 14, 11 и 16 мужчин в возрасте 20–45 лет.
Методы исследования эякулята: изучение морфологических параметров по рекомендациям Всемирной организации здравоохранения 5-го издания. Oпределение биохимических параметров спермоплазмы проводили по стандартным методам, описанным в ранее проведенных работах.
В исследование включали мужчин, в эякуляте которых были обнаружены нарушения подвижности и морфологии сперматозоидов, т. е. диагностировалась патозооспермия в форме астенотератозооспермии. Морфологические и биохимические изменения отмечены в группах больных с варикоцеле и с астенои азооспермией по сравнению с группой нормозооспермии.
В отдельно выделенной группе с варикоцеле обследование пациентов проводили до и после варикоцелэктомии. Морфологические параметры эякулята не претерпели существенных улучшений, однако по биохимическим параметрам спермоплазмы были выявлены более значительные изменения: повышение общей антиоксидантной активности, снижение уровня супероксиддисмутазы как свидетельство ослабления влияния оксидативного стресса после варикоцелэктомии.
Список литературы
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22. Altunoluk B., Efe E., Kurutas E.D. et al. Elevation of both reactive oxygen species and antioxidant enzimes in vein tissue of infertile men with varicocele. Urol Int 2012;88(1):102–6. DOI: 10.1159/000332156.
23. Hamada A., Esteves S.C., Agarval A. et al. Insight into oxidative stress in varicocele-associated male infertility: part 2. Nat Rev Urol 2013;10(1):26–37. DOI: 10.1038/nrurol.2012.198.
24. Valko M., Leibfritz D., Moncol J. et al. Free radicals and antioxidans in normal physiological functhions and human disease. Int J Biochem Cell Biol 2007;39:44–8. DOI: 10.1016/j.biocel.2006.07.001.
Andrology and Genital Surgery. 2017; 18: 27-32
OXIDATIVE STRESS AND SPERM PATHOLOGIES
https://doi.org/10.17650/2070-9781-2017-18-2-27-32Abstract
The study objective was to evaluate the level of oxidative stress and antioxidant defense of the ejaculate in different types of sperm pathologies caused by reproductive system disorders including varicocele, idiopathic asthenozoospermia, non-obstructive asthenozoospermia. Patients groups included 14, 11, and 16 men aged 20–45.
Methods of ejaculate examination included study of morphological parameters in accordance with the 5th edition of the World Health Organization Guidelines. Biochemical parameters of the spermoplasm were measured according to the standard procedures described in previous articles.
The study included men with abnormal sperm motility and morphology in the ejaculate, i. e. men with sperm pathologies in the form of asthenozoospermia. Morphological and biochemical changes were detected in the patient groups with varicocele and with asthenoand azoospermia compared to the normospermia group.
In the separate varicocele group, patients were examined before and after varicocelectomy. Morphological parameters of the ejaculate didn’t show significant improvement, but biochemical parameters of the spermoplasm changed significantly: total antioxidant activity increased, the level of superoxide dismutase decreased which demonstrates decreased effect of oxidative stress after varicocelectomy.
References
1. Agarval A., Said T.M. Role of sperm chromatin abnormalities and DNA damage in male fertility. Hum Reprod 2003;19(4):331–45. PMID: 12926527.
2. Tremellen K. Oxidative stress and male infertility – a clinical perspective. Hum Reprod Update 2008;14(3):243–58. DOI: 10.1093/humupd/dmn004.
3. Chen S.S., Huang W.J., Chang L.S. et al. Attenuation of oxidative stress after varicocelectomy in subfertile patients with varicocele. J Urol 2008;179(2):639–42. DOI: 10.1016/j.juro.2007.09.039.
4. Pankratova M.S., Yusipovich A.I., Vorontsova M.V. i dr. Osobennosti kislorodnogo i antioksidantnogo statusa krovi na fone zamestitel'noi terapii gormonom rosta u detei s somatotropnoi nedostatochnost'yu. Klinicheskaya endokrinologiya 2012;(5):10–5. [Pankratova M.S., Yusipovich A.I., Vorontsova M.V. et al. Features of oxygen and antioxidant status of blood on the background of substitution therapy with growth hormone in children with growth failure. Klinicheskaya endokrinologiya = Clinical Endocrinology 2012;(5):10–5. (In Russ.)].
5. Sharma R.K., Agarval A. Role of reactive oxygen species in male infertility. Urology 1996;48(6):835–50. PMID: 8973665.
6. Seli E., Gardner D.K., Sakkas D. et al. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril 2004;82(2):378–83. DOI: 10.1016/j.fertnstert.2003.12.039.
7. Piomboni P., Gambera L., Serafini F. et al. Sperm quality improvement after natural antioxidant tretment of asthenoterato-spermic men with leucocytospermia. Asian J Androl 2008;10(2):201–6. DOI: 10.1111/j.1745-7262.2008.00356.x.
8. Wang X., Sharma R.K., Sikka S.C. et al. Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility. Fertil Steril 2003;80(3):531–5. PMID: 12969693.
9. Meseguer M., Pellicer A., Garrido N. et al. The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility prognoctic factor. Fertil Steril 2007;8:415–20. PMID: 12935955.
10. Lachaud C., Tesarik J., Cacadas M.L. et al. Apoptosis and necrosis in human ejaculated spermatozoa. Hum Reprod 2004;19(3):607–10. DOI: 10.1093/humrep/deh130.
11. Sadek A., Almohamdy A.S., Zaki A. et al. Sperm chromatin condensation in infertile men with varicocele before and after surgical repair. Fertil Steril 2011;95(5):1705–8. DOI: 10.1016/j.fertnstert.2011.01.008.
12. Henkel R.R. Leukocytes and oxidative stress: dilemma for sperm function and male fertility. Asian J Androl 2011;13: 43–52. DOI: 10.1038/aja.2010.76.
13. Ershova O.A., Bairova T.A., Kolesnikov S.I. i dr. Okislitel'nyi stress i gen katalazy. Byulleten' eksperimental'noi biologii i meditsiny 2016;161(3):378–81. [Ershova O.A., Bairova T.A., Kolesnikov S.I. et al. Oxidative stress and catalase gene. Bulleten’ eksperimental’noy biologii i meditsiny = Bulletin of Experimental Biology and Medicine 2016;161(3):378–81. (In Russ.)].
14. Smith R., Kaune H., Parodi D. et al. Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Hum Reprod 2006;21(4): 986–93. DOI: 10.1093/humrep/dei429.
15. Rukovodstvo VOZ po issledovaniyu i obrabotke eyakulyata cheloveka. 5-e izd. M., 2012. [The WHO Guidelines for the study and treatment of human sperm. 5th edn. Moscow, 2012. (In Russ.)].
16. Henkel R., Kierspel E., Stalf T. et al. Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in nonleukocytospermic patients. Fertil Steril 2005;83(3):635–42. DOI: 10.1016/j.fertnstert.2004.11.022.
17. Gharagozloo P., Aitken R.J. The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Hum Reprod 2011;26(7):1628–40. DOI: 10.1093/humrep/der132.
18. Bozhedomov V.A., Toroptseva M.V., Ushakova I.V. i dr. Aktivnye formy kisloroda i reproduktivnaya funktsiya muzhchin: fundamental'nye i klinicheskie aspekty (obzor literatury). Andrologiya i genital'naya khirurgiya 2011;(3):10–6. [Bozhedomov V.A., Toroptseva M.V., Ushakova I.V. et al. Reactive oxygen species and reproductive function in men: basic and clinical aspects (review of literature). Andrologiya i genital’naya khirurgiya = Andrology and Genital Surgery 2011;(3):10–6. (In Russ.)].
19. Showell M.G., Brown J., Yazdani A. et al. Antioxidants for male subfertility. Cochrane Database Syst Rev 2011;19(1):CD007411. PMID: 21249690.
20. Baaseem A., Belzile E., Ciampi A. et al. Varicocele and male factor infertility treatment. Eur Urol 2011;60(4):796–808. DOI: 10.1016/j.eururo.2011.06.018.
21. Agarval A., Prabakaran S., Allamaneni S.S. et al. Relationship between oxidative stress, varicocele and infertility: a meta-analysis. Reprod Biomed Online 2006;12(5):630–3. PMID: 16790111.
22. Altunoluk B., Efe E., Kurutas E.D. et al. Elevation of both reactive oxygen species and antioxidant enzimes in vein tissue of infertile men with varicocele. Urol Int 2012;88(1):102–6. DOI: 10.1159/000332156.
23. Hamada A., Esteves S.C., Agarval A. et al. Insight into oxidative stress in varicocele-associated male infertility: part 2. Nat Rev Urol 2013;10(1):26–37. DOI: 10.1038/nrurol.2012.198.
24. Valko M., Leibfritz D., Moncol J. et al. Free radicals and antioxidans in normal physiological functhions and human disease. Int J Biochem Cell Biol 2007;39:44–8. DOI: 10.1016/j.biocel.2006.07.001.
