Андрология и генитальная хирургия. 2015; 16: 51-55
Опыт применения докозагексаеновой кислоты (БрудиПлюс) у пациентов с повышенным индексом фрагментации ДНК сперматозоидов в Научном центре акушерства, гинекологии и перинатологии им. акад. В.И. Кулакова
https://doi.org/10.17650/2070-9781-2015-16-2-51-55Аннотация
Почти в половине браков причиной бесплодия является мужской фактор. У бесплодных мужчин доля сперматозоидов с нарушением целостности ДНК более 30 %, при этом у здоровых фертильных мужчин – менее 15 %. С распространением вспомогательных репродуктивных технологий растет осознание важности повреждения ДНК спермы. На сегодняшний день эти последствия еще не изучены, а имеющийся терапевтический эффект от приема антиоксидантов не имеет прямой корреляции с уровнем фрагментации ДНК спермы. Докозагексаеновая кислота относится к наиболее ценным для здоровья человека полиненасыщенным жирным кислотам омега-3. Докозагексаеновая кислота – главный компонент серого вещества мозга, сетчатки глаза, яичек, спермы и клеточных мембран. В связи с этим было проведено исследование, целью которого явилась оценка влияния нутрицевтической энзиматической докозагексаеновой кислоты триглицерида (БрудиПлюс) в высокой концентрации на поврежденную ДНК сперматозоидов у пациентов с патозооспермией. В данное исследование были включены 40 больных с идиопатической патозооспермией и с уровнем фрагментации ДНК, который превышал нормативные значения. Были получены положительные результаты: прием БрудиПлюс позволяет снизить уровень повреждения ДНК сперматозоидов, а также улучшить антиоксидантную систему спермы.
Список литературы
1. Guzick D.S., Overstreet J.W., FactorLitvak P. et al. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2004;345(19):1388–93.
2. Osman A., Alsomait H., Seshadri S. et al. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysis. Reprod Biomed Online 2015;30(2):120–7.
3. Gandini L., Lombardo F., Paoli D. et al. Study of apoptotic DNA fragmentation in human spermatozoa. Hum Reprod 2000;15(4):830–9.
4. Evenson D.P. Sperm chromatin structure assay (SCSA®). Methods Mol Biol 2013; 927:147–64.
5. Kumar K., Deka D., Singh A. et al. Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception. J Assist Reprod Genet 2012;29(9):861–7.
6. Spano M., Bonde J.P., Hjøllund H.I. The treatment of obstructive azoospermia by intracytoplasmic sperm injection. Fertil Steril 2000;73(1):43–50.
7. Zini A., Libman J. Sperm DNA damage: importance in the era of assisted reproduction. Curr Open Urol 2006;16(6):428–34.
8. Zini A., Albert O., Robaire B. Assessing sperm chromatin and DNA damage: clinical importance and development of standards. Andrology 2014;2(3):322–5.
9. Nogales-Gadea G., Pinós T., Ruiz J.R. Are mitochondrial haplogroups associated with elite athletic status? A study on a Spanish cohort. Mitochondrion 2011;11(6):905–8.
10. Ahluwalia B., Holman R.T. Fatty acid composition of lipids of bull, boar, rabbit and human semen. J Reprod Fertil 1969;18(3):431–7.
11. Poulos A., White I.G. The phospholipid composition of human spermatozoa and seminal plasma. J Reprod Fertil 1973;35(2):265–72.
12. Lenzi A., Picardo M., Gandini L., Dondero F. Lipids of the sperm plasma membrane: from polyunsaturated fatty acids considered as markers of sperm function to possible scavenger therapy. Hum Reprod Update 1996;2(3):246–56.
13. Alvarez J.G., Storey B.T. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 1995;42(3):334–46.
14. Wang A.W., Zhang H., Ikemoto I. et al. Reactive oxygen species generation by seminal cells during cryopreservation. Urology 1997;49(6):921–5.
15. Alvarez J.G., Storey B.T. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. J Androl 1992;13(3): 232–41.
16. Lasso J.L., Noiles E.E., Alvarez J.G., Storey B.T. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. J Androl 1994;15(3): 255–65.
17. Gadea J., Molla M., Selles E. et al. Reduced glutathione content in human sperm is decreased after cryopreservation: effect of the addition of reduced glutathione to the freezing and thawing extenders. Cryobiology 2011;62(1):40–6.
18. Agarwal A., Sekhon L.H. The role of antioxidant therapy in the treatment of male infertility. Hum Fertil (Camb) 2010;13(4):217–25.
19. Safarinejad M.R. Effect of omega-3 polyunsaturated fatty acid supplementation on semen profile and enzymatic anti-oxidant capacity of seminal plasma in infertile men with idiopathic oligoasthenoteratospermia: a double-blind, placebo-controlled, randomised study. Andrologia 2011;43(1):38–47.
20. Zini A., Al-Hathal N. Antioxidant therapy in male infertility: fact or fiction? Asian J Androl 2011;13(3):374–81.
21. Larson-Cook K.L., Brannian J.D., Hansen K.A. et al. Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay. Fertil Steril 2003;80(4):895–902.
Andrology and Genital Surgery. 2015; 16: 51-55
Experience in the use of docosahexaenoic acid (BrudiPlus) in patients with increased sperm DNA fragmentation index in Acad. V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology
https://doi.org/10.17650/2070-9781-2015-16-2-51-55Abstract
Male factor is the reason of infertility in almost half of marriages. Infertile men have the percentage of sperm with violations of DNA integrity of over 30 %; with that, healthy fertile men have that indicator of less than 15 %. Understanding of importance of damages of sperm DNA is growing with distribution ofauxiliary reproductive technologies. As of today, these consequences have not been studies yet, and the therapeutic effect of intake of antioxidants has not direct correlation with the sperm DNA fragmentation level. Docosahexaenoic acid is one of the most valuable omega-3 polyunsaturated fatty acids for human health. Docosahexaenoic acid is the main component of the brain gray matter, retina, testes, and sperm cell membranes. In connection with that, a study was held the purpose of which was to assess the effect of the nutraceutical enzymatic docosahexaenoic acid triglyceride (BrudiPlus) in high concentrations on damaged sperm DNA of patients with idiopathic pathozoospermia. 40 patients with idiopathic pathozoospermia and the level of DNA fragmentation over the statutory value took part in this study. The following positive results were received: intake of BrudiPlus allowed decreasing sperm DNA damages and improving of antioxidant system of sperm.
References
1. Guzick D.S., Overstreet J.W., FactorLitvak P. et al. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2004;345(19):1388–93.
2. Osman A., Alsomait H., Seshadri S. et al. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysis. Reprod Biomed Online 2015;30(2):120–7.
3. Gandini L., Lombardo F., Paoli D. et al. Study of apoptotic DNA fragmentation in human spermatozoa. Hum Reprod 2000;15(4):830–9.
4. Evenson D.P. Sperm chromatin structure assay (SCSA®). Methods Mol Biol 2013; 927:147–64.
5. Kumar K., Deka D., Singh A. et al. Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception. J Assist Reprod Genet 2012;29(9):861–7.
6. Spano M., Bonde J.P., Hjøllund H.I. The treatment of obstructive azoospermia by intracytoplasmic sperm injection. Fertil Steril 2000;73(1):43–50.
7. Zini A., Libman J. Sperm DNA damage: importance in the era of assisted reproduction. Curr Open Urol 2006;16(6):428–34.
8. Zini A., Albert O., Robaire B. Assessing sperm chromatin and DNA damage: clinical importance and development of standards. Andrology 2014;2(3):322–5.
9. Nogales-Gadea G., Pinós T., Ruiz J.R. Are mitochondrial haplogroups associated with elite athletic status? A study on a Spanish cohort. Mitochondrion 2011;11(6):905–8.
10. Ahluwalia B., Holman R.T. Fatty acid composition of lipids of bull, boar, rabbit and human semen. J Reprod Fertil 1969;18(3):431–7.
11. Poulos A., White I.G. The phospholipid composition of human spermatozoa and seminal plasma. J Reprod Fertil 1973;35(2):265–72.
12. Lenzi A., Picardo M., Gandini L., Dondero F. Lipids of the sperm plasma membrane: from polyunsaturated fatty acids considered as markers of sperm function to possible scavenger therapy. Hum Reprod Update 1996;2(3):246–56.
13. Alvarez J.G., Storey B.T. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 1995;42(3):334–46.
14. Wang A.W., Zhang H., Ikemoto I. et al. Reactive oxygen species generation by seminal cells during cryopreservation. Urology 1997;49(6):921–5.
15. Alvarez J.G., Storey B.T. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. J Androl 1992;13(3): 232–41.
16. Lasso J.L., Noiles E.E., Alvarez J.G., Storey B.T. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. J Androl 1994;15(3): 255–65.
17. Gadea J., Molla M., Selles E. et al. Reduced glutathione content in human sperm is decreased after cryopreservation: effect of the addition of reduced glutathione to the freezing and thawing extenders. Cryobiology 2011;62(1):40–6.
18. Agarwal A., Sekhon L.H. The role of antioxidant therapy in the treatment of male infertility. Hum Fertil (Camb) 2010;13(4):217–25.
19. Safarinejad M.R. Effect of omega-3 polyunsaturated fatty acid supplementation on semen profile and enzymatic anti-oxidant capacity of seminal plasma in infertile men with idiopathic oligoasthenoteratospermia: a double-blind, placebo-controlled, randomised study. Andrologia 2011;43(1):38–47.
20. Zini A., Al-Hathal N. Antioxidant therapy in male infertility: fact or fiction? Asian J Androl 2011;13(3):374–81.
21. Larson-Cook K.L., Brannian J.D., Hansen K.A. et al. Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay. Fertil Steril 2003;80(4):895–902.
